Transfection of Antisense Chorionic Gonadotropin β Gene into Choriocarcinoma Cells Suppresses the Cell Proliferation and Induces Apoptosis

Hamada, Anna Lissa; Nakabayashi, Koji; Sato, Asomi; Kiyoshi, Kenji; Takamatsu, Y.; Laoag-Fernandez, Jovelle B.; Ohara, Noriyuki; Maruo, Takeshi

doi:10.1210/jc.2004-2458

Cited by 69 publications

(49 citation statements)

References 38 publications

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“…This is a clear demonstration of the hCG need for the formation of tumors, their growth, survival and invasion in the host body. Similar results were obtained when hCG synthesis was inhibited by an antisense hCG-ß subunit DNA transfection or when hCG was neutralized by its specific antibody [18] [19].…”

Section: Why Do Gtns Overproduce Hcgsupporting

confidence: 79%

How and Why Do Gestational Trophoblastic Neoplasms Overproduce Human Chorionic Gonadotropin?

Rao¹

2015

OJOG

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Section: Why Do Gtns Overproduce Hcgsupporting

confidence: 79%

How and Why Do Gestational Trophoblastic Neoplasms Overproduce Human Chorionic Gonadotropin?

Rao¹

2015

OJOG

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“…30 The growth-promoting effect of hCG␤ can be blocked by antibodies, 31 and inhibition of hCG␤ mRNA expression with antisense RNA in choriocarcinoma cells suppresses cell proliferation and induces apoptosis. 32 Furthermore, induction of an antibody response toward hCG␤ has been shown to improve survival in patients with colorectal cancer, 33 and hCG␤-targeted immunotherapies have shown promising results in animal studies on breast cancer. 34 If these results can be confirmed, methods for sensitive quantification of hCG␤ expression will become important.…”

Section: Hcg␤ In Renal Cellmentioning

confidence: 99%

“…This is caused by expression of type I hCG␤ genes, whereas increased type II gene expression occurs in most other cancers studied. Measurement of hCG␤ expression is likely to become clinically important if therapies targeting its expression [32][33][34] prove successful for treatment of various cancers. …”

Section: Hcg␤ In Renal Cellmentioning

confidence: 99%

Expression Of Human Chorionic Gonadotropin β-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma

Hotakainen

Lintula

Ljungberg

et al. 2006

The Journal of Molecular Diagnostics

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Expression of the free ␤-subunit of human chorionic gonadotropin (hCG␤) in malignant tumors is frequently associated with aggressive disease. The pretreatment serum concentration of hCG␤ is an independent prognostic variable in renal cell carcinoma (RCC). The three so-called type II genes (hCG␤ 3/9, 5, and 8) have been shown to be up-regulated in relation to type I genes (hCG␤ 6/7) in some malignant tumors. We developed a reverse transcription-polymerase chain reaction method for quantification of relative levels of the mRNAs for the two types of hCG␤ genes and studied the association between the expression in RCC tissue (n ‫؍‬ 104) and clinical outcome. hCG␤ mRNA expression was detected in 40% (42 of 104) of the tumors, and in 40 of these (93%), this consisted of hCG␤ type I mRNA only, whereas type II hCG␤ mRNA was detected in two samples. hCG␤ mRNA expression was significantly associated with a shorter diseasespecific (log-rank P ‫؍‬ 0.023; median survival 1.4 versus 7.9 years) and overall survival (log-rank P ‫؍‬ 0.011). In a Cox regression model, stage (P < 0.0001) and hCG␤ mRNA expression (P < 0.0001) were independent prognostic variables. We conclude that expression of type I hCG␤ genes indicates adverse prognosis in

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“…HCG, through its binding to the LH/CG receptor, may augment proliferation and inhibit apoptosis in choriocarcinoma JAr cells, and that the introduction of an antisense gene may be a potential approach to the inhibition of choriocarcinoma cell growth. 15 Only small proportions of hCG-H are made in the absence of trophoblast invasion (<2% of total HCG), Four model systems have been used for studying the invasive functions of cytotrophoblast cells. All four cytotrophoblast models produce exclusively hCG-H and small amounts of HCG free b-subunit and no detectable regular HCG.…”

