Transformation and development of the flagellar apparatus ofCryptomonas ovata (Cryptophyceae) during cell division

Perasso, L.; Hill, David R.; Wetherbee, Richard

doi:10.1007/bf01384457

Cited by 20 publications

(17 citation statements)

References 22 publications

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“…atrorosea is similar to those of previous ultrastructural observations for cryptomonads (Roberts et al 1981, Gillott and Gibbs 1983, Roberts 1984, Hill and Wetherbee 1986, Perasso et al 1992) but is slightly more complex than those of other species. Specifically, R. reticulata var.…”

Section: Ultrastructure Of the Flagellar Apparatussupporting

confidence: 89%

“…Specifically, R. reticulata var. atrorosea had one more microtubular root than previously well-studied cryptomonads (Roberts et al 1981, Gillott and Gibbs 1983, Roberts 1984, Hill and Wetherbee 1986, Perasso et al 1992. R. reticulata var.…”

Section: Ultrastructure Of the Flagellar Apparatusmentioning

confidence: 71%

“…Φ in Gillott and Gibbs 1983) or the Cr root of Cryptomonas paramecium (Chilomonas paramecium in Roberts et al 1981), C. pyrenoidifera (C. ovata var. palustris in Roberts 1984) and C. ovata (Perasso et al 1992). The 3r is similar to the ascending microtubules of P. sulcata, H. phi, and G. theta; the twelvestranded microtubules of Cryptomonas paramecium; the four-stranded microtubules of C. pyrenoidifera; and the four-stranded microtubules of the diplomorphs of P. sulcata.…”

Section: Ultrastructure Of the Flagellar Apparatusmentioning

confidence: 77%

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Ultrastructure of the flagellar apparatus in Rhinomonas reticulata var. atrorosea (Cryptophyceae, Cryptophyta)

Nam¹,

Go²,

Son³

et al. 2013

ALGAE

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Rhinomonas reticulata var. atrorosea G. Novarino is a photosynthetic marine flagellate that is known to have typical characteristics of cryptomonads. We examined the flagellar apparatus of R. reticulata var. atrorosea by transmission electron microscopy. The major components of the flagellar apparatus of R. reticulata var. atrorosea consisted of four types of microtubular roots (1r, 2r, 3r, and mr), a non-keeled rhizostyle (Rhs), mitochondrion-associated lamella (ML), two connections between basal bodies, a striated fibrous root (SR) and a striated fiber-associated microtubular root (SRm). Four types of microtubular roots originated near the ventral basal body and extended toward the left side of the basal bodies. The non-keeled Rhs originated at the Rhs-associated striated fiber, which was located between two basal bodies and extended into the middle of the cell. The ML was a plate-like fibrous structure associated with mitochondria and originating from a Rhs-associated fiber. It split into two parts and extended toward the dorsal-posterior of the cell to a mitochondrion. The SR and SRm extended parallel to the anterior lobe of the cell. The overall configuration of the flagellar apparatus in R. reticulata var. atrorosea was similar to the previously reported descriptions of those of Cryptomonas paramecium, C. pyrenoidifera, C. ovata, Hanusia phi, Guillardia theta, and Proteomonas sulcata. However, the flagellar apparatus system of R. reticulata var. atrorosea was more complex than those of other cryptomonad species due to the presence of an additional microtubular root and other distinctive features, such as a rhizostyle-associated striated fiber and large ML.

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Section: Ultrastructure Of the Flagellar Apparatussupporting

confidence: 89%

Section: Ultrastructure Of the Flagellar Apparatusmentioning

confidence: 71%

Section: Ultrastructure Of the Flagellar Apparatusmentioning

confidence: 77%

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Ultrastructure of the flagellar apparatus in Rhinomonas reticulata var. atrorosea (Cryptophyceae, Cryptophyta)

Nam¹,

Go²,

Son³

et al. 2013

ALGAE

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“…(i) C ryptophyceae, C ryptomonas ovata , redrawn based on the data in Perasso et al . (). (j) G oniomonadea, G oniomonas avonlea , redrawn based on the data in Kim and Archibald ().…”

