Transformation of Organic Matter by a Microbial Community in Sediments of Lake Baikal under Experimental Thermobaric Conditions of Protocatagenesis

Pavlova, Olga; Zemskaya, Т. I.; Lomakina, A. V.; Shubenkova, O. V.; Manakov, Andrey Yu.; Москвин, В. И.; Морозов, И. В.; Bukin, S. V.; Khlystov, Oleg

doi:10.1080/01490451.2015.1069910

Cited by 6 publications

(12 citation statements)

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“…Content and composition of TOC were detected in the samples of bottom sediments before (considering the organic substrates added) and at the end of the experiment. Preparation of the samples, identification of the content of organic carbon (C org , %), isolation of soluble (bitumoid) organic matter and GC–MS analyses were performed as described previously ( Pavlova et al, 2016 ). All measurements were triplicated.…”

Section: Methodsmentioning

confidence: 99%

The Ability of Microbial Community of Lake Baikal Bottom Sediments Associated with Gas Discharge to Carry Out the Transformation of Organic Matter under Thermobaric Conditions

et al. 2016

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The ability to compare the composition and metabolic potential of microbial communities inhabiting the subsurface sediment in geographically distinct locations is one of the keys to understanding the evolution and function of the subsurface biosphere. Prospective areas for study of the subsurface biosphere are the sites of hydrocarbon discharges on the bottom of the Lake Baikal rift, where ascending fluxes of gas-saturated fluids and oil from deep layers of bottom sediments seep into near-surface sediment. The samples of surface sediments collected in the area of the Posolskaya Bank methane seep were cultured for 17 months under thermobaric conditions (80°C, 5 MPa) with the addition of complementary organic substrate, and a different composition for the gas phase. After incubation, the presence of intact cells of microorganisms, organic matter transformation and the formation of oil biomarkers was confirmed in the samples, with the addition of Baikal diatom alga Synedra acus detritus, and gas mixture CH4:H2:CO2. Taxonomic assignment of the 16S rRNA sequence data indicates that the predominant sequences in the enrichment were Sphingomonas (55.3%), Solirubrobacter (27.5%) and Arthrobacter (16.6%). At the same time, in heat-killed sediment and in sediment without any additional substrates, which were cultivated in a CH4 atmosphere, no geochemical changes were detected, nor the presence of intact cells and 16S rRNA sequences of Bacteria and Archaea. This data may suggest that the decomposition of organic matter under culturing conditions could be performed by microorganisms from low-temperature sediment layers. One possible explanation of this phenomenon is migration of the representatives of the deep thermophilic community through fault zones in the near surface sediment layers, together with gas-bearing fluids.

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Section: Methodsmentioning

confidence: 99%

The Ability of Microbial Community of Lake Baikal Bottom Sediments Associated with Gas Discharge to Carry Out the Transformation of Organic Matter under Thermobaric Conditions

et al. 2016

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“…In the process of microbial communities from Lake Baikal fault zones cultivation, we have isolated several pure cultures of thermophilic bacteria with unusual for their species metabolism. Unlike cold marine sediments where endospores of thermophiles belong to obligate anaerobes, mantaining their metabolism via fermentation of organic substrates or sulphate reduction (Hubert et al, 2010;Müller et al, 2014;Chakraborty et al, 2018), in Baikal sediments associated with seepages of gas-saturated fluids we found facultatively anaerobic thermophilic prokaryotes of the genera Paracoccus, Geobacillus and Thermaerobacter (Pavlova et al, 2016;Khanaeva et al, 2017;Pavlova et al, 2019b). Genomic studies confirmed the potential of Thermaerobacter PB12/4term pure culture for mixotrophic type of nutrition, which is uncharacteristic for the members of genus Thermaerobacter as obligate aerobes (Baturina et al, 2018).…”

Section: Resultsmentioning

confidence: 62%

“…), which could be transferred to the surface sediments with gas-saturated material from depths of several kilometres (Pavlova et al, 2019a). In cultivated samples of bottom sediments from methane seeps characterized by presence of a fluid convection loop we detected typical mesophilic inhabitants of bottom sediments with a "flexible" metabolism (Bukin et al, 2016;Pavlova et al, 2016). This composition probably allows adapting to the changes in environmental conditions during the burial of individual bottom sediment layers and the circulation of fluid flows.…”

