Transformation and regeneration of garlic ( Allium sativum L.) by Agrobacterium -mediated gene transfer

Kondo, Tohru; Hasegawa, Hisakazu; Suzuki, Masahiko

doi:10.1007/s002990000222

Cited by 86 publications

(41 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Garlic is a sterile plant, which is propagated vegetatively, therefore genetic variation can be increased only by somaclonal variation, induced mutations or genetic transformation (Novak 1990;Burba 1993;Kondo et al 2000). Restoration of fertility and, therefore, of sexual reproduction would permit genetic studies and classical breeding of garlic.…”

Section: Introductionmentioning

confidence: 99%

Diversity in fertility potential and organo-sulphur compounds among garlics from Central Asia

et al. 2005

View full text Add to dashboard Cite

Abstract. Extending the collection of garlic (Allium sativum L.) accessions is an important means that is available for broadening the genetic variability of this cultivated plant, with regard to yield, quality, and tolerance to biotic and abiotic traits; it is also an important means for restoring fertility and flowering. In the framework of the EU project 'Garlic and Health', 120 garlic accessions were collected in Central Asia -the main centre of garlic diversity. Plants were documented and thereafter maintained in field collections in both Israel and The Netherlands. The collection was evaluated for biological and economic traits. Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development. A clear distinction was made between incomplete bolting and bolting populations; most of the accessions in the latter populations produced flowers with fertile pollen and receptive stigma. Wide variations were recorded with regard to differentiation of topsets, their size, number and rapidity of development. Furthermore, significant variation in organo-sulphur compounds (alliin, isoalliin, allicin and related dipeptides) was found within garlic collections and between plants grown under differing environmental conditions. Genetic fingerprinting by means of AFLP markers revealed three distinct groups within this collection, differing also in flowering ability and organo-S content.Abbreviations: AFLP TM -amplified fragment length polymorphism; HPLC-UV -high performance liquid chromatography ultra violet; PCA -principal component analysis;

show abstract

Section: Introductionmentioning

confidence: 99%

Diversity in fertility potential and organo-sulphur compounds among garlics from Central Asia

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The presence of a larger number of bacterial cells might enhance both the number of transformation events and tissue response related to biotic stress. Kondo et al (2000) reported that the periods of inoculation seem to be effective for the efficient transfer of the T-DNA into plant cells, and longer periods of inoculation gave negative effect on survival rate of explants. The inoculation time usually applied in transformation procedures is about 30 min for immature embryo of oil palm (Abdullah et al, 2005) and alfafa calli (Zhang et al, 2010), 40 min for embryogenic callus of P. tricuspidata (Yang et al, 2010), and up to 2 h for tobacco leaf ring (Vinod Kumar et al, 2005).…”

Section: Agrobacterium Densities and Inoculation Timesmentioning

confidence: 99%

The effect of Agrobacterium densities and inoculation times on gene transformation efficiency in rubber tree

Kalawong¹,

Srichuay²,

Te-chato³

2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Rubber tree belonging to the genus Hevea is an economically important crop of Thailand and Southeast Asia. To optimize its agronomical trait for glyphosate-resistant, in vitro gene transformation through Agrobacterium tumefaciens was investigated. The bacteria carrying plasmid pCAMBIA 1304, harboring gus as screenable marker genes and EPSPs gene was used. The shoot tips were immersed in A. tumefaciens suspension at optical densities (OD 600 ) at 0.3, 0.6 and 0.9 for various times (15, 30 and 60 min). The results revealed that shoot explants immersed in A. tumefaciens suspension at OD 600 of 0.6 for 30 min gave the higher survival rate after being cultured on glyphosate containing MS medium for one and half months. Assessment of transformed shoots revealed positive results in GUS histochemical assay. The presence of the gus and EPSPs genes in transformed rubber tree were confirmed by polymerase chain reaction (PCR) technique, dot blot hybridization and Southern PCR hybridization. Specific primers for the gus and EPSPs genes were designed to amplify a 919 and 1,600 bps DNA fragment, respectively.

show abstract

“…In the first report of the development of transgenic garlic, the optimization of the temperatures and duration of Agrobacterium co-cultivation with garlic calli derived from the apical meristem of cloves was carried out on the basis of reporter gene β-glucuronidase expression and the hygromycin phosphotransferase (HPT) selective gene (Kondo et al, 2000). As a subsequent alternative strategy, a particle bombardment-mediated system of garlic transformation was developed using apical meristem-derived calli (Park et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.)

Ahn

Yoon

Jeon

2013

Molecules and Cells

View full text Add to dashboard Cite

The genetic improvement of garlic plants (Allium sativum L.) with agronomical beneficial traits is rarely achieved due to the lack of an applicable transformation system. Here, we developed an efficient Agrobacterium-mediated transformation procedure with Danyang, an elite Korean garlic cultivar. Examination of sGFP (synthetic green fluorescence protein) expression revealed that treatment with 2-(N-morpholino) ethanesulfonic acid (MES), L-cysteine and/or dithiothreitol (DTT) gives the highest efficiency in transient gene transfer during Agrobacterium co-cultivation with calli derived from the roots of in vitro plantlets. To increase stable transformation efficiency, a two-step selection was employed on the basis of hygromycin resistance and sGFP expression. Of the hygromycin-resistant calli initially produced, only sGFP-expressing calli were subcultured for selection of transgenic calli. Transgenic plantlets produced from these calli were grown to maturity. The transformation efficiency increased up to 10.6% via our optimized procedure. DNA and RNA gel-blot analysis indicated that transgenic garlic plants stably integrated and expressed the phosphinothricin acetyltransferase (PAT) gene. A herbicide spraying assay demonstrated that transgenic plants of garlic conferred herbicide resistance, whilst nontransgenic plants and weeds died. These results indicate that our transformation system can be efficiently utilized to produce transgenic garlic plants with agronomic benefits.

show abstract

Transformation and regeneration of garlic ( Allium sativum L.) by Agrobacterium -mediated gene transfer

Cited by 86 publications

References 0 publications

Diversity in fertility potential and organo-sulphur compounds among garlics from Central Asia

Diversity in fertility potential and organo-sulphur compounds among garlics from Central Asia

The effect of Agrobacterium densities and inoculation times on gene transformation efficiency in rubber tree

Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.)

Contact Info

Product

Resources

About