Transfaunation of the ruminal fluid from cows alters ruminal microbiota structure but not dominant protozoa in healthy sheep

Bessegatto, José Antonio; Alfieri, Amauri Alcindo; Lisbôa, Júlio Augusto Naylor; Weese, J. Scott; Costa, Márcio Paulino

doi:10.1016/j.smallrumres.2020.106283

Cited by 2 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a relative lack of research on RJA as a diagnostic tool for health status in the individual ruminant, as the majority of studies focus on how to maximize the feed intake and ruminal transformation of feed into energy, 22,23 or therapeutic aspects of transfaunation. [24][25][26][27] Although there are several studies describing RJA, 5,6,[9][10][11]13,17,25 a validated RJA procedure is lacking. Subjective variables, such as color, odor, and viscosity of the RJ, have been described.…”

Section: Discussionmentioning

confidence: 99%

“…However, this bias was minimized by the quantifiable nature of the results (color change of the sample with exact time recording). There is a relative lack of research on RJA as a diagnostic tool for health status in the individual ruminant, as the majority of studies focus on how to maximize the feed intake and ruminal transformation of feed into energy, 22,23 or therapeutic aspects of transfaunation 24‐27 . Although there are several studies describing RJA, 5,6,9‐11,13,17,25 a validated RJA procedure is lacking.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of sample volume and time on rumen juice analysis in cattle

Clergue

Depenbrock

Chigerwe

2023

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: Rumen juice analysis (RJA) involves analysis of a 10mL sample within minutes after sampling. However, it can be challenging to collect 10 mL of rumen juice (RJ) from some ruminants, and clinical circumstances can delay RJA.Objectives: Quantify the effect of sample volume (2, 5, 10, 50, and 100 mL), and time-to-analysis (0, 30, and 60 minutes) on RJA.Animals: Cannulated cow.Methods: Observational experimental study. Two liters of RJ were collected at 26 separate times. The samples were subdivided into 2 duplicates of each sample volume at each sampling time; and analyzed at 0, 30, and 60 minutes after collection.Rumen juice analysis included pH measurement, methylene blue reduction time (MBRT), and protozoal motility.Results: The pH of 2 and 5 mL samples was significantly (P = .01) higher than the pH of 50 and 100 mL samples at all time points. The MBRT was significantly lower (faster bacterial reduction) for 100 mL samples compared to all other samples at 0 minute and to 2, 5, and 50 mL samples at 30 min. The pH and MBRT at 60 minutes were significantly higher than at 0 minute for all volumes (P < .05 and P < .01, respectively). For large protozoa, small sample volumes (2 and 5 mL) had significantly lower protozoal motility (scores of 5 and 4.5, respectively) compared to 100 mL samples at 60 minutes (score of 4; P < .05).Conclusions and Clinical Importance: Interpretation of RJA could be affected by small sample volumes and delays to analysis. Sample volumes of ≥10 mL analyzed within 30 minutes after collection are recommended.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of sample volume and time on rumen juice analysis in cattle

Clergue

Depenbrock

Chigerwe

2023

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

First Steps into Ruminal Microbiota Robustness

Costa-Roura

Villalba

Balcells

et al. 2022

Animals

View full text Add to dashboard Cite

Despite its central role in ruminant nutrition, little is known about ruminal microbiota robustness, which is understood as the ability of the microbiota to cope with disturbances. The aim of the present review is to offer a comprehensive description of microbial robustness, as well as its potential drivers, with special focus on ruminal microbiota. First, we provide a briefing on the current knowledge about ruminal microbiota. Second, we define the concept of disturbance (any discrete event that disrupts the structure of a community and changes either the resource availability or the physical environment). Third, we discuss community resistance (the ability to remain unchanged in the face of a disturbance), resilience (the ability to return to the initial structure following a disturbance) and functional redundancy (the ability to maintain or recover initial function despite compositional changes), all of which are considered to be key properties of robust microbial communities. Then, we provide an overview of the currently available methodologies to assess community robustness, as well as its drivers (microbial diversity and network complexity) and its potential modulation through diet. Finally, we propose future lines of research on ruminal microbiota robustness.

show abstract

Transfaunation of the ruminal fluid from cows alters ruminal microbiota structure but not dominant protozoa in healthy sheep

Cited by 2 publications

References 29 publications

Effect of sample volume and time on rumen juice analysis in cattle

Effect of sample volume and time on rumen juice analysis in cattle

First Steps into Ruminal Microbiota Robustness

Contact Info

Product

Resources

About