Transfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paper

Frossard, Philippe; Sisco, Kenneth L.; Rucknagel, Donald L.

doi:10.1016/0003-2697(83)90295-6

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…292 Currently, the Southern blot is used for the detection of PCR products and determination of the reaction specificity. 293 ± 295 Nitrocellulose filters 225,292,296,297 and nylon membranes 298,299 (less frequently, p-azidobenzoylcellulose 236 ) or dried gels following fractionation of NA fragments 300 are commonly used for the fixation of the nucleotide material. Sandwich blot is a promising approach to the analysis of NA in non-purified specimens containing proteins, polysaccharides and other components.…”

Section: Hybridisation Assaymentioning

confidence: 99%

Affinity sorbents containing nucleic acids and their fragments

Shishkina¹,

Левина²,

Зарытова³

2001

Russ. Chem. Rev.

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The published data on the main methods for the The published data on the main methods for the preparation of polymeric supports containing nucleic acids (NA) preparation of polymeric supports containing nucleic acids (NA) or their fragments (oligonucleotides) are reviewed with special or their fragments (oligonucleotides) are reviewed with special emphasis on chemical immobilisation. Some physical and phys-emphasis on chemical immobilisation. Some physical and physicochemical immobilisation techniques, including those based on icochemical immobilisation techniques, including those based on the use of enzymes and avidin ± biotin interactions and prepara-the use of enzymes and avidin ± biotin interactions and preparation of NA-containing supports by direct oligonucleotide syn-tion of NA-containing supports by direct oligonucleotide synthesis on these supports are considered. A special section is thesis on these supports are considered. A special section is devoted to the application of NA-containing sorbents for the devoted to the application of NA-containing sorbents for the isolation of individual NA and proteins as well as in hybridisation isolation of individual NA and proteins as well as in hybridisation analysis including those utilising DNA chips and DNA biosen-analysis including those utilising DNA chips and DNA biosensors. The bibliography includes 391 references sors. The bibliography includes 391 references. .

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