Transepithelial transport across Caco‐2 cell monolayers of antihypertensive egg‐derived peptides. PepT1‐mediated flux of Tyr‐Pro‐Ile

Abstract

This paper examines the in vitro transepithelial transport of antihypertensive peptides derived from egg proteins using Caco-2 cell monolayers. Ovokinin (FRADHPFL) was absorbed intact through the Caco-2 cell epithelium, although it was also susceptible to the action of brush-border aminopeptidases that yielded shorter fragments prior to their transport. The tripeptide YPI was resistant to cellular peptidases and transported through the monolayer, what suggests that the reduction in systemic blood pressure caused by this peptide may be mediated by effects at tissue level. Its pathway for transepithelial absorption was examined using inhibitors of the different mechanisms for oligopeptide transport in the intestinal tract. The main route involved in the transepithelial flux of YPI is probably the peptide H(+)-coupled transporter PepT1. These results highlight the potential of antihypertensive peptides to be used in the formulation of functional foods.

Transport of ACE Inhibitory Peptides Ile‐Gln‐Pro and Val‐Glu‐Pro Derived from Spirulina platensis Across Caco‐2 Monolayers

“…(, ) to reduce cell injury resulting from high concentration. Based on our findings as well as the concentrations of VPP, YPI, and other tripeptides used in previously described transport experiments (Miguel et al., ; Satake et al., ), 1 mM was chosen as the concentration of IQP and VEP to be applied to the AP chamber in subsequent experiments.…”

Hydrophobicity exerts different effects on bioavailability and stability of antioxidant peptide fractions from casein during simulated gastrointestinal digestion and Caco-2 cell absorption

“…Interestingly, the PN content of adsorbate of HC-F1 (HC-F1-C) was lowest among the three adsorbates. This phenomenon might due to the fact that oligopeptides with high hydrophobicity have excellent affinity toward cell membranes, causing more peptides through the Caco-2 cell monolayer (Miguel et al, 2008).…”

“…Only the Caco-2 monolayer in a 6-well transwell cell culture companion plate with a TEER higher than 400 Ω was used for cell permeability test (Miguel et al, 2008).…”

Angiotensin-I-Converting Enzyme (ACE)-Inhibitory Peptides from Plants

“…Although the stability of the ACE-inhibitory peptide using Caco-2 cells has been investigated, these studies are limited. Most studies on the bioavailability of ACE-inhibitory peptides using Caco-2 cells have been focused on milk [108,113] or egg [107,114] based peptides.…”