Transfection and cloning of genes for membrane antigens using the FACS

Herzenberg, Leonard A.; Hsu, Charles; Alberti, Saverio; Kavathas, Paula

doi:10.1007/bf02934526

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Over the years, the instrumentation, the cell types analyzed, and the assays performed have become more diverse and complex. [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12],[13], [14], [15] What started out as a way to count cells has developed into a technology in which tens of thousands of cells are analyzed per second for expression of multiple proteins, viability, and function.…”

Section: Introductionmentioning

confidence: 99%

A Survey on Core Flow Cytometry Facilities: Instrument Maintenance, Usage, and Funding

Priya,

Brundage,

Orlando

2023

J Biomol Tech

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Flow cytometry is a powerful tool that finds applications in various fields such as immunology, molecular biology, cancer biology, virology, and infectious disease monitoring. A significant portion of the research in these disciplines is supported by flow cytometry shared resource laboratories (SRLs). There are several types of flow cytometers available for use in SRLs, including analyzers, sorters, imaging flow cytometers, and mass cytometers. Each type has different challenges when it comes to maintenance and life expectancy. An independent online survey was conducted to better understand instrument maintenance and turnover in flow cytometry SRLs. Questions regarding instrument uptime (availability), its usage, routine maintenance, and cost associated with it were addressed. The respondents also answered questions pertaining to the frequency of deep cleaning of the instrument and quality control. In addition, the survey queried about the source of funding used to purchase the instruments and possible reasons for a replacement. Presented herein are the results compiled from 146 core facilities that provide a look at the operation within a typical SRL, with the responses reflecting researchers' experiences with handling flow cytometers.

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