Transferable cephalosporin resistance not inhibited by clavulanate in Escherichia coli

Woodford, Neil; Payne, D. J.; Johnson, A.P.; Weinbren, M.; Perinpanayagam, R.M.; George, R.C.; Cookson, Barry; Amyes, S. G. B.

doi:10.1016/0140-6736(90)91784-8

Cited by 20 publications

(6 citation statements)

References 5 publications

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“…The phenotype of our isolate, mainly considering the low level of resistance to moxalactam and temocillin, resembles those found in ampC E. coli P-lactamase-hyperproducing strains (18,24). The IC50s of clavulanic acid, cloxacillin, and aztreonam were also in agreement with those described for other plasmid-mediated AmpCtype P-lactamases (29,30,41,45).…”

Section: Discussionsupporting

confidence: 86%

Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants

Leiza

Pérez-Díaz

Ayala

et al. 1994

Antimicrob Agents Chemother

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Klebsiella pneumoniae BA32, a clinical isolate from Buenos Aires, Argentina, was found to produce a plasmid-encoded beta-lactamase (FOX-1) which conferred resistance to broad-spectrum cephalosporins and cephamycins. Resistance could be transferred by conjugation or transformation into Escherichia coli K-12 via a 48.5-kb plasmid (pGLK1) that produced two FOX-1 molecular variants with isoelectric points of 6.8 and 7.2 and apparent molecular sizes of 37 and 35 kDa, respectively. The kinetic study revealed that the two variants had very similar substrate and inhibition profiles. These values resemble those of chromosomally mediated class C (group 1) cephalosporinases. The structural gene of FOX-1 (blaFOX-1) was cloned into a 2,270-bp PstI-PstI fragment and was expressed in E. coli TG1. The deduced 382-amino-acid sequence of FOX-1 exhibited a high degree of similarity with chromosomally encoded AmpC beta-lactamases of Pseudomonas aeruginosa, Serratia marcescens, Enterobacter cloacae, E. coli, and Citrobacter freundii. These findings suggest that FOX-1 is a plasmid-mediated AmpC-type beta-lactamase that is encoded by a single gene and that has two molecular variants.

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Section: Discussionsupporting

confidence: 86%

Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants

Leiza

Pérez-Díaz

Ayala

et al. 1994

Antimicrob Agents Chemother

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“…Third, some plasmid-mediated extendedspectrum beta-lactamases are not inhibited by clavulanic acid. Although the number of strains producing such an enzyme is increasing, it is still small (20)(21)(22). It is therefore unlikely that our isolates would produce such an enzyme.…”

Section: Discussionmentioning

confidence: 95%

Beta-lactam resistance amongEscherichia coli andKlebsiella species blood culture isolates in Finnish hospitals

Arstila¹,

Auvinen

Huovinen³

1994

Eur. J. Clin. Microbiol. Infect. Dis.

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The aim of this study was to investigate beta-lactam resistance in Escherichia coli and Klebsiella spp. blood culture isolates in Finland. Special attention was given to extended-spectrum beta-lactamases. A total of 566 Escherichia coli and 108 Klebsiella spp. blood culture isolates were collected from hospitals throughout Finland and their susceptibility to beta-lactam antibiotics studied. Twenty percent of Escherichia coli and 69% of Klebsiella spp. strains were resistant to ampicillin. The mechanisms of resistance were studied by hybridization, isoelectric focusing and the clavulanate double-disk potentiation test. Of the ampicillin-resistant Escherichia coli strains, 83% produced TEM-1. Of the ampicillin-resistant Klebsiella spp. strains, 43% produced SHV-1. Only nine Escherichia coli and three Klebsiella spp. isolates were resistant to cefuroxime (MIC > or = 32 micrograms/ml), and none were resistant to third-generation cephalosporins. These data were compared with cefuroxime and third-generation cephalosporin consumption levels in Finnish hospitals. Although the use of cephalosporins is far more extensive in Finland than in other Scandinavian countries, none of the isolates produced extended-spectrum beta-lactamases. In conclusion, resistance to cefuroxime has remained rare in Finland, and cefuroxime is still an alternative to third-generation cephalosporins in the treatment of septicemia.

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“…Tests with high inocula lack interpretive criteria and can lead to false resistance. Double-disk tests can lack sensitivity because of problems of optimal disk spacing (24), the inability of clavulanate to inhibit all ESIs (2,16,26), and the inability of the test to detect ES,Bs in strains that also produce chromosomal cephalosporinases (5,8).…”

mentioning

confidence: 99%

Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests

Thomson

Sanders

1992

Antimicrob Agents Chemother

199

208

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The three-dimensional and clavulanate double-disk potentiation tests were compared as procedures for the detection of extended-spectrum 13-lactamase production in 32 strains of Escherichia coli and Klebsiella pneumoniae, 31 of which produced TEM-1, TEM-2, TEM-3, TEM-4, TEM-5, TEM-7, TEM-8, TEM-9, TEM-10, TEM-12, TEM-101, SHV-1, SHV-2, SHV-3, SHV-4, SHV-5, CAZ-2, MIR-1, or an unidentified extended-spectrum 13-lactamase with a pl of 5.95, with some strains producing multiple 13-lactamases. The three-dimensional test, which was performed in conjunction with a routine disk diffusion test, detected extended-spectrum 13-lactamase production in 26 of 28 (93%) of the strains that produced extended-spectrum 13-lactamases. The clavulanate double-disk potentiation test detected extended-spectrum 13-lactamases in only 22 of the 28 strains (79%o) when it was performed as currently recommended. The three-dimensional test, when performed in conjunction with the disk diffusion test, offered the advantages of providing simultaneous information about both antibiotic susceptibility and extended-spectrum 13-lactamase production, coupled with a greater sensitivity and earlier detection of extended-spectrum 13-lactamases.

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Transferable cephalosporin resistance not inhibited by clavulanate in Escherichia coli

Cited by 20 publications

References 5 publications

Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants

Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants

Beta-lactam resistance amongEscherichia coli andKlebsiella species blood culture isolates in Finnish hospitals

Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests

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