Transduction of antibiotic resistance markers among Actinobacillus actinomycetemcomitans strains by temperate bacteriophages Aa ? 23

Willi, K.; Sandmeier, H.; Kulik, Eva M.; Meyer, Jürg

doi:10.1007/s000180050109

Cited by 44 publications

(23 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 36-kb MhaMu1 prophage sequence is complete, contains 54 CDS (MHA_1966 to MHA_2019), and is integrated into a pseudogene of an immunoglobulin metalloendopeptidase (MHA_2020). MhaMu2 is present on a 30-kb fragment encoding 46 CDS (MHA_0901 to MHA_0946), and it is integrated downstream of a possible nucleoside triphos- (58), is not associated with virulence but can transduce antibiotic resistance genes (71). A 22.5-kb scaffold of the M. haemolytica genome containing 49 CDS (MHA_2429 to MHA_2477) includes several orthologs of Aa23 proteins (Fig.…”

Section: Resultsmentioning

confidence: 99%

The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny

Gioia¹,

Qin

Jiang

et al. 2006

J Bacteriol

View full text Add to dashboard Cite

The draft genome sequence of Mannheimia haemolytica A1, the causative agent of bovine respiratory disease complex (BRDC), is presented. Strain ATCC BAA-410, isolated from the lung of a calf with BRDC, was the DNA source. The annotated genome includes 2,839 coding sequences, 1,966 of which were assigned a function and 436 of which are unique to M. haemolytica. Through genome annotation many features of interest were identified, including bacteriophages and genes related to virulence, natural competence, and transcriptional regulation. In addition to previously described virulence factors, M. haemolytica encodes adhesins, including the filamentous hemagglutinin FhaB and two trimeric autotransporter adhesins. Two dual-function immunoglobulin-protease/adhesins are also present, as is a third immunoglobulin protease. Genes related to iron acquisition and drug resistance were identified and are likely important for survival in the host and virulence. Analysis of the genome indicates that M. haemolytica is naturally competent, as genes for natural competence and DNA uptake signal sequences (USS) are present. Comparison of competence loci and USS in other species in the family Pasteurellaceae indicates that M. haemolytica, Actinobacillus pleuropneumoniae, and Haemophilus ducreyi form a lineage distinct from other Pasteurellaceae. This observation was supported by a phylogenetic analysis using sequences of predicted housekeeping genes.Mannheimia haemolytica serotype A1 is the principle bacterial pathogen associated with bovine respiratory disease complex, also known as bovine shipping fever (25, 31). M. haemolytica resides in the upper respiratory tracts of healthy ruminants, although in immunocompromised animals, such as those with a preexisting viral infection, M. haemolytica can descend into the lungs, leading to pneumonia. The mortality and morbidity associated with this disease cause substantial losses to the cattle industry (25, 31). Hence, much research on M. haemolytica is directed at understanding its virulence and designing vaccines to protect cattle (31).M. haemolytica is a member of the family Pasteurellaceae, which is classified among the ␥-proteobacteria and includes human and animal pathogens of the genera Mannheimia, Pasteurella, Haemophilus, Actinobacillus, and Histophilus (31). M. haemolytica (formerly Pasteurella haemolytica) is classified in the genus Mannheimia based upon 16S rRNA sequence phylogeny and DNA-DNA hybridizations (3). The family Pasteurellaceae includes several members whose genomes have been sequenced: Haemophilus influenzae, Pasteurella multocida, Mannheimia succiniciproducens, Actinobacillus pleuropneumoniae, Histophilus somni, Haemophilus ducreyi, and Actinobacillus actinomycetemcomitans.The primary virulence factor of M. haemolytica is considered the leukotoxin. It is a calcium-dependent toxin that is a member of the RTX family of toxins. Cytolytic at low concentrations and apoptotic at high concentrations, the leukotoxin provokes an inflammatory response that can lead to intense ed...

show abstract

Section: Resultsmentioning

confidence: 99%

The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny

Gioia¹,

Qin

Jiang

et al. 2006

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Studies on saliva have isolated bacteriophages able to lysogenize specific bacterial pathogens such as Enterococcus faecalis (Bachrach et al, 2003; Stevens et al, 2009) and Aggregatibacter actinomycetemcomitans (Sandmeier et al, 1995; Willi et al, 1997). More recently direct observation and metagenomic analysis of viral plaque and saliva fractions have revealed that bacteriophages are extremely common (Al-Jarbou, 2012; Pride et al, 2012).…”

