Transfer of plasmid pJP4 from Escherichia coli and Pseudomonas putida to bacteria in activated sludge developed under different sludge retention times

Tsutsui, Hirofumi; Anami, Yasutaka; Matsuda, Masami; Inoue, Daisuke; Sei, Kazunari; Soda, Satoshi; Ike, Michihiko

doi:10.1016/j.jbiosc.2010.07.009

Cited by 21 publications

(12 citation statements)

References 26 publications

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“…identified in this study, especially P. putida can be specifically suggested as a reservoir of antimicrobial resistance, which is in agreement with the results of Meireles et al ( 2013 ). Also, Tsutsui et al ( 2010 ) suggested that the plasmid pJP4 was preferentially transferred to members of Pseudomonas spp., although longer sludge retention time increased phenotypical diversity among activated sludge transconjugants. Sludge retention time was also suggested to influence resistance rate among enteric bacteria (Luczkiewicz et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone

Łuczkiewicz

Kotlarska

Artichowicz

et al. 2015

Environ Sci Pollut Res

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In this study, species distribution and antimicrobial susceptibility of cultivated Pseudomonas spp. were studied in influent (INF), effluent (EFF), and marine outfall (MOut) of wastewater treatment plant (WWTP). The susceptibility was tested against 8 antimicrobial classes, active against Pseudomonas spp.: aminoglycosides, carbapenems, broad-spectrum cephalosporins from the 3rd and 4th generation, extended-spectrum penicillins, as well as their combination with the β-lactamase inhibitors, monobactams, fluoroquinolones, and polymyxins. Among identified species, resistance to all antimicrobials but colistin was shown by Pseudomonas putida, the predominant species in all sampling points. In other species, resistance was observed mainly against ceftazidime, ticarcillin, ticarcillin-clavulanate, and aztreonam, although some isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas pseudoalcaligenes, and Pseudomonas protegens showed multidrug-resistance (MDR) phenotype. Among P. putida, resistance to β-lactams and to fluoroquinolones as well as multidrug resistance become more prevalent after wastewater treatment, but the resistance rate decreased in marine water samples. Obtained data, however, suggests that Pseudomonas spp. are equipped or are able to acquire a wide range of antibiotic resistance mechanisms, and thus should be monitored as possible source of resistance genes.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone

Łuczkiewicz

Kotlarska

Artichowicz

et al. 2015

Environ Sci Pollut Res

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“…These factors include plasmid host range (61), sludge retention times (62), stressful environmental conditions (53), and host/donor phylogenetic affiliations (63). Significant enrichment of the ratios of multiple ARGs compared to 16S rRNA genes throughout the wastewater treatment process confirms the potential for mobility and proliferation of ARGs within the activated sludge microbial communities in full-scale treatment systems (64).…”

Section: Discussionmentioning

confidence: 99%

Fate and Persistence of a Pathogenic NDM-1-Positive Escherichia coli Strain in Anaerobic and Aerobic Sludge Microcosms

Mantilla‐Calderon

Hong

2017

Appl Environ Microbiol

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The presence of emerging biological pollutants in treated wastewater effluents has gained attention due to increased interest in water reuse. To evaluate the effectiveness of the removal of such contaminants by the conventional wastewater treatment process, the fate and decay kinetics of NDM-1-positive Escherichia coli strain PI7 and its plasmid-encoded antibiotic resistance genes (ARGs) were assessed in microcosms of anaerobic and aerobic sludge. Results showed that E. coli PI7 decayed at a significantly lower rate under anaerobic conditions. Approximate half-lives were 32.4 ± 1.4 h and 5.9 ± 0.9 h in the anaerobic and aerobic microcosms, respectively. In the aerobic microcosms, after 72 h of operation, E. coli PI7 remained detectable, but no further decay was observed. Instead, 1 in every 10,000 E. coli cells was identified to be recalcitrant to decay and persist indefinitely in the sludge. ARGs associated with the E. coli PI7 strain were detected to have transferred to other native microorganisms in the sludge or were released to the liquid fraction upon host decay. Extracellular DNA quickly degraded in the liquid fraction of the aerobic sludge. In contrast, no DNA decay was detected in the anaerobic sludge water matrix throughout the 24-h sampling period. This study suggests an increased likelihood of environmental dispersion of ARGs associated with anaerobically treated wastewater effluents and highlights the potential importance of persister cells in the dissemination of E. coli in the environment during reuse events of treated wastewater.IMPORTANCE This study examines the decay kinetics of a pathogenic and antibiotic resistant strain of Escherichia coli in microcosms simulating biological treatment units of aerobic and anaerobic sludge. The results of this study point at a significantly prolonged persistence of the E. coli and the associated antibiotic resistance gene in the anaerobic sludge. However, horizontal transfer of the plasmid encoding the antibiotic resistance gene was detected in the aerobic sludge by a cultivation method. A subpopulation of persister E. coli cells was also detected in the aerobic sludge. The findings of this study suggest potential areas of concern arising from pathogenic and antibiotic-resistant E. coli during both anaerobic and aerobic sludge treatment processes.

