Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products.

Pillemer, E; Whitlock, Cheryl A.; Weissman, Irving L.

doi:10.1073/pnas.81.14.4434

Cited by 49 publications

(20 citation statements)

References 26 publications

(20 reference statements)

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“…These results support by tumor cells is confined to age (9) . Additionally, we present evidence here that the 6C3 antigen is expressed in early B leukemias that have been induced with a variety of "cytoplasmic", oncogenes but never with the "nuclear" myc oncogene .…”

Section: Discussionsupporting

confidence: 59%

Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc.

Morse¹,

Tidmarsh²,

Holmes³

et al. 1987

The Journal of Experimental Medicine

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The monoclonal antibody 6C3 was used to test a wide variety of murine hematopoietic neoplasms for cell surface expression of a 160 kD glycoprotein (gp160(6C3)) previously shown to be expressed by neoplastic pre-B and some B lymphocytes transformed by Abelson murine leukemia virus (A-MuLV). This antigen was expressed on many pre-B and B cell lymphomas, but not on A-MuLV-transformed fibroblasts, T cell lymphomas, or myelomonocytic leukemias, gp160(6C3) was expressed by most early B-lineage spontaneous tumors, and early B tumors induced by replication-defective MuLV-containing oncogenes the products of which are associated with the cytoplasmic aspect of the plasma membrane, i.e., fes, abl, H-ras, bas, src, erbB, and Cas NS-1. By comparison, none of the early B lineage lymphomas induced by the "nuclear" oncogene avian v-myc MuLV, or arising in mice transgenic for a murine c-myc gene, or later B cell lineage stages bearing translocations of the c-myc locus expressed this antigen.

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Section: Discussionsupporting

confidence: 59%

Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc.

Morse¹,

Tidmarsh²,

Holmes³

et al. 1987

The Journal of Experimental Medicine

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“…1B). This finding is particularly interesting because the glycoprotein identified by BP-1 antibody is identical to that bound by the 6C3 antibody ( [38] and Q. Wu, G . Tidmarsh, I. Weissman and M. Cooper, manuscript in preparation), and the 6C3 antigen is present on some lymphocyte-supporting stromal cell lines [39].…”

Section: Characterization Of Adherent Cellsmentioning

confidence: 91%

Relationships between B‐lineage lymphocytes and stromal cells in long‐term bone marrow cultures

et al. 1987

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In long-term culture of mouse bone marrow, the growth and differentiation of B-lineage lymphocytes depends on interaction with adherent cells or their products. The objectives of these studies were to characterize the types of cells present in the supporting adherent layer as well as the physical relationships of these cells with lymphocytes. With an extensive panel of antibodies against hemopoietic and lymphocyte antigens, two discrete nonlymphoid populations were identified: macrophages and undefined, large cells which we termed "stromal cells". Lymphocyte clusters grew in actual contact with the latter cells only. Stromal cells lacked expression of most hemopoietic antigens, including the common leukocyte antigen, J11d, heat stable antigen (M1/69), Thy-1 and BP 1. Antigens expressed by stromal cells were detected by AA4.1, our 94.2 antibody, and antibody to the Forsmann antigen, but the most distinguishing characteristics of the lymphocyte-binding stromal cells were production of basement membrane components, laminin and collagen IV, and the extremely low uptake of acetylated low density lipoprotein (LDL). Using acetylated LDL uptake as a sorting criterion, the lymphocyte-binding stromal cells were separated from the macrophages, recultured and shown to support lymphocyte proliferation. We found the binding between stromal cells and lymphocytes to be highly selective and dependent on divalent cations; hence, specialized adhesion mechanisms may have a role in B cell development. Moreover, our studies suggest that phosphatidylinositol-anchored cell surface molecules may be involved in this adhesion. Our findings demonstrate the possibility that a single cell type provides physical support and proliferation stimuli for early B-lineage cells. This accessory cell is not a macrophage; rather, it has features of an endothelial or epithelial cell.

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“…The BP-1 antigen was identified as a homodimeric, phosphorylated cell surface glycoprotein on pre-B-cells and newly formed B cells in mouse bone marrow (1)(2)(3). This molecule, independently identified as a pre-B-cell neoplasiaassociated antigen and named 6C3 in another laboratory (4), is also expressed on certain bone marrow stromal cell lines and a subpopulation of thymic cortical epithelial cells (5)(6)(7).…”

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confidence: 99%

Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity.

Wang

Cooper

1993

Proc. Natl. Acad. Sci. U.S.A.

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The murine BP-1 antigen (also called 6C3) is a homodimeric, phosphorylated cell surface glycoprotein that is expressed on immature B-lineage cells, bone marrow stromal cell lines, thymic cortical epithelial cells, endothelial cells, enterocytes, and renal proximal tubular cells. The amino acid sequence deduced from a BP-1 cDNA predicted a type II integral membrane protein with a zinc-binding motif (His-GluXaa-Xaa-His) found in zinc-dependent metallopeptidases, and functional analysis suggested that BP-1 is aminopeptidase A [APA; L-a-aspartyl(L-ar-glutamyl)-peptide hydrolase, EC 3.4.11.71. Here we constructed an expression vector in which the BP-1 cDNA was placed downstream from the SRa promoter and used this construct to transfect COS-7 and Ltkcells. Both transfectants expressed the BP-1 antigen on the cell surface and APA activity. The enzymatic activity of recombinant APA was increased by Ca2+ and inhibited by Zn2+, consistent with previous reports with purified APA. Point mutation of one of the histidine residues in the zinc-binding motif to phenylalanine completely abolished APA enzymatic activity, suggesting the structure of the zinc-binding motif of APA is critical for catalytic activity. Both wild-type and mutant BP-1 were glycosylated, transported to the cell surface, and possessed molecular weights similar to native BP-1 molecules on the murine 18.81 pre-B-cell line. The successful expression of both wild-type and mutant APA should allow more precise analysis of the diverse physiological roles of this ectoenzyme.The differentiation of B cells requires interaction of lymphocyte precursors with other cell types in the microenvironments of fetal liver and adult bone marrow. Cell surface proteins expressed by developing B-lymphocyte precursors are thought to be involved in this highly complex, regulated process. The BP-1 antigen was identified as a homodimeric, phosphorylated cell surface glycoprotein on pre-B-cells and newly formed B cells in mouse bone marrow (1-3). This molecule, independently identified as a pre-B-cell neoplasiaassociated antigen and named 6C3 in another laboratory (4), is also expressed on certain bone marrow stromal cell lines and a subpopulation of thymic cortical epithelial cells (5-7).In mice, expression of the BP-1 (6C3) antigen on bone marrow-derived stromal cell lines correlates with their ability to support pre-B-cell growth (5). Interleukin 7 (IL-7) preferentially induces proliferation of bone marrow-derived pre-Bcells and up-regulates their expression of the BP-1 antigen (7,8), whereas its expression is extinguished in mature B lymphocytes and plasma cells (1). All of these observations suggest that the BP-1 molecule may play a crucial role in regulating growth and differentiation of early B-lineage cells.The BP-1 cDNA has been cloned recently (9), and the deduced amino acid sequence predicts a type II integral membrane protein with a zinc-binding motif-uncharged residue (Xaa)-Xaa-His-Glu-Xaa-Xaa-His-Xaa-hydrophobic residue-that is conserved among members of the zincd...

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Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products.

Cited by 49 publications

References 26 publications

Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc.

Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc.

Relationships between B‐lineage lymphocytes and stromal cells in long‐term bone marrow cultures

Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity.

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