Transfer of a two-tiered keratinocyte assay: IL-18 production by NCTC2544 to determine the skin sensitizing capacity and epidermal equivalent assay to determine sensitizer potency

Teunis, Marc; Corsini, Emanuela; Smits, Mieke; Madsen, Charlotte Bernhard; Eltze, Tobias; Ezendam, Janine; Galbiati, Valentina; Gremmer, Eric R.; Krul, Cyrille; Landin, Annette; Landsiedel, Robert; Pieters, Raymond; Rasmussen, Tina Frid; Reinders, Judith; Roggen, Erwin L.; Spiekstra, Sander W.; Gibbs, Susan

doi:10.1016/j.tiv.2012.06.004

Cited by 42 publications

(36 citation statements)

References 29 publications

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“…For a full description of the technology transfer and standard operating procedure (SOP) for the EE potency assay see supplementary materials in Teunis et al (2013). Following the SOP, any chemical that is soluble in DMSO or a mixture (4:1) of acetone:olive oil (AOO) can be tested.…”

Section: Methods Outlinementioning

confidence: 99%

“…After having assessed the transferability of the ee potency assay (Teunis et al, 2013), the ring trial reported here, involving four European laboratories: VUMC (lead laboratory), HU, DiSFeB and BASF, was started. Thirteen coded well-known sensitizing chemicals were used (Tab.…”

Section: Methods Outlinementioning

confidence: 99%

“…The positive control chemical was resorcinol (60 mg/ml (545 mM) in 1% DMSO). This concentration was selected from past experience by the VUMC lead laboratory (Teunis et al, 2013;dos Santos et al, 2011). For each experiment, unexposed, vehicle-exposed, and positive controls were tested in duplicate and the test chemical concentrations in single fold.…”

Section: Epidermal Equivalent Skin Modelsmaintenance and Chemical Expmentioning

confidence: 99%

“…Previously, the transfer phase of the EE potency assay international ring trial has been reported (Teunis et al, 2013). The transferability of the standard operating procedure (SOP) from the lead laboratory (VUMC) to 3 other European laboratories (University of Applied Sciences Utrecht, The Netherlands (HU), University of Milan, Italy (DiSFeB) and BASF Chemical Company, Ludwigshafen, Germany (BASF)) was assessed using two training chemicals (DNCB and resorcinol).…”

Section: Introductionmentioning

confidence: 99%

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International ring trial of the epidermal equivalent sensitizer potency assay: reproducibility and predictive capacity

Teunis

2014

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Summary This study describes the international ring trial of the epidermal-equivalent (EE) sensitizer potency assay. This assay does not distinguish a sensitizer from a non-sensitizer, but may classify known skin sensitizers according to their potency. It assesses the chemical concentration resulting in 50% cytotoxicity (EE-EC50)or the 2-fold increase in Abbreviations: AOO, acetone:olive oil (4:1); BD, broad dose; EE, epidermal equivalent; EC 50 , chemical concentration in mg/ml that results in a decrease in cell viability to 50% compared to vehicle treated epidermal equivalents; DiSFeB, Dipartimento di Scienze Farmacologiche e Biomolecolari, Milan University, Milan; DMSO, 1% dimethylsulfoxide; DSA 05 , chemical dose per skin area in µg/cm 2 leading to a sensitization incidence of 5% in the human tested population; FD, fine dose; GPMT, Guinea Pig Maximization Test; HU, University of Applied Sciences, Utrecht; HRIPT, Human Repeat Insult Patch Test; IL-1α 2x , Chemical concentration in mg/ml resulting in a 2-fold increase in IL-1α release into culture supernatant of EE compared to supernatant of vehicle-exposed EE; LLNA, Local Lymph Node Assay; NOEL, human threshold level (no observed effect level) expressed in µg/cm 2 ; OECD, Organisation for Economic Co-operation and Development; SOP, standard operating procedure; VUMC, VU University Medical Center, Amsterdam

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Section: Methods Outlinementioning

confidence: 99%

Section: Methods Outlinementioning

confidence: 99%

Section: Epidermal Equivalent Skin Modelsmaintenance and Chemical Expmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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International ring trial of the epidermal equivalent sensitizer potency assay: reproducibility and predictive capacity

Teunis

2014

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“…the analysis of 33 known chemicals led to protocol optimization, and most critical parameters were highlighted during a prevalidation exercise funded by the Dutch governmental funding agency ZonMW (Teunis et al, 2012).…”

Section: The Nctc2544 Assay (By Emanuela Corsini)mentioning

confidence: 99%

Advanced tests for skin and respiratory sensitization assessment

Rovida

Martin

Vivier

et al. 2013

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Denmark, aided by the vice-coordinator Hans-Ulrich Weltzien from the Max-Planck Institute for Immunobiology and epigenetics, Freiburg Germany. the ultimate goal of the Sens-it-iv project was the development of a set of in vitro methods for the assessment of the skin and respiratory sensitization potential of chemicals and proteins. the level of development was intended to be at the point to enter the pre-validation phase. After 66 months of work, it could be concluded that the goal was largely accomplished. Several advanced methods were evaluated extensively, and for some of them a detailed standard operating procedure (SOP) was established for pre-validation purposes. Other, less advanced methods contributed to our understanding of the mechanisms driving sensitization as well.Several opportunities were employed to assure dissemination of results. During the course of the project, 44 newsletters were released, presenting the main achievements of the project. Fly-1 Introduction

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The NCTC 2544 IL‐18 Assay for the In Vitro Identification of Contact Allergens

Galbiati

Corsini

2012

CP Toxicology

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Assessment of allergenic potency of low-molecular-weight compounds is generally performed using animal models, such as the murine Local Lymph Node Assay (LLNA) and the Guinea Pig Maximization Test (GMT). Progress in understanding the mechanism of skin sensitization, including effects on the production of cytokines by different cell types within the skin, provides the opportunity to develop in vitro tests as an alternative to in vivo sensitization testing. This unit will describe a method for differentiating contact allergens from low-molecular-weight respiratory allergens and irritants, based on the selective induction of intracellular interleukin 18 (IL-18) in the human keratinocyte cell line NCTC 2544. Similar results could also be obtained using primary human keratinocytes or other human keratinocyte cell lines, e.g., HaCaT, HPKII, etc. IL-18 was chosen because this cytokine has been demonstrated to favor Th-1-type immune responses by enhancing the secretion of pro-inflammatory mediators such as TNF-α, IL-8, and IFN-γ, and to play a key proximal role in the induction of allergic contact dermatitis.

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Transfer of a two-tiered keratinocyte assay: IL-18 production by NCTC2544 to determine the skin sensitizing capacity and epidermal equivalent assay to determine sensitizer potency

Cited by 42 publications

References 29 publications

International ring trial of the epidermal equivalent sensitizer potency assay: reproducibility and predictive capacity

International ring trial of the epidermal equivalent sensitizer potency assay: reproducibility and predictive capacity

Advanced tests for skin and respiratory sensitization assessment

The NCTC 2544 IL‐18 Assay for the In Vitro Identification of Contact Allergens

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