Transformation of cucumber (Cucumis sativus L.) plants with Agrobacterium rhizogenes

Trulson, A. J.; Simpson, Robert B.; Shahin, Elias A.

doi:10.1007/bf00273711

Cited by 117 publications

(42 citation statements)

References 28 publications

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“…To date, transgenic plants of C. sativus (Truson, Simpson, and Shahin 1986;Chee 1990), C. melo (Guis et al 1998), Cucurbita maxima and C. pepo (Pang et al 2000), and C. lanatus (Choi, Soh, and Kim 1994) were released. Various methodologies, including Agrobacterium rhizagenes and A. tumefaciensmediated transformation via somatic embryogenesis and organogenesis and biolistic transformation, have been used for production of these plants.…”

Section: Discussionmentioning

confidence: 99%

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Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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Section: Discussionmentioning

confidence: 99%

“…Transgenic cucumber plants were first produced by Agrobacterium rhiwgenes-mediated transformation via somatic embryogenesis (Truson, Simpson, and Shahin 1986). Subsequent reports of the production of transgenic cucumbers mediated by A. tumefaciens via somatic embryogenesis include those by Chee (1990) and Tabei et al (1994).…”

Section: Cucumber (Cucumis Sativus)mentioning

confidence: 99%

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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“…While 50% of the plant clones regenerated (rolA + /rolB + /rolC + ) exhibited the typical hairy root syndrome, the remaining 50% (rolA − /rolB − /rolC − ) resembled the nontransformed plants, with a few altered characteristics [12,13]. The loss of the rolA, rolB, and rolC genes in the latter 50% of the clones in H. muticus could be due to the deletion of the rol genes during protoplast isolation or regeneration [11,50,51]. Hänisch ten Cate et al [11] concluded from their study in Solanum tuberosum that, during long-term hairy root culture and regeneration, deletions of TL-DNA and TR-DNA can occur.…”

Section: Discussionmentioning

confidence: 99%

The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

2015

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The fate of integrated Ri T-DNA rol genes during regeneration via indirect somatic embryogenesis and stability of its effect on morphology and tylophorine content of Ri-transformed plants have been studied in Tylophora indica. Integration and expression of Ri T-DNA genes in transformed embryogenic callus lines derived from transformed root lines, 300 Ri-transformed somatic embryos, and 23 Ri-transformed plant lines were analysed. Fifty root lines studied showed integration and expression of four rol genes of TL-DNA. Spontaneous regeneration via indirect somatic embryogenesis was obtained from root lines that were TL + /TR − . Stable integration and expression of rol genes were observed in root lines, embryogenic callus lines, and the spontaneously induced somatic embryos. Nineteen out of the 23 Ri-transformed plant lines and their clones showed phenotypic and genetic stability over the period of 3 years. Four Ri-transformed plants were morphologically similar to nontransformed plants but showed variation with the integration and expression of the rolA gene and absence of other rol genes. Variant Ri-transformed plant line A 4 28#1-V showed highest tylophorine content (2.93 ± 0.03 mg gDW −1 ) among plant lines studied. The effects of T-DNA genes on growth, morphology, and tylophorine content of the Ri-transformed plants were stable in the long term culture.

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“…The adventitious roots named as hairy roots grow vigorously in phytohormone-free medium and provide a useful material for studies on secondary metabolite production (9,10). In a number of plant species, plant regeneration from hairy roots could be observed when they were cultured on medium with phytohormones (19). On the other hand, in some species, hairy roots cultured under light and/or dark conditions formed adventitious shoots on phytohormone-free medium (5,12,18).…”

Section: Induction and Maintenance Of Hairy Rootsmentioning

confidence: 99%

Light Requirement for Shoot Regeneration in Horseradish Hairy Roots

Saitou

Kamada²,

Harada³

1992

Plant Physiol.

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Hairy roots of horseradish (Armoracia rusticana) were induced by inoculation with Agrobacterium rhizogenes harboring Ri plasmid and cultured on phytohormone-free Murashige and Skoog medium after eliminating the bacteria. Hairy roots grew vigorously and sometimes formed yellowish calli under dark conditions. On the other hand, growth of hairy roots stopped after several weeks of culture with light, then shoots were regenerated. Frequency of shoot formation from hairy roots increased as the culture period in light lengthened and the light intensity increased. The shoot regeneration was induced by treatment with white or red light, but not with far-red light. Shoot regeneration by red light was inhibited by following treatment with far-red light. Red and far-red light reversibly affected shoot regeneration. Excised roots of nontransformed plants grew quite slowly on phytohormone-free Murashige and Skoog medium and occasionally formed shoots under white light conditions. ment for shoot formation in both hairy roots and excised roots of nontransformed plants of horseradish. MATERIALS AND METHODS Induction and Maintenance of Hairy RootsHairy roots were obtained by inoculating sterilized leaves of horseradish (Armoracia rusticana) with Agrobacterium rhizogenes strain 15834 harboring Ri plasmid (pRi 15834). Several weeks after the inoculation, tip segments of hairy roots that appeared on the inoculated sites were cut off and cultured on hormone-free MS2 semi-solidified (0.2% gerlite) medium containing an antibiotic (Claforan, 500 mg/L) at 250C under dark conditions. Segments of growing hairy roots were transferred several times to the fresh MS medium to eliminate the bacteria and then transferred to hormone-free MS medium without antibiotics.

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Transformation of cucumber (Cucumis sativus L.) plants with Agrobacterium rhizogenes

Cited by 117 publications

References 28 publications

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

Light Requirement for Shoot Regeneration in Horseradish Hairy Roots

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