Transfer-Printing of Highly Aligned DNA Nanowires

Nakao, Hidenobu; Gad, M.; Sugiyama, Shigeru; Otobe, Kazunori; Ohtani, Toshio

doi:10.1021/ja034185w

Cited by 107 publications

(92 citation statements)

References 29 publications

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“…The DNA nanostrands can be transferred onto other solid surfaces by contact printing (17,18,20). For short nanostrands, we found that the stretched DNA molecules broke at the edges of the microwells, resulting in portions in the microwells being untransferred.…”

Section: Discussionmentioning

confidence: 98%

“…A line-patterned polydimethyl siloxane (PDMS) stamp, which contained a positively charged surface as a substrate for combing, also produced an array of stretched DNA molecules (17). The stretched DNA molecules could be transferred onto other solid surfaces by contact printing, allowing for the generation of more complex patterns (17,18). However, the position and length of the DNA molecules in the arrays prepared by these two methods could not be tightly controlled.…”

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confidence: 99%

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Generating highly ordered DNA nanostrand arrays

Guan

Lee²

2005

Proc. Natl. Acad. Sci. U.S.A.

132

129

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Highly ordered arrays of stretched DNA molecules were generated over the millimeter scale by using a modified molecular combing method and soft lithography. Topological micropatterning on polydimethyl siloxane stamps was used to mediate the dynamic assembly of DNA molecules into arranged nonostrand arrays. These arrays consisted of either short nanostrands of several micrometers with fixed length and orientation or long nanostrands up to several hundred micrometers in length. The nanostrand arrays were transferred onto flat solid surfaces by contact printing, allowing for the creation of more complex patterns. This technique has potential applications for the construction of next-generation DNA chips and functional circuits of DNA-based 1D nanostructures. molecular combing ͉ soft lithography P atterning DNA molecules at the micrometer scale forms the basis of DNA chips, a widely used technology for genetic analysis and diagnosis (1, 2). At the molecular level, single DNA molecules have been stretched for physical mapping of genes and molecular diagnosis of diseases (3). If stretched DNA molecules can be patterned into a well defined array, large-scale and highly automated analysis may be realized. On the other hand, 1D nanostructures are of great interest for the construction of future devices (4-6). With its high aspect ratio, unique base-pairing ability, designable base sequence, and availability of various techniques for functionalization, DNA is a very attractive material for preparing 1D nanostructures for electronic, magnetic, photonic, and chemical sensing applications (7-13). The ability to position a large number of 1D nanostructures with well defined linear arrangements is a prerequisite for integrating them into functional devices. The lack of this ability is currently hindering the realization of functional devices built on DNAbased 1D nanostructures.Molecular combing is a technique for stretching, aligning, and immobilizing coiled DNA molecules in a solution onto a f lat surface through a dewetting process (3). By creating a pattern of surface structures or properties, combing can be further controlled (14 -18). A number of studies in molecular combing have been reported in the literature, but none are able to demonstrate well defined arrays. For example, a single nanofiber has been combed and placed between two electrodes in a nanojunction (14). But this method cannot control the size of nanofibers and has not demonstrated any ability to pattern nanofiber arrays covering a large area. By f lowing DNA solution through microchannels, stretched DNA molecules confined in the microchannels were obtained. Their orientation and curvature were directed by controlling the geometry of the air-water interface (15). However, the DNA molecules were randomly distributed in the microchannels. In a separate study, polystyrene lines were lithographically patterned on a substrate for end-specific binding of DNA molecules. Combing of the DNA on this substrate created lines of stretched single DNA molecules (16). A li...

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Generating highly ordered DNA nanostrand arrays

Guan

Lee²

2005

Proc. Natl. Acad. Sci. U.S.A.

132

129

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“…[111] The authors stress that biological assemblies of nanocolloids may play an important role in the preparation of linear NP chains due to the opportunities that are opening up with regard to biological coding of the assembly process. Recent research has demonstrated that 1D NP chains could be accurately positioned and assembled into complex patterns by DNA templates on solid substrates, [112,113] which also can be utilized in the practical fabrication of NP devices.…”

Section: Preparation Methodsmentioning

confidence: 99%

One‐Dimensional Assemblies of Nanoparticles: Preparation, Properties, and Promise

