Transferable Immunoglobulin A–Coated Odoribacter splanchnicus in Responders to Fecal Microbiota Transplantation for Ulcerative Colitis Limits Colonic Inflammation

Lima, Svetlana; Gogokhia, Lasha; Viladomiu, Monica; Chou, Li-Shan; Putzel, Gregory G.; Jin, Wenbing; Pires, Sílvia; Guo, Chun-Jun; Gerardin, Ylaine; Crawford, Carl V.; Jacob, Vinita; Scherl, Ellen; Brown, Su-Ellen; Hambor, John E.; Longman, Randy S.

doi:10.1053/j.gastro.2021.09.061

Cited by 64 publications

(52 citation statements)

References 45 publications

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“…Most studies suggest that the microbiota in recipients after FMT tends towards the donor composition [ 35 , 47 , 48 , 49 ]. Therefore, this test can also evaluate the success of FMT.…”

Section: Microbiological Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Microbiota Transplantation in an Antibiotic-Induced Bacterial Depletion Mouse Model: Reproducible Establishment, Analysis, and Application

Shang

Dai

et al. 2022

Microorganisms

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The fecal bacteria transplantation (FMT) technique is indispensable when exploring the pathogenesis and potential treatments for microbiota-related diseases. For FMT clinical treatments, there are already systematic guidelines for donor selection, fecal bacterial separation, FMT frequency, and infusion methods. However, only a few studies have demonstrated the use of standardized FMT procedures for animal models used in theoretical research, creating difficulties for many new researchers in this field. In the present paper, we provide a brief overview of FMT and discuss its contribution to the current understanding of disease mechanisms that relate to microbiota. This protocol can be used to generate a commonly used FMT mouse model and provides a literature reference of customizable steps.

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“…Most studies suggest that the microbiota in recipients after FMT tends towards the donor composition [ 35 , 47 , 48 , 49 ]. Therefore, this test can also evaluate the success of FMT.…”

Section: Microbiological Analysesmentioning

confidence: 99%

“…Researchers often combine FMT with other techniques to further elucidate the underlying mechanisms of FMT treatment of diseases. Using the sorting and sequencing of immunoglobulin (Ig) A-coated microbiota (called IgA-seq) techniques, Lima et al identified immune-reactive microbiota during FMT [ 49 ].…”

Section: Applicationmentioning

confidence: 99%

Microbiota Transplantation in an Antibiotic-Induced Bacterial Depletion Mouse Model: Reproducible Establishment, Analysis, and Application

Shang

Dai

et al. 2022

Microorganisms

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“…Fecal microbiota transplantation (FMT) refers to the transplantation of functional flora from healthy human feces into the intestinal tract of patients, rebuilding new intestinal flora, and thus treating diseases ( 17 ). Clinical trials have shown that FMT is effective for treating UC ( 18 , 19 ). There were also animal experiments to investigate the therapeutic mechanism of FMT in the treatment of IBD.…”

Section: Introductionmentioning

confidence: 99%

“…However, as yet, we do not understand the mechanism of action of FMT, and its causal relationship with the treatment of diseases ( 21 ). Further research is necessary to identify the microbes that are responsible for the therapeutic effect of FMT, in order to develop more effective microbiota based treatments for UC ( 18 , 22 ).…”

Section: Introductionmentioning

confidence: 99%

Human Fecal Microbiota Transplantation Reduces the Susceptibility to Dextran Sulfate Sodium-Induced Germ-Free Mouse Colitis

