Transfer of Methanogenium bourgense, Methanogenium marisnigri, Methanogenium olentangyi, and Methanogenium thermophilicum to the Genus Methanoculleus gen. nov., Emendation of Methanoculleus marisnigri and Methanogenium, and Description of New Strains of Methanoculleus bourgense and Methanoculleus marisnigri

Maestrojuan, Gloria M.; Boone, David R.; Xun, Luying; Mah, Robert A.; Zhang, Lanfang

doi:10.1099/00207713-40-2-117

Cited by 99 publications

(38 citation statements)

References 22 publications

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“…Samples were collected and were immediately serially diluted in MS enrichment medium (similar to MS medium but with decreased concentrations of organic compounds [22]) supplemented with 100 mM methanol as the substrate. Cultures at the highest dilution which produced methane were selected and maintained by periodic transfers to fresh medium.…”

Section: Lycmentioning

confidence: 99%

Taxonomy and Halotolerance of Mesophilic Methanosarcina Strains, Assignment of Strains to Species, and Synonymy of Methanosarcina mazei and Methanosarcina frisia

Maestrojuan¹,

Boone²,

Mah

et al. 1992

International Journal of Systematic Bacteriology

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We examined 22 previously described and newly isolated Methanosarcina strains by performing denaturing gel electrophoresis of whole-cell proteins and assigned these strains to previously described species. Methanosarcina mazei S-6T (T = type strain) and Methanosarcinafi.isia C 16T were very similar in terms of the electrophoresis patterns of their proteins and in their DNA sequences (the results of reassociation experiments indicated that there was 77% sequence similarity). Thus, M. fish is a junior subjective synonym of M. mazei, and strain C 16 is a reference strain of M. mazei. M. muzei C 16 was similar to M. mazei in other characteristics that have not been reported previously, including the ability to catabolize acetate and a lack of halophily. All of the Methanosarcina strains examined, including the marine strains M. mazei C 16 (= M. jiisia C 16T) and Methunosarcina acetivorans C2AT, were slightly halotolerant (rather than halophilic, as originally described). Methunosarcina sp. strain FR-1, which has gas vesicles, was more similar to Methanosarcina barken' MST than to Methanosarcina vacuolata Z-761T in both its protein patterns and its DNA sequence (80% similarity to M. barken' MST and 38% similarity to M. vacuolata Z-761T). Thus, the presence of gas vesicles is not an adequate taxonomic characteristic for assigning Methunosarcina strains to M. vacuolata.The five commonly recognized mesophilic Methanosarcina species (Methanosarcina barkri, Methanosarcina mazei, Methanosarcina vacuolata , Methanosarcina fnsia , and Methanosarcina acetivorans) are found in marine and freshwater environments (24,33,34). The first three of these species are considered to be freshwater species, and their type strains were isolated from freshwater environments. However, early strains of M. barkri were isolated from marine or brackish mud (36,42), and the current type strains of M. barkeri and M. mazei thrive at marine salinities (40). It has been postulated that Methanosarcina species evolved in marine habitats and adapted to freshwater environments by developing a heteropolysaccharide matrix or capsule (41). When Methanosarcina strains are grown in the presence of marine concentrations of Na+ and Mg2+, they have a protein cell wall, are sensitive to lysis by detergents, and occur as individual cells (35,40,46). When these organisms are grown in the presence of low concentrations of these cations, a heteropolysaccharide matrix is often formed, and cells may occur as aggregates, "cysts," or lamina (27,40,46).The other two mesophilic Methanosarcina species, M. fnsia and M. acetivorans, are considered to be marine species because all of the known strains (M. fnsia C 16T [T = type strain] and M. acetivorans C2AT and C2E) were isolated from marine sediments (4, 39). The cells of these strains are sensitive to lysis by detergent, and their cell walls are protein (4, 39).In this paper we describe some of the physiological characteristics, especially halotolerance, of some Methanosarcina strains, the results of a phylogenetic analysis...

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Section: Lycmentioning

confidence: 99%

Taxonomy and Halotolerance of Mesophilic Methanosarcina Strains, Assignment of Strains to Species, and Synonymy of Methanosarcina mazei and Methanosarcina frisia

Maestrojuan¹,

Boone²,

Mah

et al. 1992

International Journal of Systematic Bacteriology

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“…In addition to phylogenetic distance, this separation is supported by two phenotypic distinctions: strain Nankai-1 did not require peptones for growth, and its maximum temperature was much higher (54°C). M. marisnigri JR1 grows well at 45°C but does not grow at 50°C (19). The low sequence similarity of the DNAs of strain Nankai-1 and M. marisnigri JR1 and the important phenotypic differences meet the recommendations for placement of an organism into a new species (35).…”

Section: Discussionmentioning

confidence: 88%

Isolation of a Methanogen from Deep Marine Sediments That Contain Methane Hydrates, and Description of Methanoculleus submarinus sp. nov

