Transferrin conjugation does not increase the efficiency of liposomal Foscan during in vitro photodynamic therapy of oesophageal cancer

Paszko, Edyta; Vaz, Gisela M. F.; Ehrhardt, Carsten; Senge, Ma­thias O.

doi:10.1016/j.ejps.2012.10.018

Cited by 24 publications

(21 citation statements)

References 43 publications

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“…32 and dipalmitoyl phosphatidylglycerol (DPPG) was 31%. 33 In addition, the cross-linking efficiency of Tf on liposomes composed of DSPE-PEG(2000), DSPE-PEG(3400), distearoylphosphatidylcholine (DSPC), and cholesterol was 13.9%, which corresponded to 112 Tf molecules in a liposome. 34 When 1% (w/mol) Tf was used, 313 Tf molecules has been observed in a liposome composed of DOPE, DSPE-PEG(2000), and cholesteroyl hemisuccinate.…”

Section: Resultsmentioning

confidence: 99%

Neuroprotection Against Degeneration of SK-N-MC Cells Using Neuron Growth Factor-Encapsulated Liposomes with Surface Cereport and Transferrin

Kuo

Chou

2014

Journal of Pharmaceutical Sciences

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Section: Resultsmentioning

confidence: 99%

Neuroprotection Against Degeneration of SK-N-MC Cells Using Neuron Growth Factor-Encapsulated Liposomes with Surface Cereport and Transferrin

Kuo

Chou

2014

Journal of Pharmaceutical Sciences

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“…The human esophageal squamous cell carcinoma cell line OE21 [17] and the human adenocarcinoma cell lines SKGT-4 [18] and OE33 [19], derived from Barrett’s esophagus, were purchased from the European Collection of Cell Cultures (ECACC).…”

Section: Methodsmentioning

confidence: 99%

“…Even, natural light can activate these photoactive compounds leading to their photodegradation and also they may prematurely produce cellular damage. Therefore most experimental manipulations with the living cellular materials have to be carried out under special illumination conditions [7], [16], [17]. 16 By their very nature these compounds have an intrinsic fluorescence over a broad range of excitation and emission wavelengths hence making difficult to use conventional assays that are broadly used for a drug evaluation.…”

Section: Introductionmentioning

confidence: 99%

High Content Screening as High Quality Assay for Biological Evaluation of Photosensitizers In Vitro

et al. 2013

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A novel single step assay approach to screen a library of photdynamic therapy (PDT) compounds was developed. Utilizing high content analysis (HCA) technologies several robust cellular parameters were identified, which can be used to determine the phototoxic effects of porphyrin compounds which have been developed as potential anticancer agents directed against esophageal carcinoma. To demonstrate the proof of principle of this approach a small detailed study on five porphyrin based compounds was performed utilizing two relevant esophageal cancer cell lines (OE21 and SKGT-4). The measurable outputs from these early studies were then evaluated by performing a pilot screen using a set of 22 compounds. These data were evaluated and validated by performing comparative studies using a traditional colorimetric assay (MTT). The studies demonstrated that the HCS assay offers significant advantages over and above the currently used methods (directly related to the intracellular presence of the compounds by analysis of their integrated intensity and area within the cells). A high correlation was found between the high content screening (HCS) and MTT data. However, the HCS approach provides additional information that allows a better understanding of the behavior of these compounds when interacting at the cellular level. This is the first step towards an automated high-throughput screening of photosensitizer drug candidates and the beginnings of an integrated and comprehensive quantitative structure action relationship (QSAR) study for photosensitizer libraries.

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“…Dendrimer phthalocyanine encapsulated polymeric micelle showed higher PDT efficacy in mice bearing human lung adenocarcinoma A549 cells, enhanced photocytotoxicity upon photoirradiation and accumulated in endolysosomes than Photofrin-2 (Nishiyama et al, 2009). However, polyethylene-glycol(PEG)-grafted transferrin-conjugated liposomes of second-generation photosensitizer 5, 10, 15, 20-tetra ( m -hydroxyphenyl) chlorin (Foscan) did not improved the photocytotoxicity or intracellular accumulation of Foscan in esophageal cancer cell line when compared to unmodified liposomes (Paszko et al, 2013). Chitosan functionalized pluronic nanogel containing gold nanorod as photothermal therapy agent and chlorine e6 (Ce6) as photosensitizer for PDT enhanced tumor ablation in-vivo by combination of PDT followed by photothermal therapy compared to single therapy (Kim et al, 2013).…”

Section: Physical Approaches To Overcome Mdrmentioning

confidence: 99%

Nanodrug delivery in reversing multidrug resistance in cancer cells

et al. 2014

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Different mechanisms in cancer cells become resistant to one or more chemotherapeutics is known as multidrug resistance (MDR) which hinders chemotherapy efficacy. Potential factors for MDR includes enhanced drug detoxification, decreased drug uptake, increased intracellular nucleophiles levels, enhanced repair of drug induced DNA damage, overexpression of drug transporter such as P-glycoprotein(P-gp), multidrug resistance-associated proteins (MRP1, MRP2), and breast cancer resistance protein (BCRP). Currently nanoassemblies such as polymeric/solid lipid/inorganic/metal nanoparticles, quantum dots, dendrimers, liposomes, micelles has emerged as an innovative, effective, and promising platforms for treatment of drug resistant cancer cells. Nanocarriers have potential to improve drug therapeutic index, ability for multifunctionality, divert ABC-transporter mediated drug efflux mechanism and selective targeting to tumor cells, cancer stem cells, tumor initiating cells, or cancer microenvironment. Selective nanocarrier targeting to tumor overcomes dose-limiting side effects, lack of selectivity, tissue toxicity, limited drug access to tumor tissues, high drug doses, and emergence of multiple drug resistance with conventional or combination chemotherapy. Current review highlights various nanodrug delivery systems to overcome mechanism of MDR by neutralizing, evading, or exploiting the drug efflux pumps and those independent of drug efflux pump mechanism by silencing Bcl-2 and HIF1α gene expressions by siRNA and miRNA, modulating ceramide levels and targeting NF-κB. “Theragnostics” combining a cytotoxic agent, targeting moiety, chemosensitizing agent, and diagnostic imaging aid are highlighted as effective and innovative systems for tumor localization and overcoming MDR. Physical approaches such as combination of drug with thermal/ultrasound/photodynamic therapies to overcome MDR are focused. The review focuses on newer drug delivery systems developed to overcome MDR in cancer cell.

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Transferrin conjugation does not increase the efficiency of liposomal Foscan during in vitro photodynamic therapy of oesophageal cancer

Cited by 24 publications

References 43 publications

Neuroprotection Against Degeneration of SK-N-MC Cells Using Neuron Growth Factor-Encapsulated Liposomes with Surface Cereport and Transferrin

Neuroprotection Against Degeneration of SK-N-MC Cells Using Neuron Growth Factor-Encapsulated Liposomes with Surface Cereport and Transferrin

High Content Screening as High Quality Assay for Biological Evaluation of Photosensitizers In Vitro

Nanodrug delivery in reversing multidrug resistance in cancer cells

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