Transferring the exudate in the tissue engineering chamber as a trigger to incubate large amount adipose tissue in remote area

Chen, Lei; Dong, Ziqing; Wan, Jinlin; Xiao, Xiaolian; Lu, Feng; Wang, Biao

doi:10.1002/term.2580

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…Current reconstructive strategies include the use of autologous flaps, fat grafting, and allogeneic/synthetic fillers. 1,2 These methods, however, have limitations, including donor-site morbidity, unpredictable absorption rate, and rejection reactions, respectively. [3][4][5] Induction of in situ tissue regeneration may overcome these limitations.…”

Section: Introductionmentioning

confidence: 99%

External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin

Zhang

Cai

et al. 2020

Tissue Engineering Part A

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External volume expansion (EVE) is an effective method of adipose tissue regeneration. However, it remains unclear how EVE induces adipose tissue regeneration. In this study, we developed EVE devices to generate expanded prefabricated adipose tissue (EPAT) in rats and investigated cell proliferation, adipogenesis, and the expression of extracellular matrix (ECM) proteins during the 12 weeks suction. In addition, EPAT-generated decellularized adipose tissue (DAT) was used to assess the role of ECM proteins in cell proliferation and differentiation. Matrix deposition was significantly increased after EVE suction, with fibronectin and laminin showing the most dramatic changes. Fibronectin expression peaked during weeks 1-4, when Ki67 cells in EPAT peaked. Laminin expression peaked during weeks 8-12, when peroxisome proliferator-activated receptor-g expression also peaked. In vitro, adipose-derived stem cells (ASCs) displayed a higher proliferation rate in week 1 DAT, when fibronectin expression was highest, whereas ASC adipogenesis was significantly higher on week 12 DAT, when laminin expression was abundant. These results showed that EVE device enhanced ECM deposition, which is closely related to cell proliferation and differentiation.

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Section: Introductionmentioning

confidence: 99%

External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin

Zhang

Cai

et al. 2020

Tissue Engineering Part A

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“…This is most likely due to macrophage production either through direct secretion or through indirect proteolytic extra-cellular matrix remodeling of pro-angiogenic factors such as vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF) or tumor necrosis factor-α (TNF-α) as well as the pro-adipogenic factor, fibroblast growth factor-2 (FGF-2) 23 . This paracrine communication was clearly demonstrated in another in vivo model (fluid drainage model), where the exudate found within the TEC also had the ability to trigger adipogenesis remotely 14 . In a TEC model for bone formation, an alternative to a perforated dome was the presence of mesh walls, which showed improved angiogenesis and tissue growth within the porous TEC 24,25 .…”

Section: Discussionmentioning

confidence: 75%

“…As a consequence of these complementary effects, the TEC acts as a bona fide bioreactor promoting an in vivo fivefold increase of the fat flap volume within several months 10 . This TEC technology has been experimentally used in a wide range of animal models including mice 11 , rats 4,12,13 , rabbits 14 and pigs 10 . More recently, Morrison and al demonstrated the surgical feasibility and safety of the TEC for breast reconstruction in a first-in-human trial 15 .…”

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confidence: 99%

Rationale for the design of 3D-printable bioresorbable tissue-engineering chambers to promote the growth of adipose tissue

