Transformation of lignocellulose to starch‐like carbohydrates by organic acid‐catalyzed pretreatment and biological detoxification

Zhang, Bin; Khushik, Faryal A; Zhan, Baorui; Bao, Jie

doi:10.1002/bit.27887

Cited by 23 publications

(15 citation statements)

References 40 publications

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“…[ 43 ] Interestingly, current literature does not indicate the presence of oxalic acid in the exudate of P. variotii ; on the contrary, this fungi has been shown to degrade hydrocarbons including oxalic acid. [ 44 ] Further, P. variotii has been reported to contain a widely diverse set of organic acids in its exudate, primarily fatty acids. [ 32 ] This diversity in the organic acid profiles for P. chrysogenum and P. variotii may have an impact on the presence of multiple ZnO particle morphologies within the same preparation.…”

Section: Resultsmentioning

confidence: 99%

Mycosynthesis of Zinc Oxide Nanoparticles Exhibits Fungal Species Dependent Morphological Preference

Brady

O'Leary

Moormann

et al. 2023

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Filamentous fungi can synthesize a variety of nanoparticles (NPs), a process referred to as mycosynthesis that requires little energy input, do not require the use of harsh chemicals, occurs at near neutral pH, and do not produce toxic byproducts. While NP synthesis involves reactions between metal ions and exudates produced by the fungi, the chemical and biochemical parameters underlying this process remain poorly understood. Here, the role of fungal species and precursor salt on the mycosynthesis of zinc oxide (ZnO) NPs is investigated. This data demonstrates that all five fungal species tested are able to produce ZnO structures that can be morphologically classified into i) well‐defined NPs, ii) coalesced/dissolving NPs, and iii) micron‐sized square plates. Further, species‐dependent preferences for these morphologies are observed, suggesting potential differences in the profile or concentration of the biochemical constituents in their individual exudates. This data also demonstrates that mycosynthesis of ZnO NPs is independent of the anion species, with nitrate, sulfate, and chloride showing no effect on NP production. These results enhance the understanding of factors controlling the mycosynthesis of ceramic NPs, supporting future studies that can enable control over the physical and chemical properties of NPs formed through this “green” synthesis method.

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Section: Resultsmentioning

confidence: 99%

Mycosynthesis of Zinc Oxide Nanoparticles Exhibits Fungal Species Dependent Morphological Preference

Brady

O'Leary

Moormann

et al. 2023

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“…The raw biomass was air dried and milled, and then pretreated using the dry acid pretreatment method [ 32 , 33 ]. Acid pretreatment was operated according to the protocols in [ 34 – 38 ]. A 20-L helical ribbon impeller-driven reactor was fed with 1200 g of corn stover (dry base) and 500–600 g of sulfuric acid solution to the dry solid weight to the acid liquid weight of 2:1.…”

Section: Methodsmentioning

confidence: 99%

Cellulosic hydrocarbons production by engineering dual synthesis pathways in Corynebacterium glutamicum

Hua

Huang

et al. 2022

Biotechnol Biofuels

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Background Lignocellulose provides the only practical carbohydrates feedstock for sustainable bioproduction of hydrocarbons as future alternative of fossil fuels. Production of hydrocarbons from lignocellulose is achieved by a biorefinery process chain including pretreatment to breakdown the crystalline structure for cellulase-catalyzed hydrolysis, detoxification of inhibitory compounds generated during pretreatment, enzymatic hydrolysis to fermentable monosaccharide sugars, and fermentation to hydrocarbon products. The major barriers on fermentative production of hydrocarbons from lignocellulose include two aspects: one is the inherent stress of pretreatment-derived inhibitors on microbial cells, the other is the toxicity of hydrocarbons to cell membranes. The microbial cell factory should be tolerant to both inhibitor stress and hydrocarbons toxicity. Results Corynebacterium glutamicum was selected as the starting strain of hydrocarbons synthesis since it is well adapted to lignocellulose hydrolysate environment. The dual hydrocarbon synthesis pathways were constructed in an industrial C. glutamicum S9114 strain. The first pathway was the regular one in microalgae composed of fatty acyl-acyl carrier protein (fatty acyl-ACP) reductase (AAR) and aldehyde deformylating oxygenase (ADO) with fatty acyl-ACP as precursor. The second pathway was the direct decarboxylation of free fatty acid by fatty acid decarboxylase (OleT) using the rich fatty acids from the disruption of the transcriptional regulator fasR gene. The transmembrane transportation of hydrocarbon products was avoided by secretively expressing the fatty acid decarboxylase (OleT) to the extracellular space. The hydrocarbons generation from glucose reached 29.2 mg/L, in which the direct decarboxylation pathway contributed more than 70% of the total hydrocarbons generation, and the AAR–ADO pathway contributed the rest 30%. Conclusion The dual hydrocarbon synthesis pathways (OleT and AAR–ADO pathways) were constructed in the inhibitors tolerant C. glutamicum S9114 strain for hydrocarbon production using lignocellulose feedstock as the starting feedstock. When corn stover was used for hydrocarbons production after dry acid pretreatment and biodetoxification, the hydrocarbons generation reached 16.0 mg/L. This study provided a new strategy for hydrocarbons synthesis using microbial cell factory suitable for lignocellulose feedstock.

