Abstract:Nicotiana tabacum protoplasts were transformed by polyethylene glycol (PEG)‐mediated uptake and electroporation, with circular and linear DNA, and with or without X‐ray irradiation. We investigated the influence on the transient expression by these parameters as well as on the frequencies for stable transformation. Plants were regenerated and selfed, and the progenies of the transformed plants were analysed and used to compare the pattern of gene integration by these different variations in transformation meth… Show more
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