Section: Fundamental Features Of Mtocs: the Flagellar Apparatusmentioning

confidence: 97%

Evolution of microtubule organizing centers across the tree of eukaryotes

2013

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SUMMARYThe architecture of eukaryotic cells is underpinned by complex arrrays of microtubules that stem from an organizing center, referred to as the MTOC. With few exceptions, MTOCs consist of two basal bodies that anchor flagellar axonemes and different configurations of microtubular roots. Variations in the structure of this cytoskeletal system, also referred to as the 'flagellar apparatus', reflect phylogenetic relationships and provide compelling evidence for inferring the overall tree of eukaryotes. However, reconstructions and subsequent comparisons of the flagellar apparatus are challenging, because these studies require sophisticated microscopy, spatial reasoning and detailed terminology. In an attempt to understand the unifying features of MTOCs and broad patterns of cytoskeletal homology across the tree of eukaryotes, we present a comprehensive overview of the eukaryotic flagellar apparatus within a modern molecular phylogenetic context. Specifically, we used the known cytoskeletal diversity within major groups of eukaryotes to infer the unifying features (ancestral states) for the flagellar apparatus in the Plantae, Opisthokonta, Amoebozoa, Stramenopiles, Alveolata, Rhizaria, Excavata, Cryptophyta, Haptophyta, Apusozoa, Breviata and Collodictyonidae. We then mapped these data onto the tree of eukaryotes in order to trace broad patterns of trait changes during the evolutionary history of the flagellar apparatus. This synthesis suggests that: (i) the most recent ancestor of all eukaryotes already had a complex flagellar apparatus, (ii) homologous traits associated with the flagellar apparatus have a punctate distribution across the tree of eukaryotes, and (iii) streamlining (trait losses) of the ancestral flagellar apparatus occurred several times independently in eukaryotes.

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“…It has been shown that cell asymmetry and polarity in cryptomonads is transferred to daughter cells via a complex cell division process, reorganization of the flagellar apparatus, and reorientation of organelles at division (Perasso et al 1992(Perasso et al , 1993. This study on the cell surface of K. caudata has shown that the growth of the cell surface is also spatially and temporally controlled.…”

Section: Development Of the Cell Surface And The Maintenance Of Cell mentioning

confidence: 98%

The surface periplast component of the protistKomma caudata (Cryptophyceae) self-assembles from a secreted high-molecular-mass polypeptide

1997

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Summary. The cell covering of the cryptomonad Komma caudata(Geitler) Hill is a trilaminar structure consisting of a surface periplast component (SPC) and an inner periplast component (IPC) that sandwich the plasma membrane. In order to investigate the development of the periplast, we have raised monoclonal antibodies against the cell surface of K. caudata. Immunoblot analyses using one of these antibodies, K1/D.10, showed that it labeled a high-molecular-mass polypeptide. Immunofluorescence and pre-and post-embedding immunogold labeling studies demonstrated that the antibody recognized sites on the cell surface corresponding to the SPC plates and another K. caudata cell surface component, the rosulate scales. Labeling was also detected on surface domains devoid of periplast, namely the vestibular/gullet region of the cell. Post-embedding immunocytochemistry revealed that intracellular sites labeled with K1/D.10 included the Golgi apparatus and its associated vesicles. We propose that the subunits of the K. caudata cell covering are antigenically related molecules and that they self-assemble on the cell surface after secretion via the endomembrane system and deployment at the vestibular/gullet region or, in dividing cells, the cytokinetic furrow.

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Transformation and development of the flagellar apparatus ofCryptomonas ovata (Cryptophyceae) during cell division

Cited by 20 publications

References 22 publications

Ultrastructure of the flagellar apparatus in Rhinomonas reticulata var. atrorosea (Cryptophyceae, Cryptophyta)

Ultrastructure of the flagellar apparatus in Rhinomonas reticulata var. atrorosea (Cryptophyceae, Cryptophyta)

Evolution of microtubule organizing centers across the tree of eukaryotes

The surface periplast component of the protistKomma caudata (Cryptophyceae) self-assembles from a secreted high-molecular-mass polypeptide

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