Transfer of Nitrogen Fixation (<i>nif</i>) Genes to Non‐diazotrophic Hosts

Li, Qin; Chen, Sanfeng

doi:10.1002/cbic.201900784

Cited by 31 publications

(17 citation statements)

References 70 publications

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“…However, N 2 -fixation poses a uniquely formidable challenge for high efficiency electromicrobial production. Over the past decade, several groups have incorporated genes for N 2 -fixation into E. coli and demonstrated functional N 2 -fixation ( Temme et al, 2012 ; Wang et al, 2013 ; Li et al, 2016 ; Yang et al, 2018 ; Li and Chen, 2020 ; Ryu et al, 2020 ). But, despite tantalizing possibilities ( MacKellar et al, 2016 ), all known nitrogenase enzymes are sensitive to O 2 .…”

Section: Discussionmentioning

confidence: 99%

Thermodynamic Constraints on Electromicrobial Protein Production

Wise

Marecos

Randolph

et al. 2022

Front. Bioeng. Biotechnol.

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Global consumption of protein is projected to double by the middle of the 21st century. However, protein production is one of the most energy intensive and environmentally damaging parts of the food supply system today. Electromicrobial production technologies that combine renewable electricity and CO2-fixing microbial metabolism could dramatically increase the energy efficiency of commodity chemical production. Here we present a molecular-scale model that sets an upper limit on the performance of any organism performing electromicrobial protein production. We show that engineered microbes that fix CO2 and N2 using reducing equivalents produced by H2-oxidation or extracellular electron uptake could produce amino acids with energy inputs as low as 64 MJ kg−1, approximately one order of magnitude higher than any previous estimate of the efficiency of electromicrobial protein production. This work provides a roadmap for development of engineered microbes that could significantly expand access to proteins produced with a low environmental footprint.

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Section: Discussionmentioning

confidence: 99%

Thermodynamic Constraints on Electromicrobial Protein Production

Wise

Marecos

Randolph

et al. 2022

Front. Bioeng. Biotechnol.

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“…However, N2-fixation poses a uniquely formidable challenge for high efficiency electromicrobial production. Over the past decade, several groups have incorporated genes for N2-fixation into E. coli and demonstrated functional N2-fixation [55][56][57][58][59][60] . But, despite tantalizing possibilities 61 , all known nitrogenase enzymes are sensitive to O2.…”

Section: Discussionmentioning

confidence: 99%

Thermodynamic Constraints on Electromicrobial Protein Production

Wise

Marecos

Randolph

et al. 2021

Preprint

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Global consumption of protein is projected to double by the middle of the 21st century1. However, protein production is one of the most energy intensive and environmentally damaging parts of the food supply system today. Electromicrobial production technologies that combine renewable electricity and CO2-fixing microbial metabolism could dramatically increase the energy efficiency of commodity chemical production2–5. Here we present a molecular-scale model that sets an upper limit on the performance of any organism performing electromicrobial protein production. We show that engineered microbes that fix CO2 and N2 using reducing equivalents produced by H2-oxidation or extracellular electron uptake could produce amino acids with energy inputs as low as 64 MJ kg-1. This work provides a roadmap for development of engineered microbes that could significantly expand access to proteins produced with a low environmental footprint.

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“…Thus, with the finding that the temperature in the mitochondria can reach 50 • C, the expression of nitrogenase subunits from thermophilic bacteria should be one of the approaches of future interest [77]. Additionally, with further research, it might be possible in the future to construct functional nitrogenases in plant cells and create a practical crop that can fix nitrogen by itself [78]. Recently, researchers have succeeded in heterologously expressing a nitrogenase that is active within the chloroplast of the cyanobacterium Synechococcus elongatus PCC7942, and have also succeeded in creating algae that can fix nitrogen using energy from photosynthesis [79].…”

Section: Ammonia Production Using Nitrogenasementioning

confidence: 99%

Sustainable Biological Ammonia Production towards a Carbon-Free Society

2021

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A sustainable society was proposed more than 50 years ago. However, it is yet to be realised. For example, the production of ammonia, an important chemical widely used in the agriculture, steel, chemical, textile, and pharmaceutical industries, still depends on fossil fuels. Recently, biological approaches to achieve sustainable ammonia production have been gaining attention. Moreover, unlike chemical methods, biological approaches have a lesser environmental impact because ammonia can be produced under mild conditions of normal temperature and pressure. Therefore, in previous studies, nitrogen fixation by nitrogenase, including enzymatic ammonia production using food waste, has been attempted. Additionally, the production of crops using nitrogen-fixing bacteria has been implemented in the industry as one of the most promising approaches to achieving a sustainable ammonia economy. Thus, in this review, we described previous studies on biological ammonia production and showed the prospects for realising a sustainable society.

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Transfer of Nitrogen Fixation (nif) Genes to Non‐diazotrophic Hosts

Cited by 31 publications

References 70 publications

Thermodynamic Constraints on Electromicrobial Protein Production

Thermodynamic Constraints on Electromicrobial Protein Production

Thermodynamic Constraints on Electromicrobial Protein Production

Sustainable Biological Ammonia Production towards a Carbon-Free Society

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