Transfer of Rhodocyclus gelatinosus to Rubrivivax gelatinosus gen. nov., comb. nov., and Phylogenetic Relationships with Leptothrix, Sphaerotilus natans, Pseudomonas saccharophila, and Alcaligenes latus

Willems, Anne; Gillis, Monique; Ley, J. De

doi:10.1099/00207713-41-1-65

Cited by 94 publications

(66 citation statements)

References 34 publications

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“…9) Only difference was observed in the use of carbon sources: the KDDS1 could not use butyrate as a carbon source. In addition, 16S rDNA sequence analysis revealed that the KDDS1 has 99.81% high homology to that of the type culture.…”

Section: Resultsmentioning

confidence: 99%

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Purification and Characterization of Alkaline Serine Proteinase from Photosynthetic Bacterium,Rubrivivax gelatinosusKDDS1

Oda

Tanskul

Oyama

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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In order to reduce the protein content of wastewater, photosynthetic bacteria producing proteinases were screened from wastewater of various sources and stocked in culture. An isolated strain, KDDS1, was identified as Rubrivivax gelatinosus, a purple nonsulfur bacterium that secretes proteinase under micro-aerobic conditions under light at 35 C. Molecular weight of the purified enzyme was estimated to be 32.5 kDa. The enzyme showed the highest activity at 45 C and pH 9.6, and the activity was completely inhibited by phenylmethyl sulfonyl fluoride (PMSF), but not by EDTA. The amino-terminal 24 amino acid sequence of the enzyme showed about 50% identity to those of serine proteinases from Pseudoalteromonas piscicida strain O-7 and Burkholderia pseudomallei. Thus, the enzyme from Rvi. gelatinosus KDDS1 was thought to be a serine-type proteinase. This was the first serine proteinase characterized from photosynthetic bacteria.

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Section: Resultsmentioning

confidence: 99%

“…gelatinosus has recently been renamed from Rhodocyclus gelatinosus. 9) In addition, the evolutionary tree between Rvi. gelatinosus KDDS1 and other bacteria showed that Rvi.…”

Section: Resultsmentioning

confidence: 99%

Purification and Characterization of Alkaline Serine Proteinase from Photosynthetic Bacterium,Rubrivivax gelatinosusKDDS1

Oda

Tanskul

Oyama

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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“…Data were obtained from Busse & Auling (1992), Kalmbach et al (1999), Malmqvist et al (1994), Moreira et al (2000), Palleroni & Palleroni (1978), Spring et al (1996), Takeda et al (2002) and Willems et al (1991) and Aquabacterium and the species Alcaligenes latus, is the temperature range of growth. Strain N2-680 T differs from species of the genus Leptothrix in the absence of a cell sheath and the inability to produce manganese oxides, as is typical for these organisms (Siering & Ghiorse, 1996).…”

Section: Resultsmentioning

confidence: 99%

“…Bacteriochlorophyll-containing members of this group are represented by the species Rubrivivax gelatinosus (Willems et al, 1991) and Roseateles depolymerans (Suyama et al, 1999). Rubrivivax gelatinosus comprises phototrophic non-sulphur bacteria that occur frequently in sewagetreatment plants and lagoons (Siefert et al, 1978;Pfennig, 1978;Willems et al, 1991). Roseateles depolymerans, isolated from river water in Japan, is an obligately aerobic, heterotrophic organism that produces bacteriochlorophyll a and carotenoid pigments only in the presence of low levels of carbon sources.…”

Section: Introductionmentioning

confidence: 99%

Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge

Manaia

Nunes

Nogales

2003

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain, N2-680 T (=DSM 15264 T =LMG 21760 T ), isolated from a thermophilic aerobic digester of municipal sludge, was characterized with respect to its morphology, physiology and taxonomy. Phenotypically, the isolate was a Gram-negative rod with a polar flagellum, catalase-and oxidase-positive, containing cytoplasmic inclusions of poly-b-hydroxybutyrate and had an optimal growth temperature of about 47 6C. Strain N2-680 T was unable to reduce nitrate and could use organic acids, amino acids and carbohydrates as single carbon sources. Chemotaxonomic analysis revealed that ubiquinone 8 was the major respiratory quinone of this organism and that phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. At 50 6C, the major components in fatty acid methyl ester analysis were C 16 : 0 and cyclo-C 17 : 0 . The highest 16S rDNA sequence identity of isolate N2-680 T was to Leptothrix mobilis and Ideonella dechloratans (95?7 %) and to Rubrivivax gelatinosus and Aquabacterium commune (95?6 %). 16S rDNA sequence similarities to species of two related thermophilic genera, Caldimonas manganoxidans and Tepidimonas ignava, were lower (93?6 and 94?7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-680 T represents a new genus and species, for which the name Caenibacterium thermophilum gen. nov., sp. nov. is proposed.

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“…For DNA-DNA binding experiments (see below), DNA was extracted from 5 to 10 g (wet weight) of cells grown in Roux flasks containing 130 ml of nutrient agar for 48 h at 30°C by using a slight modification of the method of Marmur (21), as described previously (36). For the amplified rDNA restriction analysis (ARDRA) (see below), total genomic DNA was purified by a method slightly modified from the method of Pitcher et al (26); cells grown in petri dishes containing nutrient agar for 24 to 48 h at 30°C were lysed in an additional step by incubating them with lysozyme at a final concentration of 50 mg/ml of TE buffer (10 mM Tris-HC1, 1 mM EDTA; pH 8.0) for 30 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Paenibacillus (Formerly Bacillus) gordonae (Pichinoty et. al. 1986) Ash et al. 1994 Is a Later Subjective Synonym of Paenibacillus (Formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: Emended Description of P. validus

Heyndrickx¹,

Vandemeulebroecke²,

Scheldeman³

et al. 1995

International Journal of Systematic Bacteriology

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A polyphasic study in which we performed an amplified ribosomal DNA restriction analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, a gas chromatographic analysis of methylated fatty acids, pyrolysis mass spectrometry, a random amplified polymorphic DNA analysis, a phenotypic analysis, and an analysis of the levels of DNA binding of Paenibacillus gordonae and Paenibacillus validus strains (including both type strains) showed that these organisms form a homogeneous group and that the names P.gordonae and P. validus are therefore synonyms. P. validus has nomenclatural priority, and an emended description of this species is given; the type strain is strain LMG 11161 (= ATCC 43897).

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Transfer of Rhodocyclus gelatinosus to Rubrivivax gelatinosus gen. nov., comb. nov., and Phylogenetic Relationships with Leptothrix, Sphaerotilus natans, Pseudomonas saccharophila, and Alcaligenes latus

Cited by 94 publications

References 34 publications

Purification and Characterization of Alkaline Serine Proteinase from Photosynthetic Bacterium,Rubrivivax gelatinosusKDDS1

Purification and Characterization of Alkaline Serine Proteinase from Photosynthetic Bacterium,Rubrivivax gelatinosusKDDS1

Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge

Paenibacillus (Formerly Bacillus) gordonae (Pichinoty et. al. 1986) Ash et al. 1994 Is a Later Subjective Synonym of Paenibacillus (Formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: Emended Description of P. validus

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