Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L.

Abstract

An introgression breeding programme was carried out to transfer the virus resistance gene AV-1pro from the wild species Asparagus prostratus to the garden asparagus Asparagus officinalis. Serious crossing barriers caused by genetic distance and different ploidy levels of the crossing parents have been overcome using embryo rescue for the F1, BC1, and BC2 generations. The male and female fertility was widely restored in BC2 and was shown to be comparable to the cultivated asparagus. Five AV-1 resistant diploid (2n = 2x = 20) BC2 plants were selected and reciprocally backcrossed with asparagus cultivars. Segregation analyses of fourteen seedborne BC3 progenies suggested a monogenic dominant inheritance of the AV-1 resistance. Genotyping by sequencing analysis gave a strong hint for location of the resistance gene on asparagus Chromosome 2. Using an Axiom single nucleotide polymorphism (SNP) genotyping array for the analysis of three BC3 families with 10 AV-1 resistant and 10 AV-1 susceptible plants each, as well as 25 asparagus cultivars, the AV-1pro locus on Chromosome 2 was further narrowed down. The SNP with the highest LOD score was converted to a kompetitive allele specific PCR (KASP) marker, shown to be useful for the further backcross programme and serving as the starting point for the development of a diagnostic marker.

The garden asparagus (Asparagus officinalis L.) mitochondrial genome revealed rich sequence variation throughout whole sequencing data

“…Garden asparagus ( Asparagus officinalis L.) is the most traditionally significant and best-known plant in Asparagaceae owing to its economical, nutritional, and medical value ( Norup et al., 2015 ; Zhang et al., 2022 ; García et al., 2023 ). Over the past 20 years, the acreage of this plant has expanded significantly worldwide, especially in China ( Nothnagel et al., 2022 ). At present, the main commercial asparagus varieties are diploid varieties that originate from the original “Violet Dutch.” ( Knaflewski, 1996 ).…”

“…Therefore, expanding the genetic diversity is a prominent goal in asparagus breeding. Interspecies hybridization is an important method for enriching genetic diversity and cultivating new cultivars ( Takeuchi et al., 2018 ; Moreno-Pinel et al., 2021 ; García et al., 2022 ; Nothnagel et al., 2022 ). However, at present, the genetic relationship among many plants in the genus Asparagus remains unknown, which has hindered advances in the genetics and breeding of A. officinalis ( Kubota et al., 2012 ; Mousavizadeh et al., 2021 ; García et al., 2022 ).…”

Genetic characterization of downy mildew resistance from the hop (Humulus lupulus L.) line USDA 64035M

“…The successful implementation of MAS for disease resistance is dependent on knowledge of the genetic architecture of host disease resistance. Studies have shown that MAS is most suitable for vertical resistance, which is often monogenic or oligogenic, while GS might be more suitable for quantitative or horizontal disease resistance (Miedaner & Korzun, 2012;Olatoye et al, 2019). Therefore, deciphering the genetic architecture of host resistance to a particular pathogen is essential for informed decision-making in MAB.…”

DataSheet_1.xlsx

“…Garden asparagus (Asparagus officinalis L.) is the most traditionally significant and best-known plant in Asparagaceae owing to its economical, nutritional, and medical value (Norup et al, 2015;Zhang et al, 2022;Garcıá et al, 2023). Over the past 20 years, the acreage of this plant has expanded significantly worldwide, especially in China (Nothnagel et al, 2022). At present, the main commercial asparagus varieties are diploid varieties that originate from the original "Violet Dutch."…”

“…Therefore, expanding the genetic diversity is a prominent goal in asparagus breeding. Interspecies hybridization is an important method for enriching genetic diversity and cultivating new cultivars (Takeuchi et al, 2018;Moreno-Pinel et al, 2021;Garcıá et al, 2022;Nothnagel et al, 2022). However, at present, the genetic relationship among many plants in the genus Asparagus remains unknown, which has hindered advances in the genetics and breeding of A. officinalis (Kubota et al, 2012;Mousavizadeh et al, 2021;Garcıá et al, 2022).…”