Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits

Torre, Mariana Cecilia Pérez de la; Fernández, Paula; Greppi, Julián Alejandro; Coviella, María Andrea; Fernandez, Martín Nicolás; Astigueta, Francisco Horacio; Mata, Diego Alejandro; Trupkin, Santiago Ariel

doi:10.1016/j.scienta.2018.02.047

Cited by 12 publications

(6 citation statements)

References 82 publications

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“…Many efforts were achieved to genetically improve ornamental traits in several cultivars, with a main focused interest on carnation, rose, chrysanthemum and petunia. Such genetic modifications lead to distinct developmental and structural changes, like flower color, compact branching of internodes, flower longevity improvement, adventitious root development, changing flowering time and resistance to different biotic or abiotic stresses [197,198,199,200]. However, delaying of leaf senescence, which is an essential criterion of plant quality according to many ornamental breeders, has not been considered along these species.…”

Section: Main Tfs Associated With Leaf Senescence In Cropsmentioning

confidence: 99%

Transcription Factors Associated with Leaf Senescence in Crops

Luoni

Astigueta

Nicosia

et al. 2019

Plants

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Leaf senescence is a complex mechanism controlled by multiple genetic and environmental variables. Different crops present a delay in leaf senescence with an important impact on grain yield trough the maintenance of the photosynthetic leaf area during the reproductive stage. Additionally, because of the temporal gap between the onset and phenotypic detection of the senescence process, candidate genes are key tools to enable the early detection of this process. In this sense and given the importance of some transcription factors as hub genes in senescence pathways, we present a comprehensive review on senescence-associated transcription factors, in model plant species and in agronomic relevant crops. This review will contribute to the knowledge of leaf senescence process in crops, thus providing a valuable tool to assist molecular crop breeding.

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Section: Main Tfs Associated With Leaf Senescence In Cropsmentioning

confidence: 99%

Transcription Factors Associated with Leaf Senescence in Crops

Luoni

Astigueta

Nicosia

et al. 2019

Plants

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“…The ability of callus cultures to form roots was also seen by Feeney and Punja (2003) . Unfortunately, regeneration of plants from hairy roots, although possible in some species ( Crane et al., 2006 ; Lütken et al., 2012 ; de la Torre et al., 2018 ), is often quite challenging and has not yet been reported in Cannabis.…”

Section: The Prerequisites For Genome Editingmentioning

confidence: 99%

Challenges and potentials of new breeding techniques in Cannabis sativa

Ingvardsen

Brinch-Pedersen

2023

Front. Plant Sci.

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Cannabis sativa L. is an ancient crop used for fiber and seed production and not least for its content of cannabinoids used for medicine and as an intoxicant drug. Due to the psychedelic effect of one of the compounds, tetrahydrocannabinol (THC), many countries had regulations or bands on Cannabis growing, also as fiber or seed crop. Recently, as many of these regulations are getting less tight, the interest for the many uses of this crop is increasing. Cannabis is dioecious and highly heterogenic, making traditional breeding costly and time consuming. Further, it might be difficult to introduce new traits without changing the cannabinoid profile. Genome editing using new breeding techniques might solve these problems. The successful use of genome editing requires sequence information on suitable target genes, a genome editing tool to be introduced into plant tissue and the ability to regenerate plants from transformed cells. This review summarizes the current status of Cannabis breeding, uncovers potentials and challenges of Cannabis in an era of new breeding techniques and finally suggests future focus areas that may help to improve our overall understanding of Cannabis and realize the potentials of the plant.

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“…Rhizobium rhizogenes-mediated plant transformation has for the last 4 decades been intensively applied in many studies with the pursuit of introducing compact habit and reduced plant growth traits in plants, primarily ornamentals (Christensen et al, 2008;Pérez de la Torre et al, 2018). The rationale has often been to develop genetically stable compact plants as a bio-sustainable alternative to chemical growth retardants.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, several efficient genetic engineering approaches exist to create stable compact phenotypes. These approaches have been pursued in several ornamental plants, e.g., Calibrachoa (Gennarelli et al, 2009), Kalanchoë (Christensen et al, 2008), Mecardonia (Pérez de la Torre et al, 2018), Petunia (Mishiba et al, 2006), and poinsettia (Islam et al, 2013). Some of these strategies target alteration of gibberellic acid (GA) metabolism; GA 20 oxidases have silenced (Topp et al, 2008), GA 2 oxidases have been over-expressed (Gargul et al, 2013), the Arabidopsis thaliana Short Internodes gene has been overexpressed (Lütken et al, 2010;Islam et al, 2013), and knotted homeobox genes have been modulated (Lütken et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Transgenic Kalanchoë blossfeldiana, Containing Individual rol Genes and Open Reading Frames Under 35S Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers

et al. 2021

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Reduced growth habit is a desirable trait for ornamental potted plants and can successfully be obtained through Rhizobium rhizogenes transformation in a stable and heritable manner. Additionally, it can also be obtained by transformation with Agrobacterium tumefaciens harboring specific genes from R. rhizogenes. The bacterial T-DNA harbors four root oncogenic loci (rol) genes and 14 less known open reading frames (ORFs). The four rol genes, i.e., rolA, rolB, rolC, and rolD, are conceived as the common denominator for the compact phenotype and the other less characterized ORFs seem auxiliary but present a potential breeding target for less aberrant and/or more tailored phenotypes. In this study, Kalanchoë blossfeldiana ‘Molly’ was transformed with individual rol genes and selected ORFs in 35S overexpressing cassettes to comprehensively characterize growth traits, gene copy and expression, and ethylene tolerance of the flowers. An association of reduced growth habit, e.g. height and diameter, was observed for rolB2 and ORF14-2 when a transgene single copy and high gene expression were detected. Chlorophyll content was reduced in overexpressing lines compared to wild type (WT), except for one ΔORF13a (a truncated ORF13a, where SPXX DNA-binding motif is absent). The flower number severely decreased in the overexpressing lines compared to WT. The anthesis timing showed that WT opened the first flower at 68.9 ± 0.9 days and the overexpressing lines showed similar or up to 24 days delay in flowering. In general, a single or low relative gene copy insertion was correlated to higher gene expression, ca. 3 to 5-fold, in rolB and ΔORF13a lines, while in ORF14 such relation was not directly linked. The increased gene expression observed in rolB2 and ΔORF13a-2 contributed to reducing plant growth and a more compact habit. Tolerance of detached flowers to 0.5 μl L−1 ethylene was markedly higher for ORF14 with 66% less flower closure at day 3 compared to WT. The subcellular localization of rolC and ΔORF13a was investigated by transient expression in Nicotiana benthamiana and confocal images showed that rolC and ΔORF13a are soluble and localize in the cytoplasm being able to enter the nucleus.

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Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits

Cited by 12 publications

References 82 publications

Transcription Factors Associated with Leaf Senescence in Crops

Transcription Factors Associated with Leaf Senescence in Crops

Challenges and potentials of new breeding techniques in Cannabis sativa

Transgenic Kalanchoë blossfeldiana, Containing Individual rol Genes and Open Reading Frames Under 35S Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers

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