Transformation of Primary Chick Embryo Fibroblasts by Marek's Disease Virus

Buranathai, Chantanee; Rodríguez, José; Grose, Charles

doi:10.1006/viro.1997.8854

Cited by 18 publications

(11 citation statements)

References 35 publications

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“…MDV oncogene(s), if existing, are thus most likely derived from the divergent repeat region and from genes unique to serotype I MDV. Previously, MDV has been shown to be capable of transforming CEF, when lytic infection is suppressed (21). Meq is predominantly expressed in latent or tumor cells (22), and it was demonstrated that blocking the expression of Meq reduces the anchorage-independent growth of an MDV-transformed cell line (23).…”

Section: Discussionmentioning

confidence: 99%

Marek's disease virus Meq transforms chicken cells via the v-Jun transcriptional cascade: A converging transforming pathway for avian oncoviruses

Levy

Gilad

Xia

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

Marek's disease virus Meq transforms chicken cells via the v-Jun transcriptional cascade: A converging transforming pathway for avian oncoviruses

Levy

Gilad

Xia

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…These lymphoblastoid cell lines are often considered the equivalent of latently infected cells, based on the limited expression of viral genes. In addition to the lymphoblastoid cell lines, an MDV-transformed chicken embryo fibroblast (CEF) cell line has been established, but this cell line also expresses late MDV genes, such as that for glycoprotein B (gB) (5).…”

Section: Marek's Disease (Md) Is a Highly Contagious Lymphoproliferatmentioning

confidence: 99%

Transactivation of Latent Marek's Disease Herpesvirus Genes in QT35, a Quail Fibroblast Cell Line, by Herpesvirus of Turkeys

Yamaguchi

Kaplan

Wakenell

et al. 2000

J Virol

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(62) showed that MDV and HVT can transactivate the promoter region in the long terminal repeat (LTR) of Rous sarcoma virus. Furthermore, they reported that HVT can efficiently transactivate the ICP4 and beta-thymidine kinase gene promoters of HSV as well as the promoter of the IE gene of human cytomegalovirus.More than 200 lymphoblastoid cell lines have been established from MD tumors (1, 7-9, 32, 35, 39). Most of these cell lines are CD4 ϩ CD8 Ϫ T cells (56). These lymphoblastoid cell lines are often considered the equivalent of latently infected cells, based on the limited expression of viral genes. In addition to the lymphoblastoid cell lines, an MDV-transformed chicken embryo fibroblast (CEF) cell line has been established, but this cell line also expresses late MDV genes, such as that for glycoprotein B (gB) (5).Three groups of transcripts located in the repeat regions of MDV in MD lymphoblastoid cell lines have been described. The first group consists of the latency-associated transcripts (LATs), which are antisense to ICP4 (see Fig. 1). Several groups have reported the presence of LATs in MD lymphomas and MD-derived lymphoblastoid cell lines (10,11,21,22,27,33); some of these (the MD small RNAs [MSRs]) are poly(A) negative and are retained in the nuclei (11), similar to the 1.5-and 2.0-kb LATs described for HSV (64). Interestingly, the MSRs are also found in nuclei of CEF productively infected with MDV.The second and third group of transcripts described for MD lymphomas and lymphoblastoid cell lines are located in the I 2 ,

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“…Only serotypes 1 strains of MDV are oncogenic though the transformation mechanism by MDV1 has not been fully understood [1,22]. An MDV1-specific gene, meq, has been identified, and its gene product, Meq, a basic leucine zipper (bZIP) oncoprotein, has been detected in all tumor samples and established MDV-transformed Tcell lines examined thus far, suggesting that Meq protein plays an important role in the transformation by MDV [19,20,22,23].…”

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confidence: 99%

The Detection of the meq Gene in Chicken Infected with Marek's Disease Virus Serotype 1.

Chang

Lee

Ohashi

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. In the genome of strains of very virulent Marek's disease virus serotype 1(vvMDV1), such as Md5 and RB1B, the meq open reading frame (ORF) encoding a 339-amino-acid bZIP protein, is present, while a slightly longer meq ORF, termed as L-meq, in which a 180-bp sequence is inserted into the meq ORF is found in other strains of MDV1, such as CVI988/R6 and attenuated JM. When chickens were infected with vvMDV1 strains and the meq gene was amplified by nested polymerase chain reaction (PCR), the meq gene was detected throughout the experimental period for 7 weeks post inoculation (pi). However, the L-meq gene was also detected at 3 to 5 weeks and 3 to 4 weeks pi. in Md5-infected and RB1B-infected chickens, respectively. In the case of chickens infected with an attenuated MDV1, the JM strain, the L-meq gene was detected at 2 to 7 weeks pi., and the meq gene was also detected at 2 to 6 weeks pi. Both L-meq and meq genes were detected in chickens infected with an attenuated nononcogenic vaccine strain of MDV1 (CVI988/R6), throughout the experimental period. Though quantitative PCR was not performed, a larger amount of the PCR products corresponding to the L-meq than the meq gene was amplified from chickens infected with JM or CVI988/R6. These results suggest that a dynamic population shift between the MDV subpopulations displaying meq and L-meq genes occurs in chickens during the course of MDV infection. Since the MDV subpopulation that displays the L-meq gene only displays it during the latent phase, the L-meq and its gene product, if any, might contribute to the maintenance of the MDV latency.

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Transformation of Primary Chick Embryo Fibroblasts by Marek's Disease Virus

Cited by 18 publications

References 35 publications

Marek's disease virus Meq transforms chicken cells via the v-Jun transcriptional cascade: A converging transforming pathway for avian oncoviruses

Marek's disease virus Meq transforms chicken cells via the v-Jun transcriptional cascade: A converging transforming pathway for avian oncoviruses

Transactivation of Latent Marek's Disease Herpesvirus Genes in QT35, a Quail Fibroblast Cell Line, by Herpesvirus of Turkeys

The Detection of the meq Gene in Chicken Infected with Marek's Disease Virus Serotype 1.

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