Transferrin Adsorption onto PLGA Nanoparticles Governs Their Interaction with Biological Systems from Blood Circulation to Brain Cancer Cells

Chang, Jiang; Paillard, Archibald; Passirani, Catherine; Morille, Marie; Benoit, Jean‐Pierre; Betbeder, Didier; Garcion, Emmanuel

doi:10.1007/s11095-011-0624-1

Cited by 96 publications

(66 citation statements)

References 65 publications

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“…CD71 expression is particularly increased in metastatic and drug resistant tumors [33][34][35][36][37][38]. This makes CD71 both a tumor marker and a possible system for the delivery of small-molecule drugs to these cells by CD71-mediated endocytosis [39][40][41][42][43][44]. Recently, CD71 was identified as the receptor for HFt [22], with distinct binding sites for HFt and transferrin [45].…”

Section: Discussionmentioning

confidence: 99%

Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents

et al. 2017

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Abstract:The ability of ferritin to bind and deliver metals and metal-based drugs to human neuroblastoma SH-SY5Y cells was studied. We used heavy chain (H) ferritin-based metal-containing nanocarriers to test whether these constructs, which are able to cross the blood-brain barrier, may be used for the delivery of toxic molecules to brain cells, and to study their effect on the viability and cellular redox homeostasis of human neuroblastoma cells. We show that metal-containing nanocarriers are efficiently captured by SH-SY5Y cells. Iron-containing nanocarriers have a proliferative effect, while silver and cisplatin-encapsulated nanocarriers determine concentration-dependent neuroblastoma cell death. This work is a proof of concept for the use of ferritins for the delivery of toxic molecules to brain tumors.

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Section: Discussionmentioning

confidence: 99%

Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents

et al. 2017

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“…The composite nanoparticles were collected by centrifugation at 30,000× g for 30 minutes (3K30; Sigma Centrifuge, UK) and washed twice with distilled water before re-suspending in 5% w/v aqueous trehalose solution and freeze-dried (Advantage, VirTis, Gardiner, NY, USA) using a protocol modified from Jain et al 29 To investigate the effect of surface modification on transfection efficiency, Tf-appended composite nanoparticles were also prepared. Transferrin was adsorbed on the surface of nanoparticles using modifications of reported methods 30,31 suspending the nanoparticles in 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer, pH 7.4, with 150 mM NaCl containing transferrin. The resultant nanoparticles were collected by centrifugation at 25,000× g for 30 minutes.…”

Section: Formulation Of Composite Nanoparticles By Double Emulsion Somentioning

confidence: 99%

Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Jain¹,

Massey²,

Yusuf³

et al. 2015

IJN

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We report the formulation of novel composite nanoparticles that combine the high transfection efficiency of cationic peptide-DNA nanoparticles with the biocompatibility and prolonged delivery of polylactic acid–polyethylene glycol (PLA-PEG). The cationic cell-penetrating peptide RALA was used to condense DNA into nanoparticles that were encapsulated within a range of PLA-PEG copolymers. The composite nanoparticles produced exhibited excellent physicochemical properties including size <200 nm and encapsulation efficiency >80%. Images of the composite nanoparticles obtained with a new transmission electron microscopy staining method revealed the peptide-DNA nanoparticles within the PLA-PEG matrix. Varying the copolymers modulated the DNA release rate >6 weeks in vitro. The best formulation was selected and was able to transfect cells while maintaining viability. The effect of transferrin-appended composite nanoparticles was also studied. Thus, we have demonstrated the manufacture of composite nanoparticles for the controlled delivery of DNA.

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“…Titration of phospholipids in samples was performed on filtrates by Bartlett method, 14,15 BM-loaded poly(lactic-co-glycolic) acid nanoparticles (Plga BM nanoparticles) Blank nanoparticles were obtained by a modified solvent diffusion (nanoprecipitation) technique. 16 Briefly, PLGA (10 mg) was solubilized in acetone (850 µL), and then 150 µL ethanol was added. This organic phase was quickly poured into 10 mL deionized water (aqueous phase) and kept stirring at 1,000 rpm for 3 hours.…”

Section: Bm-loaded Dspe-peg Micelles (Dspe-peg Bm Micelles)mentioning

confidence: 99%

Nano-sized cytochrome P450 3A4 inhibitors to block hepatic metabolism of docetaxel

Paolini

Poul

Berjaud

et al. 2017

IJN

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Most drugs are metabolized by hepatic cytochrome P450 3A4 (CYP3A4), resulting in their reduced bioavailability. In this study, we present the design and evaluation of bio-compatible nanocarriers trapping a natural CYP3A4-inhibiting compound. Our aim in using nanocarriers was to target the natural CYP3A4-inhibiting agent to hepatic CYP3A4 and leave drug-metabolizing enzymes in other organs undisturbed. In the design of such nanocarriers, we took advantage of the nonspecific accumulation of small nanoparticles in the liver. Specific targeting functionalization was added to direct nanocarriers toward hepatocytes. Nanocarriers were evaluated in vitro for their CYP3A4 inhibition capacity and in vivo for their biodistribution, and finally injected 24 hours prior to the drug docetaxel, for their ability to improve the efficiency of the drug docetaxel. Nanoparticles of poly(lactic- co -glycolic) acid (PLGA) with a hydrodynamic diameter of 63 nm, functionalized with galactosamine, showed efficient in vitro CYP3A4 inhibition and the highest accumulation in hepatocytes. When compared to docetaxel alone, in nude mice bearing the human breast cancer, MDA-MB-231 model, they significantly improved the delay in tumor growth (treated group versus docetaxel alone, percent treated versus control ratio [%T/C] of 32%) and demonstrated a major improvement in overall survival (survival rate of 67% versus 0% at day 55).

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Transferrin Adsorption onto PLGA Nanoparticles Governs Their Interaction with Biological Systems from Blood Circulation to Brain Cancer Cells

Abstract: Taken together, those data evidence that Tf-NPs represent an interesting nanomedicine to deliver anticancer drugs to glioma cells through systemic or locoregional strategies at early and late tumor stages.

Cited by 96 publications

References 65 publications

Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents

Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents

Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Nano-sized cytochrome P450 3A4 inhibitors to block hepatic metabolism of docetaxel

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Transferrin Adsorption onto PLGA Nanoparticles Governs Their Interaction with Biological Systems from Blood Circulation to Brain Cancer Cells

Abstract: Taken together, those data evidence that Tf-NPs represent an interesting nanomedicine to deliver anticancer drugs to glioma cells through systemic or locoregional strategies at early and late tumor stages.

Cited by 96 publications

References 65 publications

Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents

Use of Ferritin-Based Metal-Encapsulated Nanocarriers as Anticancer Agents

Development of polymeric&ndash;cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Nano-sized cytochrome P450 3A4 inhibitors to block hepatic metabolism of docetaxel

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Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery