Transferable Antibiotic Resistance Elements in
            <i>Haemophilus influenzae</i>
            Share a Common Evolutionary Origin with a Diverse Family of Syntenic Genomic Islands

Mohd-Zain, Zaini; Turner, Sarah L.; Cerdeño-Tárraga, Ana; Lilley, Andrew K.; Inzana, Thomas J.; Duncan, A. J.; Harding, Rosalind M.; Hood, Derek W.; Peto, Tim E. A.; Crook, Derrick W.

doi:10.1128/jb.186.23.8114-8122.2004

Cited by 113 publications

(142 citation statements)

References 56 publications

(100 reference statements)

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“…As well as Haemophilus influenzae, discussed above, a number of organisms have been shown to harbour genomic islands which share genes and synteny with SPI-7: Xanthomonas axonopodis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Burkholderia xenovorans, Ralstonia metallidurans, Yersinia enterocolitica and Photorhabdus luminescens [5,15]. The level of amino acid identity is often very low, but core elements of the islands can be identified, including portions of the rep, tra and int regions (Figure).…”

Section: Evolutionary Origins Of Spi-7mentioning

confidence: 99%

“…There are also putative phage integrases (two in the S. Typhi island, and one in the S. Dublin and S. Paratyphi C islands) which may promote the excision and integration of the island. A genomic island from Haemophilus influenzae, ICEHin1056, has notable similarity to SPI-7 [15]. ICEHin1056 has been found to encode a novel Type IV Secretion System, which allows conjugation of the island [16].…”

Section: Mobility Of Spi-7mentioning

confidence: 99%

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SPI-7: Salmonella’s Vi-Encoding Pathogenicity Island

Seth-Smith

2008

J Infect Dev Ctries

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Salmonella Pathogenicity Island-7 (SPI-7) is a large, mosaic, genetic island, found in several serovars of Salmonella enterica subsp. enterica associated with systemic disease. As well as encoding genes which may aid its own transmission, it carries genes for potential virulence factors such as Vi antigen, SopE effector and type IVB pili. The stability of SPI-7 is of interest with respect to typhoid fever and related vaccines.

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Section: Evolutionary Origins Of Spi-7mentioning

confidence: 99%

Section: Mobility Of Spi-7mentioning

confidence: 99%

SPI-7: Salmonella’s Vi-Encoding Pathogenicity Island

Seth-Smith

2008

J Infect Dev Ctries

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“…The IS-left and -right of the class I transposon contained tnpA that have been found in STX MO10 of Vibrio cholerae (7), but IS-left was 5'-truncated by the invasion of IS26 found in Klebsiella pneumonia (20). The transposon carried a class II cat, tnpA and truncated tnpA of Tn10 found in Haemophilus influenzae (12), and the gene for a hypothetical protein of Aeromonas punctata (15). The sequence TAATAG found at the flanking region seemed to be a recognition site for this class I transposon.…”

mentioning

confidence: 99%

Structural Variation of Tn10 that Carries tetB Found in Fish Farm Bacteria

et al. 2011

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Three variants of the composite transposon Tn10 were extracted from transferable plasmids of fish farm bacteria. These variants were identical in insertions with IS10, but differed in another class I transposon insertion and a region of homologous recombination downstream of tetB.Key words: Plasmid, Tn10, fish farm bacteria Tn10 is a composite transposon consisting of IS10 and the tetB operon carrying a tetracycline resistance gene. Its complete nucleotide sequence is already well known (2) and its distribution has been reported for pathogenic bacteria, and for some bacteria with structural variations (11, 16). The structure of Tn10 on the transferable plasmid from Pantoea agglomerans is heterogeneous (16), and a vestige of Tn10 has been found in an integron on a plasmid from Salmonella enterica (11).The distribution of Tn10 has also been reported for marine bacteria (9), but the variation in Tn10 structure has not yet been well known. Therefore, we examined the structural variation of Tn10 on transferable tetB-carrying plasmids which have been found in fish farm bacteria (4).We isolated four fish farm bacteria harboring transferable tetB-carrying plasmids listed in Table 1 (4). Transconjugant strains were obtained by conjugation between the fish farm bacteria and E. coli HB-101 (Table 1) (4). All strains grew on Luria-Bertani (LB) medium with or without 20 µg mL −1 of tetracycline (TC) at 37°C. The plasmids were extracted from the transconjugants by the Kado and Liu method (8), and used as template DNA for PCR amplification of Tn10 regions with the primers listed in Table 2 and Fig. 1.Although most of the primer sets produced DNA fragments of predicted size in all PCR-amplifications, all of the products of primer set 2 were approximately 1,300 bp larger than the predicted size. Primer set 3 produced no PCR products for TC68 or TC69, and no product was obtained using primer set 7 or 8 for TC21 (Fig. 1). These non-amplifiable regions were cloned as shown in Table 1.Sequencing of the PCR product of primer set 2 indicated the presence of an insertion sequence within the jemA region (Fig. 1). Nucleotide sequences of the insertion and insertion point of all strains were identical. The insertion sequences were identified as an IS10 variant since they showed 87.4% similarity to the sequence of IS10 (2). The tnpA (transposase gene) sequence of the IS10 variant showed 91.1% similarity to the IS10 sequence present in the left and the right terminus of Tn10 of Shigella flexneri (Table 3). The repeat of ACCAAAGCC, flanking the inserted IS10 variant, was different from the typical target sequence of Tn10, NGCTNAGCN, by two nucleotides. ACCAAAGCC did not seem to be a new hot spot for IS10 variants, since the IS10 variant did not contain nucleotide substitutions at codons 134 and 249 that were reported to result in a relaxation of target specificity (1).In addition to the insertion of the identical IS10 variant in the jemA region, all terminal IS10s of the Tn10 variants were identical, except for IS10-right of TC21. The IS10-r...