Faglin

Gradwohl

Depoortère

et al. 2020

Sci Rep

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Tissue engineering chambers (TECs) bring great hope in regenerative medicine as they allow the growth of adipose tissue for soft tissue reconstruction. To date, a wide range of TEC prototypes are available with different conceptions and volumes. Here, we addressed the influence of TEC design on fat flap growth in vivo as well as the possibility of using bioresorbable polymers for optimum TEC conception. In rats, adipose tissue growth is quicker under perforated TEC printed in polylactic acid than non-perforated ones (growth difference 3 to 5 times greater within 90 days). Histological analysis reveals the presence of viable adipocytes under a moderate (less than 15% of the flap volume) fibrous capsule infiltrated with CD68 + inflammatory cells. CD31-positive vascular cells are more abundant at the peripheral zone than in the central part of the fat flap. Cells in the TEC exhibit a specific metabolic profile of functional adipocytes identified by 1 H-NMR. Regardless of the percentage of TEC porosity, the presence of a flat base allowed the growth of a larger fat volume (p < 0.05) as evidenced by MRI images. In pigs, bioresorbable TEC in poly[1,4-dioxane-2,5-dione] (polyglycolic acid) PURASORB PGS allows fat flap growth up to 75 000 mm 3 at day 90, (corresponding to more than a 140% volume increase) while at the same time the TEC is largely resorbed. No systemic inflammatory response was observed. Histologically, the expansion of adipose tissue resulted mainly from an increase in the number of adipocytes rather than cell hypertrophy. Adipose tissue is surrounded by perfused blood vessels and encased in a thin fibrous connective tissue containing patches of CD163 + inflammatory cells. Our large preclinical evaluation defined the appropriate design for 3D-printable bioresorbable TECs and thus opens perspectives for further clinical applications. Reconstruction of adipose tissue defects after surgery (e.g. mastectomy) or trauma remains a major surgical challenge. Numerous tissue-engineering techniques were attempted experimentally. It includes repair methods such as autologous or heterologous fat grafting or also transfer of vascularized adipose tissue from a donor site, also called a fat flap. The limitation of this latter method is that the fat flap requires a sufficient amount of available fat in the donor site, which often leads to deformity at the donor site. Moreover, donor flap sites may not

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“…In our study, we used a TEC technique that introduced an intrinsic vessel into the constructs. 41 Although cartilage is an avascular tissue that contains chondrocytes alone, the exchange of oxygen and nutrients is required during regeneration. 42 In fact, the CARTILAGE TISSSUE ENGINEEERING WITHOUT CELL CULTURE perichondrium is a well-vascularized tissue.…”

Section: Discussionmentioning

confidence: 99%

Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture

Wang

Chen

et al. 2020

Tissue Engineering Part C: Methods

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Background: Clinically available cartilage, such as large-volume tissue-engineered cartilage, is urgently required for various clinical applications. Tissue engineering chamber (TEC) models are a promising organlevel strategy for efficient enlargement of cells or tissues within the chamber. The conventional TEC technology is not suitable for cartilage culture, because it lacks the necessary chondrogenic growth factor, which is present in platelet-rich plasma (PRP). In this study, we added autogenous auricular cartilage fragments mixed with PRP in a TEC to obtain a large amount of engineered cartilage. Experiment: To prove the efficacy of this method, 48 New Zealand white rabbits were randomly divided into 4 groups: PRP, vascularized (Ves), PRP, PRP+Ves, and control. Auricular cartilage was harvested from the rabbits, cut into fragments (2 mm), and then injected into TECs. Cartilage constructs were harvested at week 8, and construct volumes were measured. Histological morphology, immunochemical staining, and mechanical strength were evaluated. Results: At week 8, PRP+Ves constructs developed a white, cartilage-like appearance. The volume of cartilage increased by 600% the original volume from 0.30 to 1.8-0.1789 mL. Histological staining showed proliferation of edge chondrocytes in the embedded cartilage in the PRP and PRP+Ves groups. Furthermore, the cartilage constructs in the PRP+Ves group show mechanical characteristics similar to those of normal cartilage. Conclusions: Auricular cartilage fragments mixed with PRP and vascularization of the TEC showed a significantly increased cartilage tissue volume after 8 weeks of incubation in rabbits.

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Transferring the exudate in the tissue engineering chamber as a trigger to incubate large amount adipose tissue in remote area

Cited by 7 publications

References 39 publications

External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin

External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin

Rationale for the design of 3D-printable bioresorbable tissue-engineering chambers to promote the growth of adipose tissue

Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture

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