Transformation of Potato (Solanum tuberosumcv.Savalan) by Chitinase and Î²-1,3-Glucanase Genes of Myco-Parasitic Fungi Towards Improving Resistance to Rhizoctonia solani AG-3

Esfahani, Kasra; Motallebi, Mostafa; Zamani, Mohammad; Sohi, Haleh Hashemi; Jourabchi, Esmat

doi:10.29252/ijb.8.2.7104

Cited by 11 publications

(1 citation statement)

References 47 publications

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“…Several reports have demonstrated the use of pathogenesis related proteins to overcome pathogenic fungi in crop plants. Esfahani et al (2010) transformed potato (S. tuberosum cv. Savalan) with chitinase and β-1,3-glucanase genes of mycoparasitic fungi origin to create resistance against Rhizoctonia solani AG-3.…”

Section: Discussionmentioning

confidence: 99%

Journal of Plant Protection Research

Fatima,

Tabassum,

Yousaf

et al. 2019

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Potato (Solanum tuberosum L.), an important food crop in the world, is susceptible to many fungal pathogens including Alternaria solani and Fusarium oxysporum causing Fusarium wilt and early blight diseases. Mycoparasitic fungi like Trichoderma encode chitinases, cell wall degrading enzymes, with high antifungal activity against a wide range of phytopathogenic fungi. In this study, a binary vector harboring endochitinase gene of ~1,000 bp was constructed and used to transform potato nodes through Agrobacterium-mediated transformation. Out of several primary transformants, two transgenic potato lines were verified for transgene insertion and integration by Southern blot. In a pot experiment for Fusarium resistance, the transgenic potato lines didn't show any symptoms of disease, instead they remained healthy post infection. The transgenic potato lines exhibited 1.5 fold higher mRNA expression of endochitinase at 7 days as compared to 0 day post fungus inoculation. It was evident that the mRNA expression decreased over days of inoculation but was still higher than at 0 day and remained stable upto 30 days post inoculation. Similarly, for A. solani infection assay, the mRNA expression of the endochitinase gene was 3 fold higher 7 days post inoculation compared to expression at 0 day. Although the expression decreased by1.2 fold during subsequent days post infection, it remained stable for 30 days, suggesting that protection in transgenic potato plants against fungal pathogens was achieved through an increase in endochitinase transcript.

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Section: Discussionmentioning

confidence: 99%

Journal of Plant Protection Research

Fatima,

Tabassum,

Yousaf

et al. 2019

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Control of Fungal Diseases in Agricultural Crops by Chitinase and Glucanase Transgenes

Sandhu

Sidhu

Yadav

2017

Sustainable Agriculture Reviews

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Differential gene induction in resistant and susceptible potato cultivars at early stages of infection by Phytophthora infestans

Orłowska

Fiil

Kirk³

et al. 2011

Plant Cell Rep

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Sarpo Mira, a potato variety with high resistance against the late blight pathogen Phytophthora infestans, is being used in breeding programs to increase late blight resistance in commercial varieties. Discovering genes that are important for P. infestans resistance will assist in the development of molecular markers for the selection of new resistant cultivars and the use of resistant varieties will reduce the environmental, health and financial costs associated with the use of pesticides. Using complementary DNA amplified fragment length polymorphism analyses, differentially expressed genes involved in the potato-P. infestans interaction were identified in the susceptible Bintje and in the resistant Sarpo Mira potato cultivars. Forty-eight differentially expressed transcript derived fragments (TDFs) were cloned and sequenced. The expression profiles of some of these genes were analyzed in detail using quantitative RT-PCR at seven time points: 1, 4, 17, 24, 30, 41 and 65 hours after inoculation (hai). We found that five transcripts with homologies to pathogenesis/defense-related genes and two TDFs with homology to transcription factors were significantly induced to higher levels in the resistant cultivar at very early stages of the infection (1 hai). Interestingly, most of these genes showed different expression profiles throughout the whole infection process between both cultivars. Particularly during its biotrophic growth phase, P. infestans triggered the down-regulation of infection responsive genes in the susceptible but not in the resistance cultivar. Our results suggest that these newly identified early-induced transcripts may be good candidates for conferring Sarpo Mira's resistance to late blight and they could be useful molecular markers for the selection of new resistant cultivars.

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Transformation of Potato (Solanum tuberosumcv.Savalan) by Chitinase and Î²-1,3-Glucanase Genes of Myco-Parasitic Fungi Towards Improving Resistance to Rhizoctonia solani AG-3

Cited by 11 publications

References 47 publications

Journal of Plant Protection Research

Journal of Plant Protection Research

Control of Fungal Diseases in Agricultural Crops by Chitinase and Glucanase Transgenes

Differential gene induction in resistant and susceptible potato cultivars at early stages of infection by Phytophthora infestans

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