et al. 2022

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In clinical practice, fecal microbiota transplantation (FMT) has been used to treat inflammatory bowel disease (IBD), and has shown certain effects. However, the selection of FMT donors and the mechanism underlying the effect of FMT intervention in IBD require further exploration. In this study, dextran sodium sulfate (DSS)-induced colitis mice were used to determine the differences in the protection of colitis symptoms, inflammation, and intestinal barrier, by FMT from two donors. Intriguingly, pre-administration of healthy bacterial fluid significantly relieved the symptoms of colitis compared to the ulcerative colitis (UC) bacteria. In addition, healthy donor (HD) bacteria significantly reduced the levels of inflammatory markers Myeloperoxidase (MPO) and Eosinophil peroxidase (EPO), and various pro-inflammatory factors, in colitis mice, and increased the secretion of the anti-inflammatory factor IL-10. Metagenomic sequencing indicated higher species diversity and higher abundance of anti-inflammatory bacteria in the HD intervention group, including Alistipes putredinis, Akkermansia muciniphila, Bifidobacterium adolescentis, short-chain fatty acids (SCFAs)-producing bacterium Christensenella minuta, and secondary bile acids (SBAs)-producing bacterium Clostridium leptum. In the UC intervention group, the SCFA-producing bacterium Bacteroides stercoris, IBD-related bacterium Ruminococcus gnavus, Enterococcus faecalis, and the conditional pathogen Bacteroides caccae, were more abundant. Metabolomics analysis showed that the two types of FMT significantly modulated the metabolism of DSS-induced mice. Moreover, compared with the UC intervention group, indoleacetic acid and unsaturated fatty acids (DHA, DPA, and EPA) with anti-inflammatory effects were significantly enriched in the HD intervention group. In summary, these results indicate that FMT can alleviate the symptoms of colitis, and the effect of HD intervention is better than that of UC intervention. This study offers new insights into the mechanisms of FMT clinical intervention in IBD.

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“…FMT technology was first used for treating refractory Clostridium difficile infection in 2013[ 11 ]. FMT also aids in inducing the remission of ulcerative colitis and improving hepatic encephalopathy and insulin sensitivity[ 12 - 14 ]. Washed microbiota transplantation (WMT), the generation of bacterial solutions via automatic purification systems, is respected in clinic.…”

Section: Introductionmentioning

confidence: 99%

Washed microbiota transplantation reduces serum uric acid levels in patients with hyperuricaemia

Cai¹,

Chen²,

He³

et al. 2022

WJCC

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BACKGROUND Previous studies have found that hyperuricaemia (HUA) is closely related to intestinal flora imbalance. AIM The current study investigated the effects and safety of washed microbiota transplantation (WMT) on serum uric acid (SUA) levels in different populations. METHODS A total of 144 patients who received WMT from July 2016 to April 2020 in the First Affiliated Hospital of Guangdong Pharmaceutical University and had SUA data before treatment were selected. Changes in SUA levels before and after treatment were retrospectively reviewed based on short-term and mid-term effects of WMT regimens. SUA levels measured in the last test within 3 mo after the first WMT represented the short-term effect, and SUA levels measured in the last test within 3-6 mo after the first WMT represented the mid-term effect. The patients were divided into an HUA group (SUA > 416 μM) and a normal uric acid (NUA) group (SUA ≥ 202 μM to ≤ 416 μM) based on pretreatment SUA levels. RESULTS Average short-term SUA levels in the HUA group decreased after WMT (481.00 ± 99.85 vs 546.81 ± 109.64 μM, n = 32, P < 0.05) in 25/32 patients and returned to normal in 10/32 patients. The short-term level of SUA reduction after treatment moderately correlated with SUA levels before treatment ( r = 0.549, R ² = 0.300, P < 0.05). Average SUA levels decreased after the first and second courses of WMT (469.74 ± 97.68 vs 540.00 ± 107.16 μM, n = 35, and 465.57 ± 88.88 vs 513.19 ± 78.14 μM, n = 21, P < 0.05). Short-term and mid-term SUA levels after WMT and SUA levels after the first, second and third courses of WMT were similar to the levels before WMT in the NUA group ( P > 0.05). Only 1/144 patients developed mild diarrhea after WMT. CONCLUSION WMT reduces short-term SUA levels in patients with HUA with mild side effects but has no obvious effect on SUA levels in patients with NUA.

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Transferable Immunoglobulin A–Coated Odoribacter splanchnicus in Responders to Fecal Microbiota Transplantation for Ulcerative Colitis Limits Colonic Inflammation

Cited by 64 publications

References 45 publications

Microbiota Transplantation in an Antibiotic-Induced Bacterial Depletion Mouse Model: Reproducible Establishment, Analysis, and Application

Microbiota Transplantation in an Antibiotic-Induced Bacterial Depletion Mouse Model: Reproducible Establishment, Analysis, and Application

Human Fecal Microbiota Transplantation Reduces the Susceptibility to Dextran Sulfate Sodium-Induced Germ-Free Mouse Colitis

Washed microbiota transplantation reduces serum uric acid levels in patients with hyperuricaemia

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