Transfection of oocytes and other types of ovarian cells in rabbits after direct injection into uterine arteries of adenoviruses and plasmid/liposomes

Laurema, Anniina; Heikkilä, Annaleena; Keski‐Nisula, Leea; Heikura, Tommi; Lehtolainen, Pauliina; Manninen, Hannu; Tuomisto, Tiina T.; Heinonen, Seppo; Ylä‐Herttuala, Seppo

doi:10.1038/sj.gt.3301918

Cited by 16 publications

(23 citation statements)

References 18 publications

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“…The results indicated that strong b-gal expressions could be detected in tumor tissues, and RT-PCR and Western blot analyses demonstrated high expression levels of HSV 1 -tk mRNA and protein in the ovarian tumors when they were administrated through the ovarian artery, and extremely low levels in the organs. These results were found to be similar to those reported by Laurema et al 29 Through artery administration, a high concentration could be obtained in the target organs of artery with a relative low dose of drug, 30 and we got herein the same results. GCV was administered i.p.…”

Section: Discussionsupporting

confidence: 91%

Enhanced efficiency and specificity of ovarian cancer gene therapy in rats with a novel nonviral gene delivery system (GE7) via intraovarian artery perfusion approach

et al. 2005

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Transfer of the herpes simplex virus type I-thymidine kinase gene, followed by the administration of ganciclovir (HSV 1 -tk/GCV) into ovarian cancer-derived cell line either in vitro or transplanted into nude mice has been shown to provide a potential strategy for the gene therapy of ovarian cancer. We investigated the antitumor effects of HSV 1 -tk/GCV strategy with a chemically induced rat ovarian cancer model and a tumor-selective gene delivery by a novel nonviral gene delivery system (GE7) through the ovarian artery and tail vein. We demonstrated the expression of a reporter gene, b-gal gene, as well as HSV 1 -tk gene in tumors and other organs, evaluated the overall antitumor effects after the GCV treatment and analyzed the tumor cell cycle phase distribution. Via the ovarian artery route, the expressions of b-gal and HSV 1 -tk in tumors were significantly stronger than those expressed in such organs as the hearts, livers, spleens, lungs and kidneys. However, no b-gal and HSV 1 -tk were detected in the tumor tissues when administrated via the tail vein, and little was found in other organs. The cell cycle analysis showed that the total S-phase of tumor cells in the test intra-arterial treatment group was considerably higher than that of the controls. The weight of the tumor tissues in the group treated by the intra-arterial route (4.0672.12 g) was much less than the group treated intravenously (18.2578.34 g) (Po.01). These findings indicated that the administration of GE7/HSV 1 -tk complex via the ovarian artery route could be a promising avenue of future human ovarian cancer treatment.

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Section: Discussionsupporting

confidence: 91%

Enhanced efficiency and specificity of ovarian cancer gene therapy in rats with a novel nonviral gene delivery system (GE7) via intraovarian artery perfusion approach

et al. 2005

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“…Arruda et al [39], who investigated the risk of inadvertent germ-line transmission by administration of an AAV type 2 vector to adult mice, rats, rabbits and dogs, detected no vector DNA in the semen of the animals. In contrast, gene transfer into oocytes and other types of ovarian cells in rabbits by direct injection of adenoviruses and plasmidliposome mixtures into uterine arteries has been reported [44], as well as integration of a marker gene into germ cells with transmission to the next generation following prenatal intraperitoneal plasmid injection in rats [33]. Furthermore, transgene expression in spermatogonia-like cells following AAV type 2 administration to the myocard of rats has been observed [29], and vector DNA was found in the semen of patients enrolled in a clinical phase I study of liver-directed AAV type 2 mediated hfIX gene transfer, where positive PCR signals persisted for several weeks [28].…”

Section: Analysis Of Germ Cellsmentioning

confidence: 89%

No evidence for germ‐line transmission following prenatal and early postnatal AAV‐mediated gene delivery

Jakob

Mühle

Park

et al. 2005

The Journal of Gene Medicine

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“…LMD enables the isolation of relatively pure cell populations from well characterized tissue samples. 7,12 It is important to note that these macrophage-rich areas also contained some T cells (Ͻ1% to 2%), and it is possible that some genes are derived from T cells. However, it can still be concluded that macrophage-rich areas were successfully isolated since (1) several genes typical for macrophages were identified, (2) T-cell content was low, (3) expression changes in endothelial cell and T-cell-specific genes were not significant, and (4) similar changes were found in vitro in PMA-stimulated THP-1 macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…Macrophage-rich shoulder areas, adjacent lesion cap areas containing no inflammatory cells, and areas of macroscopically normal DIT were dissected (Leica DM LMD system, Leica) from the same individuals. 12 …”

Section: Histology and Laser Microdissectionmentioning

confidence: 99%

Gene Expression in Macrophage-Rich Inflammatory Cell Infiltrates in Human Atherosclerotic Lesions as Studied by Laser Microdissection and DNA Array

Tuomisto

Korkeela

Rutanen

et al. 2003

ATVB

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Objective-Inflammatory cells play an important role in atherogenesis. However, more information is needed about their gene expression profiles in human lesions. Methods and Results-We used laser microdissection (LMD) to isolate macrophage-rich shoulder areas from human lesions. Gene expression profiles in isolated cells were analyzed by cDNA array and compared with expression patterns in normal intima and THP-1 macrophages. Upregulation of 72 genes was detected with LMD and included 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, interferon regulatory factor-5 (IRF-5), colony stimulating factor (CSF) receptors, CD11a/CD18 integrins, interleukin receptors, CD43, calmodulin, nitric oxide synthase (NOS), and extracellular superoxide dismutase (SOD). Several of these changes were also present in PMA-stimulated THP-1 macrophages in vitro. On the other hand, expression of several genes, such as VEGF, tissue factor pathway inhibitor 2, and apolipoproteins C-I and C-II, decreased. Conclusions-Overexpression of HMG-CoA reductase in macrophage-rich lesion areas may explain some beneficial effects of statins, which can also modulate increased expression of CD11a/CD18 and CD43 found in microdissected cells. We also found increased expression of CSF receptors, IRF-5, and interleukin receptors, which could become useful therapeutic targets for the treatment of atherosclerotic diseases.

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Transfection of oocytes and other types of ovarian cells in rabbits after direct injection into uterine arteries of adenoviruses and plasmid/liposomes

Cited by 16 publications

References 18 publications

Enhanced efficiency and specificity of ovarian cancer gene therapy in rats with a novel nonviral gene delivery system (GE7) via intraovarian artery perfusion approach

Enhanced efficiency and specificity of ovarian cancer gene therapy in rats with a novel nonviral gene delivery system (GE7) via intraovarian artery perfusion approach

No evidence for germ‐line transmission following prenatal and early postnatal AAV‐mediated gene delivery

Gene Expression in Macrophage-Rich Inflammatory Cell Infiltrates in Human Atherosclerotic Lesions as Studied by Laser Microdissection and DNA Array

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