Transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus

Krastanova, S.; Perrin, Mireille; Barbier, Pascale; Demangeat, Gérard; Cornuet, P; Bardonnet, Nicolas; Otten, Léon; Pinck, L.; Walter, Bernard

doi:10.1007/bf00231936

Cited by 74 publications

(30 citation statements)

References 12 publications

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“…Stressing the need for improving the quality and quantity of in vitro-grown grapevine plants, Mullins et al (1990) reported a successful genetic transformation protocol for V. rupestris L. through somatic embryogenesis. Some important agronomic traits have consequently been transferred into the grape (V. vinifera) genome by transformation of somatic embryos derived from unfertilised ovules (Yamamoto et al 2000), zygotic embryos (Scorza et al 1995) and hypocotyl explants (Mullins et al 1990;Krastanova et al 1995). The limitation of the methods applied is the brief period that anthers, ovules and zygotic embryos are available.…”

Section: Introductionmentioning

confidence: 99%

An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.)

et al. 2002

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By manipulating hormone levels, light intensities and temperature, we have developed an efficient leaf-disc method for the regeneration of plants via embryogenesis and for transformation in four genotypes of Vitis vinifera L. In MS basal medium supplemented with 1 mg l -1 6-benzylaminopurine (BAP) and 0.1 mg l -1 2,4-dichlorophenoxyacetic acid, leaf discs cultured for 2 weeks under dark conditions produced calli in over 80% of the cultures. These subsequently differentiated into pro-embryos and embryos only if kept under conditions of low light intensity (15 µE m -2 s -1 ) for 2 weeks before being transferred to conditions of high light intensity (60 µE m -2 s -1 ). If the calli were directly transferred to high light intensity, the differentiation into embryos was blocked and the calli turned pink. The somatic embryos germinated at a frequency of about 10% on NN basal medium and about 32% on NN medium supplemented with 1 mg l -1 BAP and 0.1 mg l -1 indole-3-butyric acid. The embryos, however, germinated when pre-exposed to a low temperature of 4°C for 2 weeks. If they were transferred directly to room temperature under conditions of high light intensity (60 µE m -2 s -1 ), shoot buds were produced, whereas under conditions of low light intensity (15 µE m -2 s -1 ) secondary embryogenesis was induced. About 90-95% of the in vitro grown plantlets could be successfully transferred to soil. The above method was also applicable for developing transgenic embryos whose transgenic nature was monitored using β-glucuronidase as a reporter gene.

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Section: Introductionmentioning

confidence: 99%

An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.)

et al. 2002

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“…Transgenic plants of several nematode-susceptible grape rootstocks have been obtained, that produced the GCMV (Le Gall et al, 1994) and GFLV (Krastanova et al, 1995) coat proteins.…”

Section: Introductionmentioning

confidence: 99%

Adventitious bud formation and shoot development from in vitro leaves of Vitis x Muscadinia hybrids

Torregrosa¹,

Bouquet²

1996

Plant Cell Tiss Organ Cult

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High levels of regeneration were obtained from young leaves excised from axillary shoots in proliferating nodal cultures of several Vitis x Muscadinia hybrids. Best results were obtained when the explants were cultured on solidified half-strength Murashige and Skoog medium supplemented with 8.9/~M 6-benzyladenine and 0.05 #M 1-naphthaleneacetic acid. Though variation was observed among the hybrids, the procedure used does not seem to be genotype-specific as all the hybrids and cultivars tested could regenerate. Scanning electron microscopy observations and histological studies carried out during the development of adventitious shoot organogenesis revealed that the promeristem initiation occurred in the outer cell layers near the wounded area of the petiolar stub.Abbreviations: BA -6-benzyladenine; NAA -1-naphthaleneacetic acid

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“…Biotechnology arises as an alternative to allow the generation of virus-resistant grapevine plants by transgenesis, mainly involving the expression of viral components and exploiting the naturally occurring gene silencing [107][108][109][110][111][112][113][114][115][116][117]. This strategy requires plant transformation with a short sequence of the pathogen genome in a way that a double-strand RNA structure is formed during transcription, initiating gene silencing in the host.…”

Section: Inducing Virus Resistance In Grapevine By Transgenesismentioning

confidence: 99%

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Armijo¹,

Espinoza²,

Loyola³

et al. 2016

Grape and Wine Biotechnology

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Grapevine is one of the most abundant crops worldwide, with varieties destined for fresh and dry consumption, as well as wine production. Unfortunately, grapevine plants are affected by both biotic and abiotic stresses, generating significant economic losses. These conditions can negatively impact grape cultivation at different stages: plant and berry development during pre-and post-harvest, production, fresh fruit processing and export, along with wine quality. Most of the grapevine varieties are susceptible to several pathogens and within this chapter, particular attention is given to fungi (Botrytis cinerea and Erysiphe necator) and viruses, since they are a worldwide concern. Within the latter, special focus is given to the grapevine leafroll disease, a complex and destructive infection. On the other hand, abiotic stress is also relevant in grapevine, and in this chapter it will be exemplified by UV-B radiation and its impact on growth and fruit development, plant adaptive responses and its relationship with the quality of grape berries for winemaking. The main biotic and abiotic grapevine stress factors are reviewed in this chapter, considering a special focus on biotechnological approaches carried out in order to address them and minimize their detrimental consequences.

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Transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus

Cited by 74 publications

References 12 publications

An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.)

An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.)

Adventitious bud formation and shoot development from in vitro leaves of Vitis x Muscadinia hybrids

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

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