Transformation of rice mediated by Agrobacterium tumefaciens

Hiei, Yukoh; Komari, Toshihiko; Kubo, Tomoaki

doi:10.1007/978-94-011-5794-0_20

Cited by 275 publications

(202 citation statements)

References 114 publications

Supporting

Mentioning

187

Contrasting

Unclassified

Order By: Relevance

“…Successful Agrobacterium-mediated transformation has been reported in rice (Aldemita and Hodges, 1996;Hiei et al, 1997;Hiei et al, 1994;Rashid et al, 1996;Toki et al, 1997), maize (Ishida et al, 1996), barley (Tingay et al, 1997), wheat Cheng et al, 1997) and, recently, sorghum (Zhao et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…The multiplicity of factors that influence transformation is probably the reason why Agrobacterium-mediated transformation in monocotyledonous plant species has been difficult to achieve (Hiei et al, 1997;Ishida et al, 1996). Several factors are important in transformation, the type and developmental stage of the infected plant tissues, the concentration of Agrobacterium tumefaciens, the composition of the media used for co-cultivation infection and tissue culture, the selectable marker genes used, the type of vector and the plant genotype being among the factors that influence transformation (reviewed by Hiei et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Several factors are important in transformation, the type and developmental stage of the infected plant tissues, the concentration of Agrobacterium tumefaciens, the composition of the media used for co-cultivation infection and tissue culture, the selectable marker genes used, the type of vector and the plant genotype being among the factors that influence transformation (reviewed by Hiei et al, 1997). A critical point in developing an efficient transformation protocol is to find the right combination of the many factors that act together during transformation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

et al. 2004

View full text Add to dashboard Cite

The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Agrobacterium-mediated transformation is a general method for genetic modification in many plant species because it allows efficient insertion of stable, unrearranged, single-copy sequences into plant genomes (Nishimura et al 2006). Efficient methods for Agrobacterium-mediated transformation of japonica rice have been developed (Hiei et al 1994, Hiei et al 1997, Hiei and Komari 2008. Two japonica varieties, Nipponbare and Kitaake (developed in Japan), are good genotypes for Agrobacterium-mediated transformation.…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that transformation efficiencies are genotype dependent (Hiei et al 1997, Hiei andKomari 2008). Although efficient procedures for Agrobacteriummediated transformation of either japonica or indica rice have been developed, the transgenic efficiency of the rice varieties developed in northern China have been completely unknown Komari 2008, Saika andToki 2010).…”

Section: Introductionmentioning

confidence: 99%

Differential transformation efficiency of Japonicarice varieties developed in northern China

Ли

Sun

et al. 2015

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective

Yang

Liu

Jiang

et al. 2016

Biotechnology Journal

View full text Add to dashboard Cite

The species of Chlorella represent a highly specialized group of green microalgae that can produce high levels of protein. Many Chlorella strains can grow rapidly and achieve high cell density under controlled conditions and are thus considered to be promising protein sources. Many advances in the genetic engineering of Chlorella have occurred in recent years, with significant developments in successful expression of heterologous proteins for various applications. Nevertheless, a lot of obstacles remain to be addressed, and a sophisticated and stable Chlorella expression system has yet to emerge. This review provides a brief summary of current knowledge on Chlorella and an overview of recent progress in the genetic engineering of Chlorella, and highlights the advances in the development of a genetic toolbox of Chlorella for heterologous protein expression. Research directions to further exploit the Chlorella expression system with respect to both challenges and perspectives are also discussed. This paper serves as a comprehensive literature review for the Chlorella community and will provide valuable insights into future exploration of Chlorella as a promising host for heterologous protein expression.

show abstract

Transformation of rice mediated by Agrobacterium tumefaciens

Cited by 275 publications

References 114 publications

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

Differential transformation efficiency of Japonicarice varieties developed in northern China

Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective

Contact Info

Product

Resources

About