Transformação genética de laranja doce (Citrus sinensis L. Osbeck) com o gene D4E1 dirigido pelos promotores CaMV35S ou AtPP2

Abstract

A Deus, pela presença constante em minha vida, por me dar força e perseverança. Obrigada pelos momentos de felicidade, pela oportunidade de realizar este trabalho, pelas dificuldades e experiências ao longo deste doutorado, que também fazem parte do meu aprendizado.Ao professor Dr. Francisco de Assis Alves Mourão Filho, primeiramente pela oportunidade e confiança, também pela paciência, orientação e ensinamentos durante este período.

Transgenic Sweet Orange Expressing the Sarcotoxin IA Gene Produces High-Quality Fruit and Shows Tolerance to ‘Candidatus Liberibacter asiaticus’

“…However, variations in the levels of the stx IA gene expression did not indicate a relationship with the number of transgene copy insertions, as all STX lines included in this study have just one copy of the stx IA gene in their genome [ 37 ]. Similar results were also found for other transgenic citrus plants involving different gene constructions [ 35 , 50 ]. Variations in transgenes expression in plants containing the same gene construction may be related to the position where the gene was inserted in the genome [ 50 , 51 ].…”

“…Similar results were also found for other transgenic citrus plants involving different gene constructions [ 35 , 50 ]. Variations in transgenes expression in plants containing the same gene construction may be related to the position where the gene was inserted in the genome [ 50 , 51 ].…”

“…On the other hand, plants of the other two transgenic lines showed intermediate levels of the stx IA gene expression ( Figure 3 ). Differences in the expression of genes encoding antimicrobial peptides had been previously reported [ 50 ], but not for citrus plants expressing the stx IA gene. However, variations in the levels of the stx IA gene expression did not indicate a relationship with the number of transgene copy insertions, as all STX lines included in this study have just one copy of the stx IA gene in their genome [ 37 ].…”

“…However, the control and the STX-13 plants showed the highest HLB incidence ( Figure 4 ), while the STX-5 plants, which had the highest stx IA gene expression ( Figure 3 ), exhibited the lowest HLB incidence, similarly to the STX-11 line ( Figure 4 ). Unlike other citrus diseases, such as citrus canker, which only causes localized infections/lesions and has been the target of many studies with transgenic plants expressing genes that encode AMPs [ 37 , 49 , 50 ], HLB is a systemic insect-vectored bacterial disease showing complex systemic movement within the plant [ 52 ]. Further, the C Las bacterium is restricted to the phloem vessels of the citrus plants moving and redistributing all over the citrus plant [ 53 ].…”