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“…In the present study, L-lactic acid was produced by applying a new biorefining approach of dry-acid pretreatment and biodetoxification into "starch-like" carbohydrates from lignocellulose. This can be said to be similar to dry milled corn meal, but with no wastewater discharge, a low energy consumption, and minimum sugar loss (Liu et al, 2018;B. Zhang et al, 2021).…”

Section: Design Considerations For Cyclic L-lactide Productionmentioning

confidence: 99%

“…In the present study, l ‐lactic acid was produced by applying a new biorefining approach of dry‐acid pretreatment and biodetoxification into “starch‐like” carbohydrates from lignocellulose. This can be said to be similar to dry milled corn meal, but with no wastewater discharge, a low energy consumption, and minimum sugar loss (Liu et al, 2018; B. Zhang et al, 2021). The inhibitors accumulated in the pretreated lignocellulose solids were effectively removed by biodetoxification, in which furfural, HMF, organic acids, and partially phenolics were converted into CO 2 and water, before bioconversion (Yi et al, 2019).…”

Section: Purification Of Cellulosic L‐lactic Acid and Its Characteriz...mentioning

confidence: 99%

Cyclic l‐lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation

Jia

Qiu

et al. 2022

Biotech & Bioengineering

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Cyclic chiral lactide is the monomer chemical for polymerization of high molecular weight polylactic acid (PLA). The synthesis of cyclic l‐lactide starts from poly‐condensation of l‐lactic acid to a low molecular weight prepolymer and then depolymerized to cyclic l‐lactide. Lignocellulose biomass is the most promising carbohydrate feedstock for lactic acid production, but the synthesis of cyclic l‐lactide from l‐lactic acid produced from lignocellulose has so far not been successful. The major barriers are the impurities of residual sugars and inhibitors in the crude cellulosic l‐lactic acid product. Here we show a successful cyclic l‐lactide synthesis from cellulosic l‐lactic acid by lignocellulose biorefining with complete inhibitor removal and coordinated sugars assimilation. The removal of inhibitors from lignocellulose pretreatment was accomplished by biodetoxification using a unique fungus Amorphotheca resinae ZN1. The nonglucose sugars were completely and simultaneously assimilated at the same rate with glucose by the engineered l‐lactic acid bacterium Pediococcus acidilactici. The l‐lactic acid production from wheat straw was comparable to that from corn starch with high optical pure (99.6%), high l‐lactic acid titer (129.4 g/L), minor residual total sugars (~2.2 g/L), and inhibitors free. The cyclic l‐lactide was successfully synthesized from the regularly purified l‐lactic acid and verified by detailed characterizations. This study paves the technical foundation of carbon‐neutral production of biodegradable PLA from lignocellulose biomass.

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Transformation of lignocellulose to starch‐like carbohydrates by organic acid‐catalyzed pretreatment and biological detoxification

Cited by 23 publications

References 40 publications

Mycosynthesis of Zinc Oxide Nanoparticles Exhibits Fungal Species Dependent Morphological Preference

Mycosynthesis of Zinc Oxide Nanoparticles Exhibits Fungal Species Dependent Morphological Preference

Cellulosic hydrocarbons production by engineering dual synthesis pathways in Corynebacterium glutamicum

Cyclic l‐lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation

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