Tang

Kotov²

2005

Advanced Materials

763

602

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Nanoparticle (NP) assemblies are of considerable interest for both fundamental research and applications, since they provide direct bridges between nanometer‐scale objects and the macroscale world. Unlike two‐dimensional or three‐dimensional NP assemblies, which have been extensively studied and reviewed, reports on one‐dimensional (1D) NP assemblies are rather rare, even though these assemblies are likely to play critical roles in the improvement of the efficiencies of various electronic, optoelectronic, magnetic, and other devices based on single NPs or their composites. Additionally, 1D assemblies of NPs, i.e., chains, can significantly help in the understanding of a number of biological processes and fundamental quantum mechanics of nanometer‐scale systems. The difficulties are very evident when one wants to realize anisotropic 1D assemblies from presumably isotropic, zero‐dimensional NPs. In this context, the authors present a systemic review of current research on 1D NP assemblies. Their preparation methods are classified and novel characteristics of NP chains, such as collective properties and directional transfer of photons, electrons, spins, etc., are elucidated. Current problems underlying the fundamental research and practical applications of 1D NP assemblies are also addressed.

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“…Since a single k-DNA has a physical length of 16.5 lm and a typical diameter of ca. 0.4 nm in AFM images, [18] it is likely that the long and smooth nanowires were composed of multiple stretched DNA molecules. The dotlike microstructures in the fluorescence microscopy image are also shown in the AFM image.…”

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confidence: 99%

Forming Highly Ordered Arrays of Functionalized Polymer Nanowires by Dewetting on Micropillars

Guan

Lee

2007

Advanced Materials

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1D nanostructures, characterized by their extremely small lateral size, large surface-to-volume ratio, and quantum confinement effect, hold tremendous potential to build next-generation electronic circuits, sensors with high sensitivity and reaction rate, and even new devices operating on quantummechanical principles. [1][2][3] Polymers are intrinsic 1D nanostructures with well-defined and versatile structures and compositions. The lateral size of a polymer chain is generally below 10 nm, which is the limit of current lithographic technology and is also the critical size at which quantum-mechanical effects can become prominent. [2,4,5] DNA is by far the most extensively studied polymer as 1D nanomaterial due to its high aspect ratio, base-pairing capability, designable base sequence, chemical modifiability, and intriguing electronic properties.[6] It has been used as a template to prepare various types of functional nanowires by conjugation of a broad range of materials such as metals, [2,7] conducting polymers, [8] nanoparticles, [9,10] fluorophores, [11] and single-walled carbon nanotubes. [12] In addition to DNA, functional polymers such as conducting polymers hold potential to be directly used as 1D nanometer-sized building blocks to construct novel devices such as molecular circuits. Polymer chains generally adopt a coiled conformation in a solvent. In order to form 1D nanostructures and integrate them into a functional system, these coils need to be stretched and patterned into appropriate architectures. Examples of such architectures are suspended DNA-templated nanowire pairs in a quantum interference device [2] and crossbar arrays as address decoders.[13] Molecular combing and its derivatives are a set of methods capable of uncoiling, aligning, and immobilizing single molecules or nanowires utilizing the surface tension of water, but they are limited in the precise control of length and location of the stretched molecules over a large area. [14] Arrays of polymeric nanofibers can also be generated by electrospinning on a rotating substrate [15] or mechanical drawing, [16] but the electrospinning method cannot position the nanofibers precisely and the fibers produced by the mechanical drawing are over 50 nm in diameter. We developed a molecular-combing-based approach able to generate a highly ordered array of short (5-10 lm) and long (up to 1 mm) nanowires composed of stretched DNA molecules through dewetting of a DNA solution on a poly(dimethyl siloxane) (PDMS) stamp containing microwells on the surface. [17] In this paper,we report the use of a different type of surface features, micropillars, to generate arrays of DNA nanowires up to 1 cm in length, less than 10 nm in lateral size, and covering an area of up to 1 cm × 1 cm. We have also extended this method to several other polymers and demonstrated functionalization of these nanowires with small molecules and nanoparticles. As schematically shown in Figure 1, the generation of the polymer 1D nanowires was easily achieved by gently placing a PDMS stamp ...

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Transfer-Printing of Highly Aligned DNA Nanowires

Cited by 107 publications

References 29 publications

Generating highly ordered DNA nanostrand arrays

Generating highly ordered DNA nanostrand arrays

One‐Dimensional Assemblies of Nanoparticles: Preparation, Properties, and Promise

Forming Highly Ordered Arrays of Functionalized Polymer Nanowires by Dewetting on Micropillars

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