Transfer of Pseudomonas perfectomarina Baumann, Bowditch, Baumann, and Beaman 1983 to Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

Abstract

We propose the transfer of Pseudomonas perfectomarina to the species Pseudomonas stutzeri as strain ZoBell.The proposal is based on 60% deoxyribonucleic acid (DNA)-DNA homology with P . stutzeri ATCC 1758ST, a similar guanine-plus-cytosine content, and the conformity of more than 100 nutritional and general characteristics.

Taxonomic Relationship between Pseudomonas perfectomarina ZoBell and Pseudomonas stutzeri

“…Like all of the strains belonging to genomovar 2, strain ZoBell was able to grow on ethylene glycol and D-malate as sole carbon and energy sources and was not able to grow at 42°C and pH 6; it also was not able to use xylose and 4-aminobutyrate as sole carbon and energy sources. However, in contrast to all other strains belonging to genomovar 2, strain %Bell was not able to use mannitol, in agreement with previously published observations (5). Like most of the genomovar 2 strains, strain ZoBell was able to grow with fructose and not able to grow with suberate as sole carbon and energy sources.…”

“…This was confirmed by our characterization of the genomic structure of the species with genomovars (12). ' When P. perfectomarina was transferred to P. stutzeri, low levels of DNA-DNA homology with the type strain, ATCC 1.7588 (60%), and strain DSM 50227 (61%) were observed (5). If the genomovar subdivision of the species is used, this observation means that strain ZoBell does not belong to genomovar 1 (which contains the type strain) or genomovar * Corresponding author.…”

Isoprenoid quinone composition of some marine Alteromonas, Marinomonas, Deleya, Pseudomonas and Shewanella species

“…In this study, members of the genera could not be separated by quinone analysis (Table 2). Ubiquinone-8 has been found in species of Xanthomonas, Hydrogenophaga, Alcaligenes, Bordetella, Comamonas, Variouorax, Fruteuria and Moraxella (Yamada et al, 1982;Fletcher et al, 1987;Yamada et al, 1969;Nishimura et al, 1986), and as such is widely distributed in the rRNA superfamilies I, I1 and I11 that correspond to the /? and y subdivisions in the class Proteobacteria.…”

“…However, the quinone profile for this strain is the same as those of authentic Pseudomonas (Y amada et al, 1982 ;Oyaizu & Komagata, 1983). Pseudomonas stutzeri IAM 1293 1 was previously P. perfectomarina and was reassigned to this species on the basis of DNA-DNA hybridization studies (Dohler et al, 1987). Recently, Rossello et al (1991) reported that Pseudomonas stutzeri contains several genomic groups and clearly, further investigations of this group are required.…”

Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri

“…P. stutzeri ZoBell (formerly Pseudomonas perfectomarina ATCC 14405 [13]) was grown anaerobically in nitrate medium as described previously (12) but with the modification of the culture mode to a fed-batch procedure; aeration cycles were omitted. After 24, 44, and 54 h, sodium nitrate was added to the cultures to a final concentration of 1 g/liter.…”