Transepithelial migration of mucosal hemocytes in Crassostrea virginica and potential role in Perkinsus marinus pathogenesis

Lau, Yuk-Ting; Gambino, Laura; Santos, Bianca Silva; Espinosa, Emmanuelle Pales; Allam, Bassem

doi:10.1016/j.jip.2018.03.004

Cited by 19 publications

(14 citation statements)

References 43 publications

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“…We also stand with the argument expressed in a previous paper that the identification of a specific parasite in the type of host is a proxy for infection/colonization independently of whether the oyster manifests signs of the disease [7]. In brief, the parasite adherence to the host is part of the infection cycle [32][33][34][35][36] since the parasite changes the gene expression once it reaches the pallial cavity and translocates throughout the host's soft body [37,38]. We should also note that to date, all the Perkinsus spp.…”

Section: Discussionmentioning

confidence: 86%

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A qPCR-Based Survey of Haplosporidium nelsoni and Perkinsus spp. in the Eastern Oyster, Crassostrea virginica in Maine, USA

et al. 2020

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Eastern oyster (Crassostrea virginica) aquaculture is increasingly playing a significant role in the state of Maine’s (USA) coastal economy. Here, we conducted a qPCR-based survey for Haplosporidium nelsoni, Perkinsus marinus, and Perkinsus chesapeaki in C. virginica (n = 1440) from six Maine sites during the summer–fall of 2016 and 2017. In the absence of reported die-offs, our results indicated the continued presence of the three protozoan parasites in the six sites. The highest H. nelsoni qPCR-prevalence corresponded to Jack’s Point and Prentiss Island ( x ¯ = 40 and 48% respectively), both located in the Damariscotta River Estuary. Jack’s Point, Prentiss Island, New Meadows River, and Weskeag River recorded the highest qPCR-prevalence for P. marinus (32–39%). While the P. marinus qPCR-prevalence differed slightly for the years 2016 and 2017, P. chesapeaki qPCR-prevalence in 2016 was markedly lower than 2017 (<20% at all sites versus >60% at all sites for each of the years, respectively). Mean qPCR-prevalence values for P. chesapeaki over the two-year study were ≥40% for samples from Jack’s Point (49%), Prentiss Island (44%), and New Meadows River (40%). This study highlights that large and sustained surveys for parasitic diseases are fundamental for decision making toward the management of the shellfish aquaculture industry, especially for having a baseline in the case that die-offs occur.

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Section: Discussionmentioning

confidence: 86%

“…We should also note that to date, all the Perkinsus spp. have been described as parasites of mollusks and they have evolved to adhere to, enter the oyster, and survive inside the defense cells of mollusks [35,36,39] (reviewed in [40]). Consequently, the protozoan detected in the oysters can only come from infected oysters.…”

Section: Discussionmentioning

confidence: 99%

A qPCR-Based Survey of Haplosporidium nelsoni and Perkinsus spp. in the Eastern Oyster, Crassostrea virginica in Maine, USA

et al. 2020

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“…From the above, it's highly important to note the ability of "peripheral" hemocytes of the eastern oyster Crassostrea virginica (described as pallial hemocytes; compared to those designated as sessile hemocytes in crustaceans) to ensure exchanges with circulatory hemocytes (111,112). Such exchanges have been described as bi-directional transepithelial migrations, which proved to regulate the pathogenesis of its obligate parasite Perkinsus marinus (111,112). Interestingly, it has been demonstrated that such peripheral hemocytes share common general characteristics with circulating ones but also display some differences in their cell surface phenotypes (112)(113)(114).…”

Section: Sessile Hemocytes-potential Role In the Immune Responsementioning

confidence: 99%

“…Such exchanges have been described as bi-directional transepithelial migrations, which proved to regulate the pathogenesis of its obligate parasite Perkinsus marinus (111,112). Interestingly, it has been demonstrated that such peripheral hemocytes share common general characteristics with circulating ones but also display some differences in their cell surface phenotypes (112)(113)(114). Overall, reporting the characteristics and role of sessile hemocytes in crayfish defense strategies would help in improving the understanding of the crayfish immune response.…”

Section: Sessile Hemocytes-potential Role In the Immune Responsementioning

confidence: 99%

“…Recent studies have aimed at investigating the immune functions of sessile hemocytes from three different organs of Kuruma shrimp and have demonstrated the fact that such hemocytes differentially expressed immune-related genes compared to circulating hemocytes ( 108 , 109 ). From the above, it’s highly important to note the ability of “peripheral” hemocytes of the eastern oyster Crassostrea virginica (described as pallial hemocytes; compared to those designated as sessile hemocytes in crustaceans) to ensure exchanges with circulatory hemocytes ( 111 , 112 ). Such exchanges have been described as bi-directional transepithelial migrations, which proved to regulate the pathogenesis of its obligate parasite Perkinsus marinus ( 111 , 112 ).…”

Section: Integration Of the Crayfish’ Immune Components Into Functional Mechanismsmentioning

confidence: 99%

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A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

Bouallegui

2021

Front. Immunol.

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Freshwater crayfish immunity has received great attention due to the need for urgent conservation. This concern has increased the understanding of the cellular and humoral defense systems, although the regulatory mechanisms involved in these processes need updating. There are, however, aspects of the immune response that require clarification and integration. The particular issues addressed in this review include an overall description of the oomycete Aphanomyces astaci, the causative agent of the pandemic plague disease, which affects freshwater crayfish, and an overview of crustaceans’ immunity with a focus on freshwater crayfish. It includes a classification system of hemocyte sub-types, the molecular factors involved in hematopoiesis and the differential role of the hemocyte subpopulations in cell-mediated responses, including hemocyte infiltration, inflammation, encapsulation and the link with the extracellular trap cell death pathway (ETosis). In addition, other topics discussed include the identity and functions of hyaline cells, the generation of neoplasia, and the emerging topic of the role of sessile hemocytes in peripheral immunity. Finally, attention is paid to the molecular execution of the immune response, from recognition by the pattern recognition receptors (PRRs), the role of the signaling network in propagating and maintaining the immune signals, to the effector elements such as the putative function of the Down syndrome adhesion molecules (Dscam) in innate immune memory.

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Scallop Immunology

González

Muñoz

Brokordt

et al. 2019

Reference Module in Life Sciences

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Transepithelial migration of mucosal hemocytes in Crassostrea virginica and potential role in Perkinsus marinus pathogenesis

Cited by 19 publications

References 43 publications

A qPCR-Based Survey of Haplosporidium nelsoni and Perkinsus spp. in the Eastern Oyster, Crassostrea virginica in Maine, USA

A qPCR-Based Survey of Haplosporidium nelsoni and Perkinsus spp. in the Eastern Oyster, Crassostrea virginica in Maine, USA

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

Scallop Immunology

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