Section: Human Chorionic Gonadotropin (Hcg)mentioning

confidence: 99%

Elevated Human Chorionic Gonadotropin HCG Serum Level in Germ Cell Testicular Tumor

Elnour¹

2018

MOJPB

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Submit Manuscript | http://medcraveonline.com cancer diagnosis, determining prognosis, surveillance following curative surgery for cancer, Tumor markers are playing an increasingly important role in cancer detection and management.In case of testicular cancer diagnosis/case finding, staging, prognosis determination, alpha-fetoprotein (AFP), human chorionic gonadotropin (HCG), and lactate dehydrogenase (LDH) are recommended, they play an important role as serum tumor markers for in the staging and monitoring of germ cell tumors. 2But what the relationship between HCG serum levels and germ cell testicular tumors? Human chorionic gonadotropin (HCG)HCG is a glycoprotein hormone composed of two dissimilar subunits, α and β, joined noncovalently. It is produced by trophoblast tissue in pregnancy and trophoblast disease and in small amounts by certain poorly differentiated cancers. The α-subunit of HCG is similar to that of the pituitary glycoprotein hormones. It is produced principally by the trophoblastic cells of the placenta but may also be produced by nontrophoblastic tissues including normal pituitary and neoplastic cells.3 It is normal for non-pregnant women and men to make very small amounts of HCG, with blood levels less than 5mIU/ mL. If HCG is high in non-pregnant women or men, it may indicate cancer. 4 Where used in malignant causes as tumor marker which is secreted by some cancers including choriocarcinoma, endometrium, and ovarian carcinoma. HCG is elevated in most type of testicular cancer seminomas and non-seminoma germ cell testicular tumors.It has been found that HCG possesses a role in the angiogenic process in vivo and in vitro by increasing capillary formation and endothelial cell migration, also, hCG-induced neovascularization. HCG may regulate vascular neoformation through vascular endothelial growth factor (VEGF). 5HCG contains five acidic asparagines-linked sugar chains. These five sugar chains are derived by sialylation from three neutral oligosaccharides: two biantennary (N-1 and N-2) and one monoantennary (N-3) complex-type oligosaccharides. The malignant transformational change of the sugar moiety of HCG can be explained by an increase of a fucosyltransferas. 6 Which indicate that N-acetylglucosaminyltransferase IV (GnT-IV) is abnormally expressed in the malignant cells. There are independent variants of HCG, each produced by different cells with separate biological functions. Multiple molecular variants of HCG include intact HCG, nicked HCG (hCGn), free β-subunit HCG (hCGβ), nicked free β-subunit HCG (hCGβn), free α-subunit hCG (hCGα), and the β-core fragment hCG (hCGβcf). In addition, HCG is differentially glycosylated in various tissues, resulting in a range of molecular forms from hypoglycosylated to hyperglycosylated. So-called hyperglycosylated HCG (hCG-h) is probably the best known of these glycosylated variants, 8 each one of them sharing the common amino acid sequence but each differing in meric structure and carbohydrate side chain structure.Hcg that produced by trophoblast cells...

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Transfection of Antisense Chorionic Gonadotropin β Gene into Choriocarcinoma Cells Suppresses the Cell Proliferation and Induces Apoptosis

Abstract: These results suggest that hCG, through its binding to the LH/CG receptor, may augment proliferation and inhibit apoptosis in choriocarcinoma JAr cells, and that the introduction of an antisense gene may be a potential approach to the inhibition of choriocarcinoma cell growth.

Cited by 69 publications

References 38 publications

How and Why Do Gestational Trophoblastic Neoplasms Overproduce Human Chorionic Gonadotropin?

How and Why Do Gestational Trophoblastic Neoplasms Overproduce Human Chorionic Gonadotropin?

Expression Of Human Chorionic Gonadotropin β-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma

Elevated Human Chorionic Gonadotropin HCG Serum Level in Germ Cell Testicular Tumor

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