Transformation of diclofenac by the indigenous microflora of river sediments and identification of a major intermediate

Gröning, Janosch A. D.; Held, Claudia; Garten, Claudia; Claußnitzer, Ute; Kaschabek, Stefan R.; Schlömann, Michael

doi:10.1016/j.chemosphere.2007.03.037

Cited by 120 publications

(57 citation statements)

References 18 publications

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“…Diclofenac is discharged with treated wastewater to surface water where it can undergo accumulation in sediments [96]. Biodegradation of diclofenac can last up to several months [5].…”

Section: Ecotoxicity Of Pharmaceuticalsmentioning

confidence: 99%

Fate and Removal of Pharmaceuticals and Illegal Drugs Present in Drinking Water and Wastewater

Szymonik

Lach

Malińska

2017

Ecological Chemistry and Engineering S

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Rapid development of pharmaceutical industry, and thus widespread availability of different types of therapeutical and increased intake of pharmaceuticals, results in elevated concentrations of pharmaceuticals in municipal wastewater subjected to treatment in wastewater treatment plants. Pharmaceuticals present in raw wastewater discharged from hospitals, households, veterinary and health care clinics eventually end up in wastewater treatment plants. Commonly applied methods for treating wastewater do not allow complete removal of these contaminants. As a consequence, pharmaceuticals still present in treated wastewater are introduced to water environment. The most frequently identified pharmaceuticals in surface water belong to the following groups: non steroidal anti inflammatory drugs, beta-blockers, estrogens and lipid regulators. The most difficult is removal of diclofenac, clofibric acid and carbamazepine as these substances show low biodegradability. Diclofenac can be removed in the process of wastewater treatment by 40%, carbamazepine by 10%, and clofibric acid from 26 to 50%. The presence of diclofenac sodium in the rivers in Poland was confirmed and the concentrations were following: 380 ng/dm 3 (the Warta river), 470 ng/dm 3 (the Odra river), 140 ng/dm 3 (the Vistula river). Naproxene was found in the Warta river at the concentration of 100 ng/dm 3 . The presence of pharmaceuticals in surface water can be toxic to aqueous microorganisms and fish. Recent studies confirmed also the presence of pharmaceuticals in drinking water. This is considered as a problem especially in urban agglomerations such as Berlin or large cities in Spain and China. The studies showed that pharmaceuticals were also identified in the samples taken from the Polish rivers and drinking water. The presence of naproxene and diclofenac at the concentrations of 13 and 4 ng/dm 3 was identified in drinking water sampled from water intakes in Poznan. Surface water and drinking water showed also the presence of illegal drugs.

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“…Diclofenac is discharged with treated wastewater to surface water where it can undergo accumulation in sediments [96]. Biodegradation of diclofenac can last up to several months [5].…”

Section: Ecotoxicity Of Pharmaceuticalsmentioning

confidence: 99%

Fate and Removal of Pharmaceuticals and Illegal Drugs Present in Drinking Water and Wastewater

Szymonik

Lach

Malińska

2017

Ecological Chemistry and Engineering S

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“…Enzymes were not indicated, but it was suggested that p-benzoquinone imine was formed out of 5-hydroxydiclofenac by dehydratation (Fig. 2) (Groning et al 2007). …”

Section: Microbial Degradation/transformation Of Diclofenacmentioning

confidence: 99%

“…IMI35348 transformed diclofenac to 5-hydroxydiclofenac and 4 0 -hydroxydiclofenac (Webster et al 1998;Vail et al 2005). Groning et al (2007) investigated diclofenac degradation in river sediment. The authors assayed intermediates of diclofenac: 4 0 -hydroxydiclofenac, 5-hydroxydiclofenac and the major metabolite of 5-hydroxydiclofenac-pbenzoquinone imine.…”

Section: Microbial Degradation/transformation Of Diclofenacmentioning

confidence: 99%

Biodegradation and biotransformation of polycyclic non-steroidal anti-inflammatory drugs

