Transduction of linked chromosomal genes between Pseudomonas aeruginosa strains during incubation in situ in a freshwater habitat

Saye, D J; Ogunseitan, Oladele A.; Sayler, Gary S.; Miller, Russell

doi:10.1128/aem.56.1.140-145.1990

Cited by 81 publications

(50 citation statements)

References 16 publications

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“…Transduction rates have not been often determined in natural environments and were mainly investigated as tools for studying bacterial genetics or for the risk assessment of the spread of genes from genetically engineered microorganisms. Most studies from lake water were performed by Miller and co-workers [281,284,450,451] by using microcosms and P. aeruginosa as system. However, transduction was also studied in a number of microcosms obtained from soils and the sea [452][453][454][455].…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

Ecology of prokaryotic viruses

2004

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The finding that total viral abundance is higher than total prokaryotic abundance and that a significant fraction of the prokaryotic community is infected with phages in aquatic systems has stimulated research on the ecology of prokaryotic viruses and their role in ecosystems. This review treats the ecology of prokaryotic viruses ('phages') in marine, freshwater and soil systems from a 'virus point of view'. The abundance of viruses varies strongly in different environments and is related to bacterial abundance or activity suggesting that the majority of the viruses found in the environment are typically phages. Data on phage diversity are sparse but indicate that phages are extremely diverse in natural systems. Lytic phages are predators of prokaryotes, whereas lysogenic and chronic infections represent a parasitic interaction. Some forms of lysogeny might be described best as mutualism. The little existing ecological data on phage populations indicate a large variety of environmental niches and survival strategies. The host cell is the main resource for phages and the resource quality, i.e., the metabolic state of the host cell, is a critical factor in all steps of the phage life cycle. Virus-induced mortality of prokaryotes varies strongly on a temporal and spatial scale and shows that phages can be important predators of bacterioplankton. This mortality and the release of cell lysis products into the environment can strongly influence microbial food web processes and biogeochemical cycles. Phages can also affect host diversity, e.g., by 'killing the winner' and keeping in check competitively dominant species or populations. Moreover, they mediate gene transfer between prokaryotes, but this remains largely unknown in the environment. Genomics or proteomics are providing us now with powerful tools in phage ecology, but final testing will have to be performed in the environment.

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Section: Horizontal Gene Transfermentioning

confidence: 99%

Ecology of prokaryotic viruses

2004

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“…For example, the coordinated release of cytotoxic phage-encoded exotoxins at either the preingestion or post-ingestion stage is one of the most effective antipredator defence strategies (Matz and Kjelleberg, 2005, Brüssow, 2007, Lainhart et al, 2009, Stolfa and Koudelka, 2012, Arnold and Koudelka, 2014. Because the worldwide phage population is >10 30 (Bergh et al, 1989, Saye et al, 1990, Maranger and Bird, 1996, Danovaro and Serresi, 2000, Weinbauer, 2004, Hewson et al, 2001, and~15% of all phage/prophage encode an exotoxin gene (Casas et al, 2006, Smith et al, 2009), exotoxin release is not only an effective antipredator defence strategy, but a commonly used one as well.…”

Section: Introductionmentioning

confidence: 99%

Determinants that govern the recognition and uptake ofEscherichia coliO157 : H7 byAcanthamoeba castellanii

Arnold

Spacht

Koudelka

2016

Cellular Microbiology

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Predation by phagocytic predators is a major source of bacterial mortality. The first steps in protozoan predation are recognition and consumption of their bacterial prey. However, the precise mechanisms governing prey recognition and phagocytosis by protists, and the identities of the molecular and cellular factors involved in these processes are, as yet, ill-characterized. Here, we show that that the ability of the phagocytic bacterivorous amoebae, Acanthamoeba castellanii, to recognize and internalize Escherichia coli, a bacterial prey, varies with LPS structure and composition. The presence of an O-antigen carbohydrate is not required for uptake of E. coli by A. castellanii. However, O1-antigen types, not O157 O-antigen types, inhibit recognition and uptake of bacteria by amoeba. This finding implies that O-antigen may function as an antipredator defence molecule. Recognition and uptake of E. coli by A. castellanii is mediated by the interaction of mannose-binding protein located on amoebae's surface with LPS carbohydrate. Phagocytic mammalian cells also use mannose-binding lectins to recognize and/or mediate phagocytosis of E. coli. Nonetheless, A. castellanii's mannose binding protein apparently displays no sequence similarity with any known metazoan mannose binding protein. Hence, the similarity in bacterial recognition mechanisms of amoebae and mammalian phagocytes may be a result of convergent evolution.

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“…Transduction has now been shown to be a significant mediator of gene transfer within several natural ecosystems, including soils (Zeph et al 1988;Stotzky 1989;Zeph & Stotzky 1989), plant surfaces (Kidambi ct a / . 1993), freshwater environments (Morrison et al 1978;Saye et al 1987Saye et al ,1990, and animals (Jarolmen et a / . 1965;Novick & Morse 1967;Baross e t a / .…”

Section: Introductionmentioning

confidence: 99%

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

1994

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A pseudolysogenic, generalized transducing bacteriophage, UT1, isolated from a natural freshwater habitat, is capable of mediating the transfer of both chromosomal and plasmid DNA between strains of Pseudomonas aeruginosa. Several chromosomal alleles from three different P. aeruginosa strains were found to transduce at frequencies from 10(-8) to 10(-10) transductants per PFU at multiplicities of infection (MOI) between 0.1 and 1. Transduction frequencies of certain alleles increased up to 1000-fold as MOIs were decreased to 0.01. UT1 is also capable of transducing plasmid DNA to indigenous populations of microorganisms in natural lake-water environments. Data obtained in this study suggest that environmentally endemic bacteriophages such as UT1 are formidable transducers of naturally occurring microbial communities. It should be possible to develop model systems to test transduction in freshwater environments using components derived exclusively from these environments.

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Transduction of linked chromosomal genes between Pseudomonas aeruginosa strains during incubation in situ in a freshwater habitat

Cited by 81 publications

References 16 publications

Ecology of prokaryotic viruses

Ecology of prokaryotic viruses

Determinants that govern the recognition and uptake ofEscherichia coliO157 : H7 byAcanthamoeba castellanii

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

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