Transduction patterns and efficiencies in rabbit uterine tissues after intraluminal uterine adenovirus administration vary with the reproductive cycle

Laurema, Anniina; Lumme, Satu; Heinonen, Suvi; Heinonen, Seppo; Ylä‐Herttuala, Seppo

doi:10.1080/00016340701415640

Cited by 6 publications

(6 citation statements)

References 21 publications

(26 reference statements)

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“…On the contrary, in young animals or during di-oestrous the epithelium is inactive, and during prooestrous or oestrous the epithelium is in a growing process. Studies of transfection in the rabbit uterus have shown that the transduction pattern of uterine tissues varies significantly with the reproductive cycle (Laurema et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

Esponda

2018

Int J Med Surg Sci

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This paper deals with the efficiency of in vivo gene transfer to the mouse cauda epididymis and its relation to androgens. Previous experiments in the female reproductive tract have indicated that the efficiency of transfection is related to the hormonal stage of the animal, nevertheless no analysis have been done in the male tract. We used in vivo gene transfer to the mouse cauda epididymis employing a gene construction that expresses the Green Fluorescent Protein (GFP). Untreated and Testosterone treated males were employed. Testosterone injections (5 μg/g weight) were done from 2 days before the gene transfer, and treatment continued each day during a total period of 15 days. Fluorescence microscopy observations showed the expression of GFP in the cytoplasm of the principal cells in the epididymal tubules. The application of the QWin Program that measures the percentage of fluorescent areas showed that they are increased in the epididymis of treated males. This increase was particularly observed two days after gene injections (from 32.24 % in untreated animals to 47.62 % in testosterone treated males) and after seven days (from 29.98 % to 43.05 %). The possibility to improve transfection efficiency would increase the knowledge on epididymal physiology and would permit to modify the fertilizing capacity in mammals.

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Section: Discussionmentioning

confidence: 99%

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

Esponda

2018

Int J Med Surg Sci

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“…During the latter two stages, the epithelial conditions are very similar, and there are high concentrations of progesterone and lower concentrations of estradiol (Relloso & Esponda, 2000) (Table 2). Recently, studies of transfection in the rabbit uterus have shown that the transduction pattern of uterine tissues varies significantly with the reproductive cycle (Laurema et al ., 2007).…”

Section: Gene Transfer To the Female Genital Tractmentioning

confidence: 99%

Gene transfer to the mammalian reproductive tract

Esponda

2010

Zygote

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This review summarizes the results of research on gene transfer to the mammalian genital tract. Gene transfer experiments have been developed during the last 2 decades and have been applied using in vitro, ex vivo and in vivo procedures. (i) In vitro methods have been applied to the uterine epithelial cells with the principal purpose of analysing some pathological change occurring in the uterus. In the male tract, epididymal cell lines have been used to evaluate the expression of particular genes and the function of specific proteins. (ii) Ex vivo methods have been applied to both the uterus and the vas deferens in humans, and good transgene expression has been recorded. (iii) In vivo gene transfer in the female tract has been employed in the uterus and oviduct using gene injections or electroporation methods. The glandular epithelium of both organs can be transfected efficiently, and transfection efficiency depends on the hormonal stage of the animal. The best expression occurred during pseudopregnancy and meta-estrus periods, when high progesterone and low estradiol concentrations occur. In the male tract, in vivo methods have been applied to mouse vas deferens and epididymis. In both organs, patches of epithelial regions appeared to express the transgenes. Furthermore, the secretions of both organs were also modified using gene constructions that led to the expression of some secretory proteins. In summary, gene modifications in the epithelium of the mammalian reproductive tract have been successful employing different technologies. Further improvements in transfection efficiency would help provide new insights into the physiology of these reproductive organs. Furthermore, the use of these methods could also be used to modify the fertility of mammals.

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“…Uterine in vivo gene transfer has been demonstrated in mice (Charnock-Jones et al, 1997;Kimura et al, 2005;Rodde et al, 2008) and rabbits (Laurema et al, 2007). However, accurate access to the lumen of uterus in small animals requires invasive surgical procedures (Ngô-Muller & Muneoka, 2010).…”

Section: In Vivo Gene Delivery To the Uterusmentioning

confidence: 99%