Transfection of Smac/DIABLO sensitizes drug-resistant tumor cells to TRAIL or paclitaxel-induced apoptosis in vitro

Mao, Hong Luan; Liu, Pei Shu; Zheng, Jing; Zhang, Pei hai; Zhou, Li; Xin, Gang; Liu, Chunxi

doi:10.1016/j.phrs.2007.09.010

Cited by 26 publications

(29 citation statements)

References 29 publications

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“…Thus, Smac downregulation may contribute to esophageal carcinogenesis. Reduced Smac expression has also been described in other types of cancer (11,38,(40)(41)(42). Importantly, our data revealed a significant association between Smac expression and chemotherapeutic response among the 31 ESCC patients who had undergone chemotherapy.…”

Section: Discussionsupporting

confidence: 77%

Role of Smac in Determining the Chemotherapeutic Response of Esophageal Squamous Cell Carcinoma

Zhou

Huang

et al. 2011

Clinical Cancer Research

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Purpose: Second mitochondria-derived activator of caspase (Smac) regulates chemotherapy-induced apoptosis. Smac mimetics have been tested in clinical trials as chemosensitizers. We determined the role of Smac in modulating the chemosensitivity of esophageal squamous cell carcinoma (ESCC).Experimental Design: Smac expression was evaluated in tissues from ESCC patients with differential chemotherapeutic responses. The effects of Smac knockdown and Smac mimetics on the chemosensitivity of ESCC cells and the molecular mechanisms by which Smac and Smac mimetics modulate chemosensitivity were determined. The therapeutic responses of ESCC cells with different Smac statuses were compared using xenograft models.Results: We found that Smac was significantly downregulated in most ESCC samples (36.8%, 25/68, P ¼ 0.001), and Smac expression differed significantly (P < 0.05) between chemosensitive and chemoresistant tumors. The associations of tested factors and their responses were examined using logistic regression analysis. In ESCC cells treated with cisplatin, a common chemotherapeutic drug, Smac and cytochrome c were released from mitochondria, and caspase-3 and caspase-9 were activated.

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Section: Discussionsupporting

confidence: 77%

Role of Smac in Determining the Chemotherapeutic Response of Esophageal Squamous Cell Carcinoma

Zhou

Huang

et al. 2011

Clinical Cancer Research

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“…It is a protein released from mitochondria in response to apoptotic stimuli. Smac/DIABLO antagonizes inhibitor of apoptosis proteins (IAPs) to relieve their inhibitory effects on caspases [27]. The obtained results demonstrated that 24 h treatment with osthole increased significantly Smac/DIABLO in the level of mRNA expression in all cell lines.…”

Section: Resultsmentioning

confidence: 99%

“…Quality and quantity of total RNA were assessed by spectrophotometer (NanoDrop 2000, USA) and samples were stored at −80°C until use. The primer sequences used for PCR were β -actin: 5′-TCATGAAGTGTGTGACGTGGACATC-3 (forward) and 5′-CAGGAGGAGCAATGATCTTGATCT-3′ (reverse); Bcl-2: 5′-ATCGCCCTGTGGATGACTGAG-3′ (forward) and 5′-GACCCAGGAGAAATCAAACAGAGG-3′ (reverse); Bax: 5′-GGACGAACTGGACAGTAACATCG-3′ (forward) and 5′-GCAAAGTAGAAAAGGGCGACAAC-3′ (reverse); and Smac/DIABLO: 5′-AGCTGGAAACCACTTGGATG-3′ (forward) and 5′-CCAGCTTGGTTTCTGCTTT-3′ (reverse) [27, 28]. The performances of all primer pairs were tested by primer concentration to determine the optimal reaction conditions.…”

Section: Methodsmentioning

confidence: 99%

Comparative Evaluation of Cytotoxic and Apoptogenic Effects of Several Coumarins on Human Cancer Cell Lines: Osthole Induces Apoptosis in p53-Deficient H1299 Cells

Shokoohinia

Hosseinzadeh

Alipour

et al. 2014

Advances in Pharmacological Sciences

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Natural products are excellent resources for finding lead structures for the development of chemotherapeutic agents. Coumarins are a class of natural compounds found in a variety of plants. In this study, we evaluated the cytotoxic potential of coumarins isolated from Prangos ferulacea (L.) Lindl. in PC3, SKNMC, and H1299 (p53 null) human carcinoma cell lines. Osthole proved to be an outstanding potent cytotoxic agent especially against PC3 cells. Isoimperatorin exhibited moderate inhibitory effect against SKNMC and PC3 cell lines. Oxypeucedanin and braylin did not display any cytotoxic activity. In the next set of experiments, the apoptotic potentials of osthole and isoimperatorin were investigated. Induction of apoptosis by isoimperatorin was accompanied by an increase in activation of caspase-3, -8, and -9 in SKNMC cells and caspase-3 and -9 in PC3 cells. Moreover, isoimperatorin induced apoptosis by upregulating Bax and Smac/DIABLO genes in PC3 and SKNMC cells. Osthole induced apoptosis by downregulating antiapoptotic Bcl-2 in only PC3 cells and upregulating the proapoptotic genes Bax and Smac/DIABLO in PC3, SKNMC, and H1299 cells. The effects of osthole on H1299 cells are important because the loss of p53 has been associated with poor clinical prognosis in cancer treatment.

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“…In this survey, strong XIAP staining was most prevalent in ovarian carcinoma effusions and less prevalent in mammary carcinoma effusions. XIAP expression increases with advancing stage in ovarian carcinoma (Mao et al, 2007). XIAP is also identified as part of a progression signature in prostate cancer using an immunoblot approach to characterize proteomic alterations in prostate tumors.…”

Section: Pathological Roles Of the Iaps In Cancermentioning

confidence: 99%

IAP-targeted therapies for cancer

et al. 2008

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Transfection of Smac/DIABLO sensitizes drug-resistant tumor cells to TRAIL or paclitaxel-induced apoptosis in vitro

Cited by 26 publications

References 29 publications

Role of Smac in Determining the Chemotherapeutic Response of Esophageal Squamous Cell Carcinoma

Role of Smac in Determining the Chemotherapeutic Response of Esophageal Squamous Cell Carcinoma

Comparative Evaluation of Cytotoxic and Apoptogenic Effects of Several Coumarins on Human Cancer Cell Lines: Osthole Induces Apoptosis in p53-Deficient H1299 Cells

IAP-targeted therapies for cancer

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