Transformation and characterization of transgenic Bidens pilosa L.

Wang, Chen-Kuen; Hsu, Shin-Yun; Chen, Po‐Yen; To, Kin‐Ying

doi:10.1007/s11240-011-0110-9

Cited by 14 publications

(14 citation statements)

References 21 publications

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“…Agrobacterium-mediated transformation was carried out as previously described [17] with modifications. For pre-culture prior to Agrobacterium infection, cotyledons or leaves were excised from 2-to 3-week-old in vitro plantlets of P. hybrida one day before infection, and incubated in KCMS solid medium supplemented with 100 µM acetosyringone for one day.…”

Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%

Transforming the Snapdragon Aurone Biosynthetic Genes into Petunia Alters Coloration Patterns in Transgenic Flowers

Wang¹,

Chin²,

Lin³

et al. 2015

ABB

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Aurones belong to a small class of flavonoids that provide yellow color in some floricultural plants including snapdragon. To explore novel flower coloration, two full-length cDNAs encoding chalcone 4'-O-glucosyltransferase (designated as SRY4'CGT) and aureusidin synthase (designated as SRYAS1) in the aurone biosynthetic pathway were cloned from yellow flowers of snapdragon (Antirrhinum majus cv. Ribbon Yellow). Binary vectors were constructed and transformed separately into Petunia hybrida harboring blue flowers. Only a few flowers in 4 out of 9 transgenic SRY4'CGT plants showed variegated blue-white sectors; as time passed, amounts of variegated flowers and proportion of white sectors in the background blue color of the new-born flowers gradually increased, until finally, the petal color was completely white in all late-born flowers. In contrast, a few flowers in 3 out of 13 transgenic SRYAS1 plants showed variegated blue-white sectors; but, the amounts of variegated flowers did not increase over the whole flowering stage, and no complete white flowers were observed. RNA samples isolated from blue and white sectors of T1 transgenic SRY4'CGT plants were analyzed by reverse transcription-PCR, transgenic SRY4'CGT transcripts were detected in both sectors; however, transcripts of an upstream gene, chalcone synthase (CHS), were abundantly detected in the blue sectors but largely reduced in the white sectors, suggesting that the expression of CHS gene was suppressed in white sectors of transgenic plants. Furthermore, HPLC coupled with mass spectrometry demonstrated cyandin, malvidin and their derivatives were absent in white sectors, causing the white phenotype. Our findings may be attractive to molecular breeders.

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Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%

Transforming the Snapdragon Aurone Biosynthetic Genes into Petunia Alters Coloration Patterns in Transgenic Flowers

Wang¹,

Chin²,

Lin³

et al. 2015

ABB

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“…11) is considered to be a false positive line, since the hptII selection marker was detected in its genome and thus was able to grow in 50 mg l −1 hygromycin-containing media R2S and NBS during selection; however, the foreign maize genomic pepc DNA fragment was not detected, probably due to incomplete T-DNA integration in Agrobacterium infection. Loss of one of the two transgenes within the same T-DNA is a frequent event, and has been reported in a range of plants including wheat (Wu et al 2006), rice (Kim et al 2003), and our recent studies in Bidens pilosa (Wang et al 2012) and Cleome spinosa (Tsai et al 2012). Similarly, to verify transgenic C. spinosa plants, genomic DNA was isolated and PCR analysis was carried out using specific primers for pepc (Fig.…”

Section: Resultsmentioning

confidence: 95%

Transformation and characterization of transgenic rice and Cleome spinosa plants carrying the maize phosphoenolpyruvate carboxylase genomic DNA

Chen

Tsai

et al. 2016

Plant Cell Tiss Organ Cult

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“…Previously, we established optimal conditions for tissue culture, plant regeneration, and Agrobacterium-mediated transformation in B. pilosa var. radiata [30]. In this study, expression vectors (Supplementary Figure S1) containing two full-length cDNAs that encode ∆12-oleate desaturase (pBPOD) and ∆12-fatty acid acetylenase (pBPFAA) were transformed independently into B. pilosa var.…”

Section: Discussionmentioning

confidence: 99%

Transformation and Characterization of Δ12-Fatty Acid Acetylenase and Δ12-Oleate Desaturase Potentially Involved in the Polyacetylene Biosynthetic Pathway from Bidens pilosa

Chen

Hsieh

Tsai

et al. 2020

Plants

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Bidens pilosa is commonly used as an herbal tea component or traditional medicine for treating several diseases, including diabetes. Polyacetylenes have two or more carbon–carbon triple bonds or alkynyl functional groups and are mainly derived from fatty acid and polyketide precursors. Here, we report the cloning of full-length cDNAs that encode 12-fatty acid acetylenase (designated BPFAA) and 12-oleate desaturase (designated BPOD) from B. pilosa, which we predicted to play a role in the polyacetylene biosynthetic pathway. Subsequently, expression vectors carrying BPFAA or BPOD were constructed and transformed into B. pilosa via the Agrobacterium-mediated method. Genomic PCR analysis confirmed the presence of transgenes and selection marker genes in the obtained transgenic lines. The copy numbers of transgenes in transgenic lines were determined by Southern blot analysis. Furthermore, 4–5 FAA genes and 2–3 OD genes were detected in wild-type (WT) plants. Quantitative real time-PCR revealed that some transgenic lines had higher expression levels than WT. Western blot analysis revealed OD protein expression in the selected transformants. High-performance liquid chromatography profiling was used to analyze the seven index polyacetylenic compounds, and fluctuation patterns were found.

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Transformation and characterization of transgenic Bidens pilosa L.

Cited by 14 publications

References 21 publications

Transforming the Snapdragon Aurone Biosynthetic Genes into Petunia Alters Coloration Patterns in Transgenic Flowers

Transforming the Snapdragon Aurone Biosynthetic Genes into Petunia Alters Coloration Patterns in Transgenic Flowers

Transformation and characterization of transgenic rice and Cleome spinosa plants carrying the maize phosphoenolpyruvate carboxylase genomic DNA

Transformation and Characterization of Δ12-Fatty Acid Acetylenase and Δ12-Oleate Desaturase Potentially Involved in the Polyacetylene Biosynthetic Pathway from Bidens pilosa

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