Transformation of Bacillus thuringiensis protoplasts by plasmid deoxyribonucleic acid

Martin, Phyllis A. W.; Lohr, J R; Dean, Donald H.

doi:10.1128/jb.145.2.980-983.1981

Cited by 77 publications

(19 citation statements)

References 20 publications

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“…Transformation with pBC16 occurred at a usual frequency of 10-5 to 10-6 of the recipient protoplasts, which corresponds to 10-3 to 10-4 of the regenerated protoplasts. Similar (or lower) transformation frequencies have been reported for B. amyloliquefaciens (17), B. thuringiensis (1,26), and other strains of B. megaterium (3,34).…”

Section: Discussionsupporting

confidence: 85%

Megacinogenic plasmids of Bacillus megaterium

Tersch¹,

Carlton²

1983

J Bacteriol

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Megacins A-216 and A-19213 in Bacillus megaterium are plasmid encoded, as shown by analysis of cured, non-megacinogenic (Meg-) derivatives of strains 216 and ATCC 19213 and by polyethylene glycol-mediated protoplast transformation of Megbacteria with plasmid DNA. The results of both techniques implicated a 31-megadalton plasmid, pBM309, in megacin A-216 production and a 29-megadalton plasmid, pBM113, in megacin A-19213 production.

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Section: Discussionsupporting

confidence: 85%

Megacinogenic plasmids of Bacillus megaterium

Tersch¹,

Carlton²

1983

J Bacteriol

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“…The indigenous bacterial communities capable of growing on 5 mM Naphthalene were serially diluted with sterile saline and plated on the surfaces of L.B agar plates (by surface technique) (Martin, Lohr, & Dean, 1981).…”

Section: Isolation Of Different Bacterial Strains Capable Of Growing mentioning

confidence: 99%

Biodegradation of naphthalene by Bordetella avium isolated from petroleum refinery wastewater in Egypt and its pathway

Abo-State

Riad

Bakr

et al. 2018

Journal of Radiation Research and Applied Sciences

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a b s t r a c tWastewater and Sludge samples polluted with petroleum oil from Cairo Oil Refining Company (CORC), Mostorod, El-Qalyubiah, Cairo, Egypt. were used for isolation of indigenous bacterial communities. The isolation of bacterial strains followed four steps of adaptation and enrichment technique for selection of the most Naphthalene tolerant bacterial strains. Screening on four Naphthalene concentrations to determine the most potent strains having the abilities to use Naphthalene as a sole carbon and energy source have been conducted. The most potent bacterial isolates were MAM-P9, MAM-P14, MAM-P22, MAM-P25 and MAM-P26. The abilities of the five most potent bacterial isolates to grow on BSM amended with 4,5,6 and 7 mM Naphthalene have been determined by recording their growth (O.D) and secretion of extracellular protein after zero time (initial), 1,2,3,4,5,6,7,14 and 21 days spectrophotometrically.The degradation of Naph. have been determined quantitatively by High Performance Liquid Chromatography (HPLC) and qualitatively by GC/MS to determine the Intermediates resulted from degradation.The results revealed that isolate MAM-P22 was the best Naphthalene degrader. It degraded 95% of the highest concentration 7 mM. The best Naphthalene degrader bacterial Isolate MAM-22 was identified by 16S-rRNA showed a similarity of 98% to Bordetella avium strain with accession No. 041769.1. The results of GC/MS analysis revealed that Bordetella avium MAM-P22 degraded Naphthalene to give six intermediate compounds, These compounds were 1,2-Benzene dicarboxylic acid, Butyl-2,4-dimethyl-2-nitro-4pentenoate, 1-Nonen-3-ol, Eicosane, Nonacosane.

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“…All rock samples have been collected from the Gabel El Sela area, which is located about 20 km west of Abu Ramad Town as indicated in Figure 1. It is bound by latitudes 22 [13][14][15][16][17][18][19][20][21][22] 20N and longitudes 36 08-36 17E. This area covers about 185 km 2 and is occupied mainly by different Precambrian basement rocks of the South Eastern Desert of Egypt.…”

Section: Sampling Sitementioning

confidence: 99%

“…From the appropriate three successive dilutions, 100 ll were inoculated and spread on the surface of L.B. agar plates [16].…”

Section: Isolation Of Bacterial Strainsmentioning

confidence: 99%

Characterization, kinetics and thermodynamics of biosynthesized uranium nanoparticles (UNPs)

Abo-State

Saleh

Mira

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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The present study was carried out to explore the potential of the isolated bacterial strains isolated from Gabal El Sela in Eastern Dessert, Egypt for biosynthesis of uraninite nanoparticles intracellularly. The most potent bacterial strains associated (intra) with uranium nanoparticles were characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) and Energy Dispersive X-ray (EDX). Studying factors affecting biosynthesis of uranium nanoparticles indicated that the optimum conditions were 6000 ppm uranium concentrations at pH 7.0 and temperature 30 °C ± 1 after five days with 10% biomass under shaking conditions and the maximum uranium uptake by MAM - U9 cells was 3300 ppm (55%) from uranyl nitrate solution and 3600 ppm (72%) from Sela rock sample. Results of TEM micrograph show those uranium nanoparticles (UNPs) with size ranging from 2.9 to 21.13 nm inside cells. The kinetics, isotherm and thermodynamics parameters of uranium uptaken by bacterial strain MAM -U9 have been determined and found to be a first order process (R = 9935), follows Langmuir isotherm (R = 0.998) and the thermodynamics of ΔG = -9.715 kJmol, ΔH = 16.987 kJmol and ΔS = 0.0881 kJmolK at 30 °C.

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Transformation of Bacillus thuringiensis protoplasts by plasmid deoxyribonucleic acid

Cited by 77 publications

References 20 publications

Megacinogenic plasmids of Bacillus megaterium

Megacinogenic plasmids of Bacillus megaterium

Biodegradation of naphthalene by Bordetella avium isolated from petroleum refinery wastewater in Egypt and its pathway

Characterization, kinetics and thermodynamics of biosynthesized uranium nanoparticles (UNPs)

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