Section: Horizontal Gene Transfer In the Oral Cavitymentioning

confidence: 99%

The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome

Roberts

Kreth

2014

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The oral microbiome is composed of a multitude of different species of bacteria, each capable of occupying one or more of the many different niches found within the human oral cavity. This community exhibits many types of complex interactions which enable it to colonize and rapidly respond to changes in the environment in which they live. One of these interactions is the transfer, or acquisition, of DNA within this environment, either from co-resident bacterial species or from exogenous sources. Horizontal gene transfer in the oral cavity gives some of the resident bacteria the opportunity to sample a truly enormous metagenome affording them considerable adaptive potential which may be key to survival in such a varying environment. In this review the underlying mechanisms of HGT are discussed in relation to the oral microbiome with numerous examples described where the direct acquisition of exogenous DNA has contributed to the fitness of the bacterial host within the human oral cavity.

show abstract

“…Specific phages could carry ARGs of Gram-positive (4-6) and Gram-negative (7-10) bacteria. Although these reports do not indicate the nature of the phage particles, some authors suggest that they could have been generated by means of generalized transducing phages that can mobilize chromosomal genes and plasmids (4,8,35).…”

mentioning

confidence: 99%

Antibiotic Resistance Genes in the Bacteriophage DNA Fraction of Human Fecal Samples

Quirós

Colomer-Lluch

Martínez-Castillo

et al. 2014

Antimicrob Agents Chemother

108

View full text Add to dashboard Cite

A group of antibiotic resistance genes (ARGs) (bla TEM , bla CTX-M-1 , mecA, armA, qnrA, and qnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs. bla TEM , qnrA, and, bla CTX-M-1 were the most abundant, and armA, qnrS, and mecA were less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra-and extraintestinal environments.A ntibiotic resistance may be obtained by spontaneous mutations or acquired by the incorporation of antibiotic resistance genes (ARGs) (1). ARGs spread between cells by using genetic platforms known as mobile genetic elements (MGEs). The most commonly studied MGEs are plasmids, transposons, integrons, and, more recently, bacteriophages (2).Bacteriophages or phage-related elements carry ARGs in Gram-positive (3-6) and Gram-negative (7-10) bacteria. Recently, some studies have suggested that the role of phages carrying ARGs in the environment is much more important than previously thought (2, 11-13). Abundant ARGs have been reported in the bacteriophage DNA fraction of fecally contaminated water (14-16), and metagenomic analyses indicate that there are abundant ARGs in viral DNA (17). As a result of their higher incidence in clinical settings, much effort has been devoted to the study of plasmids, integrons, and transposons. However, there is little information on phages carrying ARGs in clinical settings.This study analyzes a group of ARGs in phage DNA isolated from stool samples. The ARGs studied include two groups of beta-lactamase genes from Gram-negative bacteria (bla TEM and bla CTX-M-1 group ); mecA, responsible for resistance to methicillin in Staphylococcus spp.; armA, a gene which confers high-level resistance to aminoglycosides in Gram-negative bacteria; and qnrA and qnrS, plasmid-mediated genes that provide some degree of reduced quinolone susceptibility.The study was performed using 80 human fecal samples from 46 females and 34 males from 6 months to 102 years of age who visited the Sant Pau Hospital (Barcelona, Spain) during a 6-month period. Stool samples were processed according to conventional protocols for the isolation of enteropathogenic bacteria, rotavirus, and adenovirus and were microscopically examined for protozoa. Only samples that were negative for these pathogens were included in the study. None of the patients selected was involved in a food-borne outbreak or showed any severe gastrointestinal pathology. To our knowledge, none of the patients were receiving antibiotic treatment during the time of the study, although previous antibiotic treatments could not be excluded.Fecal samples were homogenized to a 1:5 (wt/vol) dilution in phosphate-buffered saline (PBS) by magnetic stirring for 15 min. Fifty milliliters of the homogenate was centrifuged at 3,000 ϫg, and the phage lysate was purified and concentrated as described previously (15, 16). Phage suspensions were treated wit...

show abstract

Transduction of antibiotic resistance markers among Actinobacillus actinomycetemcomitans strains by temperate bacteriophages Aa ? 23

Cited by 44 publications

References 35 publications

The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny

The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny

The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome

Antibiotic Resistance Genes in the Bacteriophage DNA Fraction of Human Fecal Samples

Contact Info

Product

Resources

About