Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae

Ferenczy, L.; Maráz, Anna

doi:10.1038/268524a0

Cited by 80 publications

(34 citation statements)

References 14 publications

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“…Protoplast fusion has been employed previously to restore respiratory competency to petite cells of S. cerevisiae (Maraz and Ferenczy 1980) and offers the possibility of transferring intact organelles (e.g. the mitochondrion) between cells (Ferenczy and Maraz 1977). The selection conditions employed here screened for the transfer of mitochondria in the construction of the cybrids, rather than the transfer of chromosomes, which is a far rarer event (Kavanagh and Whittaker 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Disruption of the mitochondrial cytochrome content of a cell has the potential to reduce its respiratory efficacy, while restoration of respiratory function following transfer of a mitochondrion by protoplast fusion has the capacity to restore, partially or completely, respiratory status (Ferenczy and Maraz 1977;Maraz and Ferenczy 1980 (Fig. 2).…”

Section: Determination Of Respiratory Status Of C Albicans Strainsmentioning

confidence: 99%

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Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B

Geraghty

Kavanagh

2003

Arch Microbiol

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A respiratory-deficient mutant of Candida albicans MEN was generated by culturing cells in medium supplemented with ethidium bromide at 37°C for 5 days. The respiratory-deficient mutant (C. albicans MMU11) was incapable of growth on glycerol, had a reduced oxygen uptake rate and demonstrated an altered mitochondrial cytochrome profile. Respiratory-competent cybrids were formed by mitochondrial transfer following fusion of protoplasts with those of C. albicans ATCC 44990. Mutant MMU11 possessed lower levels of ergosterol than the parental isolates and the cybrids, and demonstrated a small but statistically significant increase in tolerance to amphotericin B. The results demonstrated that disruption of mitochondrial function in C. albicans increases the tolerance to amphotericin B, possibly mediated by a reduction in cellular ergosterol content.

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Section: Discussionmentioning

confidence: 99%

Section: Determination Of Respiratory Status Of C Albicans Strainsmentioning

confidence: 99%

Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B

Geraghty

Kavanagh

2003

Arch Microbiol

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“…patterns of non-saponifiable sterol extracts of cells, and ultraviolet spectrophotometry of ergosterol content have been described previously (Pesti et Protoplast fusion and regeneration. The methods described by Ferenczy & Maraz (1977) for Saccharomyces cerevisiae were used.…”

Section: E T H O D Smentioning

confidence: 99%

Protoplast Fusion Hybrids of Candida albicans Sterol Mutants Differing in Nystatin Resistance

Pesti¹,

Ferenczy²

1982

Microbiology

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Intraspecific protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out in various pairings between auxotrophic nystatin-sensitive, ergosterol-producing strains of Candida albicans and their nystatin-resistant, ergosterol-less mutants of different origins. Nutritionally-complemented stable heterozygous diploid hybrids were obtained, which proved to be sensitive, semi-resistant or resistant to nystatin as a consequence of complementation or non-complementation for ergosterol biosynthesis. Dominant mutation control of the resistance was not found.

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“…The efficient binding of plasmid DNA to protoplasts is apparently a prerequisite for efficient transformation as both the binding and transformation required Ca2+ and were inhibited when foreign sperm DNA was present ( fig.2). On the other hand, transformation was dependent, and affected in a similar manner, by those factors which determine fusion of protoplasts ( fig.2): both transformation [l] and fusion [6,7] are dependent on the presence of PEG; and both transformation ( fig.2) and fusion [8] are more efficient at alkaline pH. When protoplasts were preincubated with plasmid for 20 min and then DNase (+ MgC12) was added and further incubated for 30 min, no transformation occurred.…”

Section: Resultsmentioning

confidence: 99%

Interaction of plasmid DNA with yeast protoplasts and a mechanism of genetic transformation

Brzobohatý

Kováč

1985

FEBS Letters

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DNA of plasmid pBB 29 is bound to protoplasts of Saccharomyces cerevisiae by a time-dependent, energyindependent process requiring Ca 2+. It is not degraded during binding but most of the bound DNA can be degraded by exogenously added DNase. Foreign DNA competes for the binding. Genetic transformation of protoplasts depends on the same conditions as does the binding of DNA but, in addition, it also requires conditions allowing fusion of the protoplasts. Transformation may consist of firm adsorption of plasmid DNA to the protoplast surface, mediated by CaZ+, and of internalization of the DNA entrapped between two aggregated protoplasts as a suit of their fusion. CelI fusionPlasmid DNA

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Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae

Cited by 80 publications

References 14 publications

Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B

Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B

Protoplast Fusion Hybrids of Candida albicans Sterol Mutants Differing in Nystatin Resistance

Interaction of plasmid DNA with yeast protoplasts and a mechanism of genetic transformation

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