Transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli

Gibson, Evelyn M.; Chace, Nina M.; London, S B; London, Jack

doi:10.1128/jb.137.1.614-619.1979

Cited by 100 publications

(36 citation statements)

References 16 publications

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“…We found that in small-scale preparations, PAM/~ 1 plasmid DNA was obtained at very low and irreproducible yields, as previously mentioned by West and Warner [ 181 for L. planturum PAM/~ 1 transconjugants. A similar result was reported when pAMp 1 was transferred to L. casei [19]. It was suggested that the plasmid had become integrated into the recipient chromosome or that a deletion had occurred upon transfer.…”

Section: Plasmid Dna Isolation and Analysissupporting

confidence: 74%

Intergeneric and intrageneric conjugal transfer of plasmids pAMÎ²1, pIL205 and pIP501 inLactobacillus sake

Langella

Zagorec²,

Ehrlich

et al. 1996

FEMS Microbiology Letters

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Transfer of the broad host range conjugative plasmids pAM β 1, pIP501 and its derivative pIL205 into Lactobacillus sake was studied. Frequencies up to 5 × 10−6 transconjugants per recipient cell were found from Lactococcus lactis strain IL1403 to various strains of L. sake. The transfer takes place by a conjugation‐like process on solid surface agar. Intrageneric transfer between L. sake strains was also observed, although at lower frequencies (10−7 transconjugants per recipient cell). The ability to transfer these plasmids into L. sake strains which are either refractory to transformation or poorly transformable provides a method of genetic transfer, thus opening the way for cloning strategies developed in other Gram‐positive bacteria.

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Section: Plasmid Dna Isolation and Analysissupporting

confidence: 74%

Intergeneric and intrageneric conjugal transfer of plasmids pAMÎ²1, pIL205 and pIP501 inLactobacillus sake

Langella

Zagorec²,

Ehrlich

et al. 1996

FEMS Microbiology Letters

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“…The introduction of broad host range transmissible plasmids was one of the earliest approaches used to establish basic genetic systems in the lactic acid bacteria. There are many reports of the conjugal transfer of the enterocoocal plasmid pAM/H into different strains, species and genera [64][65][66][67][68][69][70] although in some cases better results were obtained when alternative plasmids were used as in the transfer of piP501 in Pediococcus [71]. In matings between L, iactis and Bacillus subtilis a specific 10.5-kb deletion of pAMOl was observed and this prevented subsequent conjugal transfer [72].…”

Section: Heterologous Broad Host Range Transmissible Plasmidsmentioning

confidence: 99%

In vivo genetic systems in lactic acid bacteria

Gasson

1990

FEMS Microbiology Letters

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SUMMARYA review of in vivo genetic systems covers the key features of transduction and conjugation but emphasises the intramolecular and intermolecular DNA interactions that are often associated with these processes. As well as the transfer of many lactose plasmids, conjugal transfer of nisin genes and the use of conjugation to construct bacteriophage-resistant dairy starter cultures are discussed. The discovery and characterization of insertion sequences in Lactobacillus and Lactococcus and the exploitation of heterologous conjugation and transposition systems in the lactic acid bacteria are described.

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“…Several plasmids of this latter group can transfer to a wide range of hosts and confer resistance to erythromycin (Em'). An example of one such plasmid is pAM01 (4), which is transmissible to at least 10 streptococcal species (6,8,13,18,25), Staphylococcus aureus (6), Bacillus subtilis (16), and Lactobacillus casei (10).…”

mentioning

confidence: 99%

Streptococcal R plasmid pIP501: endonuclease site map, resistance determinant location, and construction of novel derivatives

Evans¹,

Macrina²

1983

J Bacteriol

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The streptococcal resistance plasmid pIP501 (30 kilobase pairs [kb]) encodes resistance to chloramphenicol (Cmr) and erythromycin (Emr) and is capable of conjugative transfer among numerous streptococcal species. By using a streptococcal host-vector recombinant DNA system, the Cmr and Emr determinants of pIP501 were localized to 6.3-kb HindlIl and 2.1-kb HindIII-AvaI fragments, respectively. pIP501 was lost at a frequency of 22% in Streptococcus sanguis cells grown at 42°C but was stable in cells grown at 37°C (<1% frequency of loss). Sequences from a cryptic multicopy plasmid, pVA380-1, were substituted for the pIP501 Emr determinant in vitro, and the resulting recombinant plasmid, designated pVA797, was recoverd in transformed S. sanguis cells. The replication of pVA797 was governed by the pVA380-1 sequences based on temperature-stable replication and incompatibility with pVA380-1-derived replicons. The self-ligation of partially cleaved HindIII pIP501 DNA fragments allowed the localization of a pIP501 region involved in autonomous plasmid replication. A small pIP501 derivative (pVA798) obtained from this experiment had a greatly increased copy number but was unstably inherited. Our data indicate that the sequences encoding the resistance determinants and some of the plasmid replication machinery are relatively clustered on the pIP501 molecule. The properties of pVA797 and pVA798 indicate that these molecules will enhance current streptococcal genetic systems from the standpoint of conjugative mobilization (pVA797) and gene amplification (pVA798).

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Transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli

Cited by 100 publications

References 16 publications

Intergeneric and intrageneric conjugal transfer of plasmids pAMÎ²1, pIL205 and pIP501 inLactobacillus sake

Intergeneric and intrageneric conjugal transfer of plasmids pAMÎ²1, pIL205 and pIP501 inLactobacillus sake

In vivo genetic systems in lactic acid bacteria

Streptococcal R plasmid pIP501: endonuclease site map, resistance determinant location, and construction of novel derivatives

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