Independent groups of rats underwent various pretraining vrocedures involving key light illumination and presentation of food before introduction of instrumental conditioning in which food reinforcement was immediately contingent upon pressing the illuminated key. Key illumination followed immediately by food presentation produced facilitation of subsequent acquisition of .instrumental reslJonding whiCh increased with 0, 10, 50, and 200 pretraining trials; the facilitative effect of 1,000 pairings of key illumination and food was no greater than that of 200 pairings. The facilitative effect of key illumination and food presentation upon subsequent instrumental learning was the same regardless of whether food presentations in pretraining immediately accompanined illumination offset or were delayed for 8 sec. Food presentations alone on 10 , 50, or 200 pretraining trials facilitated subsequellt instrumentallear,ling less than equivalent numbers of presentations of illumination and food, but 200 presentatio ns of fvod alone facilitated subsequent instrumental learning more than 0 pretraining trials.There have been several studies of the effects of response-independent palflngs of a stimulus and reinforcer upon subsequent appetitive instrumental discrimination learning. In a typical experiment, Bower and Grusec (1964) trained rats to press a lever for water. The lever then was removed and two tones were presented alternately. Water was periodically delivered during one tone presentation and not the other. The lever was then reintroduced, and discriminative instrumental training with the successively presented stimuli began. For some animals, response-contingent water reinforcement was delivered during presentation of the tone previously associated with water , and for others the discrimination was reversed . The former group developed appropriate discriminative responding more rapidly than did the group for which the stimulus relations were reversed . Mellgren and Ost (1969) confirmed this finding under similar conditions, and, by employing a control group which received water presentations during both stimuli before discriminative instrumental training, showed that the effect was due both to facilitation of performance in the group which experienced consistent stimulus-reinforcer relations and interference in the group in which the relations were reversed.