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“…Estes genes "core" foram assim denominados por definirem uma subfamília de ilhas genômicas sintênicas consistindo de 33 genes presentes em ilhas genômicas de 16 espécies de β e γ-proteobactéria, indicando que estes elementos podem ter uma origem comum (Mohd-Zain, et al 2004). O conteúdo G+C desta região é 69,6 %, mais 32 alto que a média do genoma, que é de 64% (da Silva, et al 2002).…”

Section: Ilhas Na Linhagem Pa14unclassified

“…Finally, in order to verify interactions involving the conserved proteins in PAPI-1 and XAC, two hybrid assays were carried out, but only false positives results were obtained. (Koonin, et al 2001;Mohd-Zain, et al 2004;Juhas, et al 2007;Mathee, et al 2008;Battle, et al 2009;Sentchilo, et al 2009 …”

mentioning

confidence: 99%

Fatores envolvidos com a mobilização de PAPI-1

Stefanello¹

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Aos meus pais por me ensinaremque não existe recompensa sem luta, e por me apoiarem sempre. AGRADECIMENTOSAos meus pais Rudimar e Maria que me deram a vida, educação, puxões de orelha quando necessário e por respeitarem minhas decisões mesmo quando julgaram não ser a melhor escolha.A minha irmã Luiza, por me aturar durante seus 16 anos de vida. Te adoro maninha! À Dra Regina Baldini, pela grande paciência, dedicação e disponibilidade e simpatia durante a convivência enquanto este trabalho era desenvolvido.Aos amigos do grupo da professora Regina: Ana Paula, Ana Laura, Diogo, Gian, Gilberto e Patrícia por ajudarem sempre quando necessário e pelos momentos de descontração dentro e fora do laboratório.Aos amigos Feto, Sinistro, Valdir, Felipe, Seninha, Pererê, Guilherme e Rodrigo pelos ótimos momentos nas diversas repúblicas que morei.À Dra Suely Lopes Gomes pela disponibilidade do espaço físico e de materiais necessários para que esse trabalho fosse possível.Ao professor Dr Chuck Shaker Farah, por ceder a biblioteca de duplo-híbrido, aos alunos Maxuel, Cristiane e Claudia pelo auxilio com os ensaios.Ao pessoal do grupo da professora Suely: André, Anne, César, Cristian, Gabriela, Karina, Raphaela, Rogério, Michele pelas discussões e dicas de trabalho, conversas agradáveis e almoços no bandejão nosso de cada dia.As professoras, Dra Rita de Cássia Garcia Simão, pela orientação durante minha graduação como seu aluno de iniciação cientifica e por ter aberto as portas para eu chegar até aqui; a professora Dra Marina Kimiko Kadowaki e a professora Dra Clarisse Aoki Osaku por me dar as bases em Bioquímica e me emprestarem o material por onde estudei para a prova de ingresso no IQ-USP. Ao professor Dr Vladimir Pavan Margarido que me deu as bases de genética molecular e pelas longas horas de conversa sobre ciência entre outras coisas durante minha graduação. Sandra, Marivon, Luci, pelo trabalho de base além da paciência.Aos funcionários da secretaria de pós-graduação, Cibele, Emiliano e Milton pela atenção, ajuda e eficiência sempre que necessário.Aos meus grandes amigos do Paraná que sempre me aturaram e me fizeram feliz durante minha vida e que sempre faziam minha curta passagem por casa nos feriados bastante movimentada.A Dominice Lanzarini, com quem compartilhei alegrias, tristezas, frustrações e descobertas durante 3 excelentes anos da minha vida. Te respeitarei sempre.Ao CNPq e a FAPESP pelo apoio financeiro. Genomas bacterianos são extremamente dinâmicos e boa parte dessa dinâmica ocorre devido a transferência horizontal e aquisição de DNA exógeno, um processo natural e fundamental para a evolução, adaptação e diversificação dos microrganismos. Ilhas genômicas são grandes regiões do cromossomo bacteriano adquiridas por transferência horizontal e estão presentes em apenas algumas linhagens, podendo conferir alguma vantagem adaptativa. Em Pseudomonas aeruginosa, até o momento foram caracterizadas pelo menos dezesseis ilhas genômicas e cada uma delas confere características diferentes a seus hospedeiros. Em P. aeruginos...

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Transferable Antibiotic Resistance Elements in Haemophilus influenzae Share a Common Evolutionary Origin with a Diverse Family of Syntenic Genomic Islands

Cited by 113 publications

References 56 publications

SPI-7: Salmonella’s Vi-Encoding Pathogenicity Island

SPI-7: Salmonella’s Vi-Encoding Pathogenicity Island

Structural Variation of Tn10 that Carries tetB Found in Fish Farm Bacteria

Fatores envolvidos com a mobilização de PAPI-1

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