Transepithelial permeability in the rabbit pancreas

Jansen, J.W.C.M.; Pont, J.J.H.H.M. de; Bonting, S.L.

doi:10.1016/0005-2736(79)90356-0

Cited by 37 publications

(20 citation statements)

References 26 publications

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“…In previous studies (Schreurs, Swarts, de Pont & Bonting, 1975;Jansen, de Pont & Bonting, 1979;Jansen, Fleuren-Jacobs, de Pont & Bonting, 1980) we have found that the transepithelial permeability of the isolated rabbit pancreas is high. This conclusion was reached by means of a tracer technique, in which radioactive substances are added to the bathing medium with subsequent determination of their steady-state concentration ratio in secreted fluid and bathing medium.…”

Section: Introductionmentioning

confidence: 57%

“…The pancreas of male and female New Zealand white rabbits of 2-3 kg was prepared, mounted and incubated in a balanced Krebs-Ringer bicarbonate medium (KRB medium), essentially as described by Rothman ( 1964) and modified by us (Schreurs et al 1975;Jansen et al 1979;. [L4C]sucrose (381 Ci/mol) was obtained from the Radiochemical Centre, Amersham.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have shown that the permeability of the isolated rabbit pancreas for small non-electrolytes is markedly increased after addition of carbachol or pancreozymin (Jansen et al 1979). The largest effect was obtained for sucrose, where the permeability increased by a factor of 5-4 after addition of 10-5 M-carbachol.…”

Section: Reflexion Coefficients After Carbachol Stimulationmentioning

confidence: 96%

“…Although sucrose is not completely impermeable in the isolated rabbit pancreas (Jansen et al 1979), its permeability is sufficiently low (3.5 %) to serve as the 'impermeable' reference substance. This choice allows, moreover, comparison of our measurements of the reflexion coefficients of non-electrolytes with those obtained by Dewhurst et at.…”

Section: Reflexion Coefficients For Non-electrolytesmentioning

confidence: 99%

“…We were able to establish that the high transepithelial permeability for Ca2+, Mg2+, mannitol, lactose and sucrose is due to their transport via the paracellular pathway, leading between adjacent ductular or acinar cells or both and through their tight junctions. The permeability of the paracellular pathway is increased by the stimulants of the S. L. BONTING AND OTHERS enzyme secretion process, carbachol and pancreozymin (Schreurs et al 1975;Schreurs, Swarts, de Pont & Bonting, 1976;Jansen et al 1979), but not as a result of their stimulating effect on the enzyme secretion as such (Jansen et al 1979;Jansen et al 1980). Dewhurst, Hadi, Hutson & Scratcherd (1978) measured the transepithelial permeability in the isolated perfused cat pancreas by means of the 'reflexion coefficient method'.…”

Section: Introductionmentioning

confidence: 99%

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The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas.

Bonting¹,

Pont²,

Fleuren-Jakobs³

et al. 1980

The Journal of Physiology

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SUMMARY1. The reflexion coefficients of a number of non-electrolytes and electrolytes have been determined in the isolated rabbit pancreas.2. The reflexion coefficients of the following non-electrolytes were: urea, -0-02; glycerol, 0-06; erythritol, 011; sorbitol, 0*41; mannitol, 0-42; arabinose, 0-72; xylose, 0-74, assuming a value of 1x00 for sucrose.3. These values are equal within the experimental error to values previously obtained with a tracer technique for the same preparation, but they are significantly lower than those reported by other investigators for the isolated perfused cat pancreas.4. Addition of 100 mM-sucrose to the bathing medium resulted in proportionally increased Na+ and K+ concentrations in the secreted fluid. The secreted fluid remained isotonic with the bathing medium under all circumstances.5. Addition of 10-5 M-carbachol to the bathing medium led to a reduction in the reflexion coefficient of sucrose from 1 to 0-85, but only when 25 mM-sucrose was used.6. The reflexion coefficients of electrolytes were: NaCl, 0 50; KCl, 0-51; NaHCO3, 0*52 and choline chloride, 1-02.7. It is concluded that the isolated rabbit pancreas is highly permeable, both to electrolytes and to small non-electrolytes, probably being more leaky than any other epithelium studied so far.

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Section: Introductionmentioning

confidence: 57%

Section: Methodsmentioning

confidence: 99%

Section: Reflexion Coefficients After Carbachol Stimulationmentioning

confidence: 96%

Section: Reflexion Coefficients For Non-electrolytesmentioning

confidence: 99%