Domaradzka

Guzik

Wojcieszyńska

2015

Rev Environ Sci Biotechnol

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In recent years the increased use of polycyclic non-steroidal anti-inflammatory drugs has resulted in their presence in the environment. This in turn may cause potential negative effects on living organisms. While the biotransformation mechanisms of polycyclic non-steroidal anti-inflammatory drugs in the human body and in other mammals have been extensively studied, degradation of these drugs by microorganisms has seldom been investigated and is largely unknown. Biotransformation/biodegradation of polycyclic nonsteroidal anti-inflammatory drugs is caused by fungal microorganisms, mainly white-rot fungi, and a few strains of bacteria. However, hitherto only complete degradation of olsazine was described. The first step of the transformation is most often hydroxylation catalyzed by cytochrom P-450 monooxygenases, or oxygenation by laccases and three peroxidases: lignin peroxidase, manganese-dependent peroxidase and versatile peroxidase manganese-dependent peroxidase. The aim of this work is to summarize the knowledge about the biotransformation and/or biodegradation of polycyclic non-steroidal anti-inflammatory drugs and to present their biotransformation pathways.

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“…Despite the existing reports on the microbial degradation of DCF, there are still knowledge gaps related to the degradation process. Gröning et al (2007) observed the removal of DCF in a concentration of 3-35 µM in a river sediment, with transient accumulation of a major metabolite, the pbenzoquinone imine derivative of 5-hydroxy-DCF, which is further abiotically adsorbed on the biofilm and this reveals a lack of biodegradation potential for this intermediate compound. Moreira et al (2018) reported that the bacterial strain L. portucalensis F11 degraded 70% of 34 μM of DCF, supplied as the sole carbon source after 30 days of the culture; while its complete degradation was achieved via co-metabolism with acetate after 25 days.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

Węgrzyn

Felis

2018

Polish Journal of Microbiology

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A b s t r a c t Diclofenac (DCF), a non-steroidal anti-inflammatory drug (NSAID) and sulfamethoxazole (SMX), an antimicrobial agent, are in common use and can be often detected in the environment. The constructed wetland systems (CWs) are one of the technologies to remove them from the aquatic environment. The final effect of the treatment processes depends on many factors, including the interaction between plants and the plant-associated microorganisms present in the system. Bacteria living inside the plant as endophytes are exposed to secondary metabolites in the tissues. Therefore, they can possess the potential to degrade aromatic structures, including residues of pharmaceuticals. The endophytic strain MG7 identified as Microbacterium sp., obtained from root tissues of Phalaris arundinacea exposed to DCF and SMX was tested for the ability to remove 2 mg/l of SMX and DCF in monosubstrate cultures and in the presence of phenol as an additional carbon source. The MG7 strain was able to remove approximately 15% of DCF and 9% of SMX after 20 days of monosubstrate culture. However, a decrease in the optical density of the MG7 strain cultures was observed, caused by an insufficient carbon source for bacterial growth and proliferation. The adsorption of pharmaceuticals onto autoclaved cells was negligible, which confirmed that the tested strain was directly involved in the removal of DCF and SMX. In the presence of phenol as the additional carbon source, the MG7 strain was able to remove approximately 35% of DCF and 61% of SMX, while an increase in the optical density of the cultures was noted. The higher removal efficiency can be explained by adaptive mechanisms in microorganisms exposed to phenol (i.e. changes in the composition of membrane lipids) and by a co-metabolic mechanism, where non-growth substrates can be transformed by non-specific enzymes. The presence of both DCF and SMX and the influence of the supply frequency of CWs with the contaminated wastewater on the diversity of whole endophytic bacterial communities were demonstrated. The results of this study suggest the capability of the MG7 strain to degrade DCF and SMX. This finding deserves further investigations to improve wastewater treatment in CWs with the possible use of pharmaceuticals-degrading endophytes.K e y w o r d s: endophytic bacteria, constructed wetlands, diclofenac, sulfamethoxazole, biodegradation Węgrzyn A. and Felis E.

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Transformation of diclofenac by the indigenous microflora of river sediments and identification of a major intermediate

Cited by 120 publications

References 18 publications

Fate and Removal of Pharmaceuticals and Illegal Drugs Present in Drinking Water and Wastewater

Fate and Removal of Pharmaceuticals and Illegal Drugs Present in Drinking Water and Wastewater

Biodegradation and biotransformation of polycyclic non-steroidal anti-inflammatory drugs

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

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