Transferability and reproducibility of exposed air-liquid interface co-culture lung models

Braakhuis, Hedwig; Gremmer, Eric R.; Bannuscher, Anne; Drašler, Barbara; Keshavan, Sandeep; Rothen‐Rutishauser, Barbara; Birk, Barbara; Verlohner, Andreas; Landsiedel, Robert; Meldrum, Kirsty; Doak, Shareen H.; Clift, Martin J. D.; Erdem, Johanna Samulin; Foss, Oda A H; Zienolddiny, Shanbeh; Serchi, Tommaso; Moschini, Elisa; Weber, Patricia A.; Burla, Sabina; Kumar, Pramod; Schmid, Otmar; Zwart, Edwin; Vermeulen, Jolanda P.; Vandebriel, Rob J.

doi:10.1016/j.impact.2023.100466

Cited by 12 publications

(2 citation statements)

References 57 publications

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“…Calu-3 cells, cultivated at the air interface, are a highly useful cellular mucus model for the investigation of delivery systems intended for pulmonary use ( Sonntag et al, 2022 ). Commercial availability and tight junction formation are the advantages of the Calu-3 cell line as an in vitro model ( Braakhuis et al, 2023 ). In particular, mucus production when cultured at air interface conditions, is an important benefit of the use of Calu-3 cell line, compared to other commonly used airway epithelium cell lines ( Ghanem et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Meziu,

Shehu,

Koch

et al. 2023

International Journal of Pharmaceutics: X

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Section: Resultsmentioning

confidence: 99%

Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Meziu,

Shehu,

Koch

et al. 2023

International Journal of Pharmaceutics: X

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“…On the other hand, ALI cell culture models provide many advantageous characteristics over traditional submerged cell culture conditions regarding reproduction of in vivo pulmonary host-defence interactions, lung physiology and accurate measurement of deposited dose 10 , 12 , 58 - 63 . For instance, bronchial epithelium grown and differentiated into stratified cells (basal cells, goblet cells, ciliated cells) at the ALI develop cilia, can form tight junctions, and can release mucin as well as pro- and anti-inflammatory mediators 64 , 65 .…”

Section: Current Models For Inhalation Toxicity Assessment Of Tobacco...mentioning

confidence: 99%

Challenges in current inhalable tobacco toxicity assessment models: A narrative review

Muthumalage,

Noel,

Thanavala

et al. 2024

Tob. Induc. Dis.

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Development and characterisation of a novel complex triple cell culture model of the human alveolar epithelial barrier

Mitchell,

Meldrum,

Bateman

et al. 2024

In vitro models

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Owing to increased pressure from ethical groups and the public to avoid unnecessary animal testing, the need for new, responsive and biologically relevant in vitro models has surged. Models of the human alveolar epithelium are of particular interest since thorough investigations into air pollution and the effects of inhaled nanoparticles and e-cigarettes are needed. The lung is a crucial organ of interest due to potential exposures to endogenous material during occupational and ambient settings. Here, an in vitro model of the alveolar barrier has been created in preparation for use in the quasi-air liquid interface (qALI) and (aerosol) air–liquid interface (ALI) exposures. The model consists of an alveolar type 1-like cell line (TT1), an alveolar type 2-like cell line (NCI-H441) and a model of (alveolar) macrophages (dTHP-1). The model formulates a complex, multi-cellular system, cultured at the air–liquid interface, that mimics the apical layer of the alveolar epithelial region in the human lung. Characterisation data has shown that both TT1 and NCI-H441 epithelial cells are able to be cultured together in addition to dTHP-1 cells through imaging (morphology), pro-inflammatory response and viability measurements. This dataset also demonstrates evidence of a reasonable barrier created by the cell culture in comparison to negative controls. Furthermore, it shows that while maintaining a low baseline of (pro)-inflammatory mediator expression during normal conditions, the model is highly responsive to inflammatory stimuli. This model is proposed to be suitable for use in toxicology testing of inhaled exogenous agents.

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Transferability and reproducibility of exposed air-liquid interface co-culture lung models

Cited by 12 publications

References 57 publications

Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Challenges in current inhalable tobacco toxicity assessment models: A narrative review

Development and characterisation of a novel complex triple cell culture model of the human alveolar epithelial barrier

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