Transferrin Decorated Thermoresponsive Nanogels as Magnetic Trap Devices for Circulating Tumor Cells

Asadian‐Birjand, Mazdak; Biglione, Catalina; Bergueiro, Julián; Cappelletti, Ariel Leonardo; Rahane, Chinmay Satish; Chate, Govind P.; Khandare, Jayant; Klemke, Bastian; Strumia, Miriam Cristina; Calderón, Marcelo

doi:10.1002/marc.201500590

Cited by 26 publications

(27 citation statements)

References 33 publications

(44 reference statements)

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“…FT-IR spectroscopy demonstrated the presence of magnetite due to the band at 590 cm −1 corresponding to the Fe-O tension of the magnetite ( Figure S1). In a second stage, MNPs were modified with APTES using an ultrasound-assisted method previously reported by our group [36]. We found that the size of the nanoparticles was not altered by the modification with the silane agent, maintaining an average size of 5 ± 2 nm ( Figure S2a).…”

Section: Mnp@bcn Synthesismentioning

confidence: 54%

“…MNPs were modified following a previously reported methodology [36]. Briefly, 50 mg of MNPs were dissolved in 26 mL of a mixture EtOH:water (1:1).…”

Section: Aptes Modification Of Mnp With An Ultrasonic Horn Approach (mentioning

confidence: 99%

“…For the synthesis of MNGs, we used a methodology developed in a previous work that consisted in a novel ultrasound-assisted technique based on thermonanoprecipitation [36]. This surfactant-free method allowed the synthesis of MNGs with controlled size and in short reaction times such as 15 min.…”

Section: Mng@tf Synthesismentioning

confidence: 99%

“…In a previous work, we demonstrated a powerful methodology for the synthesis of MNGs on the basis of an ultrasound-assisted, alkyne-azide strain-promoted cycloaddition as a crosslinking reaction [36]. Magnetic nanoparticles functionalized with bicyclononyne (MNP@BCN) and azidated thermoresponsive linear polyglycerol (tPG) were used as building blocks for synthesizing thermoresponsive MNGs.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

et al. 2018

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Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system.

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Section: Mnp@bcn Synthesismentioning

confidence: 54%

“…MNPs were modified following a previously reported methodology [36]. Briefly, 50 mg of MNPs were dissolved in 26 mL of a mixture EtOH:water (1:1).…”

Section: Aptes Modification Of Mnp With An Ultrasonic Horn Approach (mentioning

confidence: 99%

Section: Mng@tf Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

et al. 2018

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“…The authors modified magnetic particles with BCN serving for both crosslinking and conjugation purposes. The modification of the reactive precursor with a thermoresponsive polymer and transferrin resulted in a magnetic nanogel, decorated with the desired protein …”

Section: Click Chemistry For Functionalization Of Reactive Particlesmentioning

confidence: 99%

Reactive Precursor Particles as Synthetic Platform for the Generation of Functional Nanoparticles, Nanogels, and Microgels

Gruber

Navarro

Klinger

2019

Adv Materials Inter

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Precise control of the chemical functionality of polymer nanoparticles is a key requirement in tailoring their (dynamic) colloidal properties toward advanced applications. However, current synthetic techniques are still limited in the versatility of chemical design and preparation of such functional colloidal nanomaterials. Two major challenges remain: First, various particle preparation methods are restricted in their functional group tolerance, thus hindering certain combinations of polymer backbones with specific functional groups. Second, the preparation of particles with different functionalities requires the synthesis of different particle batches. But this often results in a simultaneous variation of colloidal features. As a result, the accurate determination of important structure–property relations is still hindered. To address these restrictions, postmodification of preformed reactive particles is gaining more attention. This technique has evolved from polymer synthesis, where postpolymerization functionalization enables the introduction of a plethora of functional groups without changing the degree of polymerization and the molecular weight distribution. Similarly, modifying precursor particles enables the introduction of functional groups into particles while reducing variations in colloidal features, e.g., particle size and size distribution. This powerful synthetic method complements established procedures for functionalization of particle surfaces, thereby enabling the facile preparation of (multi‐)functional particle libraries, which will allow precise investigations of structure–property relations.

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Selective Cell Isolation by Transferrin Functionalized Silane–Carbon Soot Mediated Superhydrophobic Micropatterns

Chate¹,

Kale²,

Khobragade³

et al. 2018

Adv Materials Inter

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area was performed using orange II dye method. This amine group quantitation method was used to confirm silanization. Consequently, silane-functionalized glass slips were then subjected to Tf conjugation at marked sites where patterns were developed. Tf was chemically immobilized with aminefunctionalized silanated glass using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide HCl (EDC · HCl) coupling reaction. The silanization provides the necessary amine groups to be

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Transferrin Decorated Thermoresponsive Nanogels as Magnetic Trap Devices for Circulating Tumor Cells

Cited by 26 publications

References 33 publications

Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

Reactive Precursor Particles as Synthetic Platform for the Generation of Functional Nanoparticles, Nanogels, and Microgels

Selective Cell Isolation by Transferrin Functionalized Silane–Carbon Soot Mediated Superhydrophobic Micropatterns

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