Mikucki

Liu

Delwiche

et al. 2003

Appl Environ Microbiol

150

100

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We isolated a methanogen from deep in the sediments of the Nankai Trough off the eastern coast of Japan. At the sampling site, the water was 950 m deep and the sediment core was collected at 247 m below the sediment surface. The isolated methanogen was named Nankai-1. Cells of Nankai-1 were nonmotile and highly irregular coccoids (average diameter, 0.8 to 2 m) and grew with hydrogen or formate as a catabolic substrate. Cells required acetate as a carbon source. Yeast extract and peptones were not required but increased the growth rate. The cells were mesophilic, growing most rapidly at 45°C (no growth at <10°C or >55°C). Cells grew with a maximum specific growth rate of 2.43 day ؊1 at 45°C. Cells grew at pH values between 5.0 and 8.7 but did not grow at pH 4.7 or 9.0. Strain Nankai-1 grew in a wide range of salinities, from 0.1 to 1.5 M Na ؉ . The described phenotypic characteristics of this novel isolate were consistent with the in situ environment of the Nankai Trough. This is the first report of a methanogenic isolate from methane hydrate-bearing sediments. Phylogenetic analysis of its 16S rRNA gene sequence indicated that it is most closely related to Methanoculleus marisnigri (99.1% sequence similarity), but DNA hybridization experiments indicated a DNA sequence similarity of only 49%. Strain Nankai-1 was also found to be phenotypically similar to M. marisnigri, but two major phenotypic differences were found: strain Nankai-1 does not require peptones, and it grows fastest at a much higher temperature. We propose a new species, Methanoculleus submarinus, with strain Nankai-1 as the type strain.Marine methane hydrates are an ice-like material in which methane molecules are trapped within cages of the crystalline lattice of water molecules. Methane hydrates form at temperatures of up to about 15 or 16°C when the partial pressure of methane is very high. This occurs in many deep marine sediments, where temperatures are low and the hydrostatic pressure of the water keeps methane in solution at high partial pressures (29). Gas hydrates are globally distributed along coastal margins, trapping enormous volumes of methane, estimated at about twice the amount of all other known fossil fuel reserves (1). Methane hydrates have been estimated to contain roughly 4,000 times today's atmospheric content of methane (4). With most of the methane trapped in known hydrate formations being of biogenic origin, this represents a significant new source of natural gas from biological methanogenesis.Natural gas hydrates typically occur along coastal margins where organic matter accumulates. Microbial numbers are high in these zones: 1.5 ϫ 10 9 cells g Ϫ1 of hydrate-bearing sediments and 1.0 ϫ 10 6 cells ml Ϫ1 within the hydrates themselves (17). Metabolic studies of these sediments, based on incubations with stable isotopes of substrate and subsequent measurement of the isotope-containing product, have demonstrated methanogenesis, sulfate reduction, and methane oxidation (8). Phylogenetic analysis of DNA extracted from marine sedim...

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“…All of the archaeal clones were closely related to the following three strains of Methanoculleus genus: i) Methanoculleus sp. SLH121 isolated from the extreme environment of Haakon Mosby mud volcano (GenBank accession number: KC893306), ii) Methanoculleus chikugoensis isolated from paddy field soil in Japan (Dianou et al, 2001;Oren, 2014) and iii) Methanoculleus marisnigri isolated from Black Sea sediment (Romesser et al, 1979;Maestrojuan et al, 1990;Oren, 2014). Members of the genus Methanoculleus are among the dominant methanogenic archaea found in biogas-producing reactor systems fed with renewable primary products (e.g.…”

Section: Discussionmentioning

confidence: 99%

Preliminary assessment of methanogenic microbial communities in marine caves of Zakynthos Island (Ionian Sea, Greece)

et al. 2018

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Mediterranean marine caves remain largely unexplored, while particularly limited information is available about microbial life in these unique environments. This study is a preliminary assessment of the composition of the active anaerobic microbial community colonizing the walls of newly explored systems of underwater caves and small cavities in Zakynthos Island. The interior of these caves is densely coated with egg-shaped, foam-shaped and filamentous biological structures that are characterised by a strong odour of hydrogen sulphide gas. A total of twelve structures scrapped from cave rocks were subjected to anaerobic cultivation for up to 208 days. Strong to moderate methanogenesis was observed in two different types of egg-shaped structures and one foamlike structure. Interestingly, this was observed in experiments that were performed at room temperature (i.e. 25 o C), which is substantially lower than those typically considered optimum for methane production (e.g. 35 o C). An analysis of the 16S rRNA genes revealed a clear dominance of archaea and bacteria closely related to known methane producers and sulphate reducers, including members of the families Methanomicrobiaceae, Desulfobulbaceae, Desulfobacteraceae, Desulfuromonaceae, Campylobacteraceae, Marinifilaceae, Clostridiaceae, Incertae Sedis -Family I & II. These results show that Mediterranean marine caves can host members of archaea and bacteria with potential biotechnological interest that deserves further investigation.

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Cited by 99 publications

References 22 publications

Taxonomy and Halotolerance of Mesophilic Methanosarcina Strains, Assignment of Strains to Species, and Synonymy of Methanosarcina mazei and Methanosarcina frisia

Taxonomy and Halotolerance of Mesophilic Methanosarcina Strains, Assignment of Strains to Species, and Synonymy of Methanosarcina mazei and Methanosarcina frisia

Isolation of a Methanogen from Deep Marine Sediments That Contain Methane Hydrates, and Description of Methanoculleus submarinus sp. nov

Preliminary assessment of methanogenic microbial communities in marine caves of Zakynthos Island (Ionian Sea, Greece)

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