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“…(2009) Benzyl alcohol (54–108 mg/L) Pseudomonas putida (good survival at laboratory scale) pWWO (IncP-9 TOL plasmid, self-transmissible) Yes Yes but only at laboratory scale Pilot and laboratory scale sequencing batch biofilm reactors Venkata Mohan et al. (2009) 2,4-Dichlorophenoxyacetic acid (8–385 mg/L sole carbon source and with mixed substrates) Pseudomonas putida (poor survival, disappearance after 30 h) pJP4 (IncP-1 beta self-transmissible) Yes Yes Aerobic sludge granules in sequence batch reactors and microcosms ( Quan et al., 2010 ; Ma et al., 2012 ) 2,4-Dichlorophenoxyactic acid Escherichia coli HB101 or Pseudomonas putida KT2440 pJP4 (IncP-1β self-transmissible) Yes Unknown Filter matings with activated sludge microorganisms Tsutsui et al. (2010) 2,4-Dichlorophenoxyacetic acid (14–500 mg/L sole carbon source and with mix substrates) Pseudomonas putida SM1443 (poor survival, disappearance after 8 days) pJP4 (IncP-1 beta self-transmissible) Yes Yes but biodegradation was reduced when other carbon sources were present Aerobic sludge granules in sequence batch reactors Quan et al.…”

Section: Transfer Of Catabolic Genes As a Bioaugmentation Strategy Inmentioning

confidence: 99%

“…In more recent studies, Comamonas testosteroni , Escherichia coli , and Rhodococcus sp. were also applied ( Bathe et al., 2009 ; Tsutsui et al., 2010 ; Ren et al., 2018 ). Different MGEs were used, mainly plasmids.…”

Section: Transfer Of Catabolic Genes As a Bioaugmentation Strategy Inmentioning

confidence: 99%

The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment

2020

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Wastewater treatment plants (WWTPs) are crucial for producing clean effluents from polluting sources such as hospitals, industries, and municipalities. In recent decades, many new organic compounds have ended up in surface waters in concentrations that, while very low, cause (chronic) toxicity to countless organisms. These organic micropollutants (OMPs) are usually quite recalcitrant and not sufficiently removed during wastewater treatment. Microbial degradation plays a pivotal role in OMP conversion. Microorganisms can adapt their metabolism to the use of novel molecules via mutations and rearrangements of existing genes in new clusters. Many catabolic genes have been found adjacent to mobile genetic elements (MGEs), which provide a stable scaffold to host new catabolic pathways and spread these genes in the microbial community. These mobile systems could be engineered to enhance OMP degradation in WWTPs, and this review aims to summarize and better understand the role that MGEs might play in the degradation and wastewater treatment process. Available data about the presence of catabolic MGEs in WWTPs are reviewed, and current methods used to identify and measure MGEs in environmental samples are critically evaluated. Finally, examples of how these MGEs could be used to improve micropollutant degradation in WWTPs are outlined. In the near future, advances in the use of MGEs will hopefully enable us to apply selective augmentation strategies to improve OMP conversion in WWTPs.

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Transfer of plasmid pJP4 from Escherichia coli and Pseudomonas putida to bacteria in activated sludge developed under different sludge retention times

Cited by 21 publications

References 26 publications

Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone

Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone

Fate and Persistence of a Pathogenic NDM-1-Positive Escherichia coli Strain in Anaerobic and Aerobic Sludge Microcosms

The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment

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