Section: Resultsmentioning

confidence: 79%

“…The experiments have shown that long-term cultivation of microbial communities from bottom sediments of methane seeps Goloustnoye and Posolsk Bank, which were enriched with detritus of the Baikal diatom Synedra acus and CH 4 :H 2 :CO 2 gas mixture, leads to the destruction of algal biomass and formation of petroleum biomarkers, such as retene or gammacerene (Bukin et al, 2016;Pavlova et al, 2016). These data indicate the involvement of microorganisms in the formation of retene during the destruction of diatoms under milder than pyrolysis conditions.…”

Section: Resultsmentioning

confidence: 90%

“…We have shown the ability of some taxa from Lake Baikal deep bottom sediment layers to survive under conditions typical for protocatagenesis, as well as in typical involvement known for these taxa in organic matter transformation locations. The metagenomic analysis of DNA samples before and after the experiments allowed us to characterize the dominant and minor components of microbial communities and identify the putative main participants in the observed processes inhabiting deep bottom sediments of Lake Baikal (Bukin et al, 2016;Pavlova et al, 2016;Pavlova et al, 2019a). We have noticed that different geochemical conditions of bottom sediments associated with hydrocarbon discharge determine different compositions of microbial communities and, hence, the transformation degree of organic matter as well as spectrum of compounds resulting from the destruction of organic matter under thermobaric conditions.…”

Section: Resultsmentioning

confidence: 99%

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Experimental equipment for Lake Baikal deep biosphere microorganism’s exploration and some results obtained using this equipment

Pavlova

Manakov

Bukin

et al. 2019

LFWB

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The development of special autoclaves with automatic temperature control (Nikolaev Institute of Inorganic Chemistry) had enabled reproduction of Lake Baikal tectonically active zone typical conditions (80°C, 5 MPa). This article describes in details the equipment developed and demonstrates some results obtained using the equipment. In series of thermobaric experiments on the cultivation of bottom sediment microbial communities, we have determined the microbial community potential to transform organic matter via the formation of dibenzothiophenes, tri-and monoaromatic steroids as well as petroleum biomarkers (retene and gammacerene). The particular bottom sediment geochemical environment associated with hydrocarbon discharge imply the composition of microbial communities and, hence, the organic matter transformation degree as well as a compound spectrum resulting from the destruction of organic matter under thermobaric conditions. The presence of microorganisms with an unusual metabolism suggests the promising potential for such studies both in application to Lake Baikal and worldwide

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Research Progress and Challenges on Persistent Organic Pollutants in Lakes

Guo,

Ji,

et al. 2024

J. Earth Sci.

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Lakes are the main reservoirs of persistent organic pollutants (POPs) from land, atmosphere and rivers. POPs in lakes undergo complex exchange, transformation, and degradation between water-air-sediment-biota interfaces, which are constrained and regulated by various physical, chemical and biological factors. POPs can affect ecological conditions, chemical properties of water and sediments, and biodiversity of the lake system. Therefore, it is important to study the sources, migration, transformation, environmental behavior and ecological impacts of POPs in lake ecosystems. This review summarizes research progress on detection technologies, diversity and origins, historical records, migration and transformation, distribution patterns, degradation and toxic effects of POPs in lakes. Finally, future directions related to POPs in lakes were summarized.

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Transformation of Organic Matter by a Microbial Community in Sediments of Lake Baikal under Experimental Thermobaric Conditions of Protocatagenesis

Cited by 6 publications

References 40 publications

The Ability of Microbial Community of Lake Baikal Bottom Sediments Associated with Gas Discharge to Carry Out the Transformation of Organic Matter under Thermobaric Conditions

The Ability of Microbial Community of Lake Baikal Bottom Sediments Associated with Gas Discharge to Carry Out the Transformation of Organic Matter under Thermobaric Conditions

Experimental equipment for Lake Baikal deep biosphere microorganism’s exploration and some results obtained using this equipment

Research Progress and Challenges on Persistent Organic Pollutants in Lakes

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