Transdifferentiation of pancreatic progenitor cells to hepatocyte-like cells is not serum-dependent when facilitated by extracellular matrix proteins

Gratte, Francis D.; Pasic, Sara; Olynyk, John K.; Yeoh, George; Tosh, David; Coombe, Deirdre R.; Tirnitz–Parker, Janina E.E.

doi:10.1038/s41598-018-22596-z

Cited by 7 publications

(3 citation statements)

References 37 publications

(47 reference statements)

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“…In contrast, transdifferentiation of hepatopancreatic stem cells from the pancreas to the liver is another possible etiology ( 32 ). Transformation from pancreatic progenitor cells into hepatocytes has already succeeded in vitro ( 33 , 34 ). It could explain the abnormal phenomenon with multipotent/stem cell theory.…”

Section: Discussionmentioning

confidence: 99%

Challenge in Diagnosis and Treatment of Ectopic Hepatocellular Carcinoma: A Case Report and Literature Review

Liu

Pan

et al. 2022

Front. Surg.

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BackgroundFindings of ectopic hepatocellular carcinoma (EHCC) have been rarely documented. Complicated clinical features and unpredictable medical prognosis make diagnosis and treatment difficult.Case PresentationWe reported a 59-year-old male patient who came to the hospital with epigastric discomfort and regurgitation of gastric acid. An enhanced CT scan revealed a 1.8 cm × 1.4 cm mass in the tail of pancreas without any positive finding in the liver. Postoperative MRI scan was performed but did not reveal any evidence of hepatic tumor. The tumor was resected in toto. Meanwhile, a 1 cm × 1 cm mass in the body of the stomach was found that was removed simultaneously. Histopathology showed that the pancreatic tumor was ectopic hepatocellular carcinoma (EHCC), and that the gastric nodule was gastrointestinal stromal tumor (GIST). The patient had an uneventful postoperative recovery. He has been living without recurrence for over 7 years since surgery. Owing to our knowledge, this is the second-longest disease-free survival time for EHCC in the literature.ConclusionHere, we present a rare case of EHCC in the pancreas, and review the current literature on EHCC. Operation was an effective treatment for patients with curable EHCC. EHCC with metastasis still needs more practice to improve the poor prognosis.

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Section: Discussionmentioning

confidence: 99%

Challenge in Diagnosis and Treatment of Ectopic Hepatocellular Carcinoma: A Case Report and Literature Review

Liu

Pan

et al. 2022

Front. Surg.

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“…Transdifferentiation is the process of cell conversion from one type to another type ( Figure 2 ). For example, pancreatic progenitor cells transdifferentiate into hepatocyte-like cells 110. Recent data clearly show that somatic cells can undergo reprograming process by exogenously introducing key transcription factors such as Sox2, Klf4, c-Myc, Oct4, Nanog, and lin-28 homolog A (LIN28) 111112.…”

Section: Transdifferentiation In Cprcmentioning

confidence: 99%

The regulatory pathways leading to stem-like cells underlie prostate cancer progression

Lin

Hsieh

2019

Asian J Androl

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Prostate cancer (PCa) is the most common cause of malignancy in males and the third leading cause of cancer mortality in the United States. The standard care for primary PCa with local invasive disease mainly is surgery and radiation. For patients with distant metastases, androgen deprivation therapy (ADT) is a gold standard. Regardless of a favorable outcome of ADT, patients inevitably relapse to an end-stage castration-resistant prostate cancer (CRPC) leading to mortality. Therefore, revealing the mechanism and identifying cellular components driving aggressive PCa is critical for prognosis and therapeutic intervention. Cancer stem cell (CSC) phenotypes characterized as poor differentiation, cancer initiation with self-renewal capabilities, and therapeutic resistance are proposed to contribute to the onset of CRPC. In this review, we discuss the role of CSC in CRPC with the evidence of CSC phenotypes and the possible underlying mechanisms.

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“…However, B13 cells readily proliferate in vitro , and following transdifferentiation into HLCs they function at a level similar to freshly isolated rat hepatocytes [22] . Recently published work also suggests B13 culture can be adapted to serum-free conditions, removing barriers to their clinical use [23] .…”

Section: Introductionmentioning

confidence: 99%

Next generation in vitro liver model design: Combining a permeable polystyrene membrane with a transdifferentiated cell line

Luetchford

Wung

Argyle

et al. 2018

Journal of Membrane Science

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Herein we describe the manufacture and characterisation of biocompatible, porous polystyrene membranes, suitable for cell culture. Though widely used in traditional cell culture, polystyrene has not been used as a hollow fibre membrane due to its hydrophobicity and non-porous structure.Here, we use microcrystalline sodium chloride (4.7 ± 1.3 µm) to control the porosity of polystyrene membranes and oxygen plasma surface treatment to reduce hydrophobicity. Increased porogen concentration correlates to increased surface pore density, macrovoid formation, gas permeability and mean pore size, but a decrease in mechanical strength. For tissue engineering applications, membranes spun from casting solutions containing 40% (w/w) sodium chloride represent a compromise between strength and permeability, having surface pore density of 208.2 ± 29.7 pores/mm2, mean surface pore size of 2.3 ± 0.7 µm, and Young's modulus of 115.0 ± 8.2 MPa.We demonstrate the biocompatibility of the material with an exciting cell line-media combination: transdifferentiation of the AR42J-B13 pancreatic cell line to hepatocyte-like cells. Treatment of AR42J-B13 with dexamethasone/oncostatin-M over 14 days induces transdifferentiation towards a hepatic phenotype. There was a distinct loss of the pancreatic phenotype, shown through loss of expression of the pancreatic marker amylase, and gain of the hepatic phenotype, shown through induction of expression of the hepatic markers transferrin, carbamoylphosphate synthetase and glutamine synthetase.The combination of this membrane fabrication method and demonstration of biocompatibility of the transdifferentiated hepatocytes provides a novel, superior, alternative design for in vitro liver models and bioartificial liver devices.

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Transdifferentiation of pancreatic progenitor cells to hepatocyte-like cells is not serum-dependent when facilitated by extracellular matrix proteins

Cited by 7 publications

References 37 publications

Challenge in Diagnosis and Treatment of Ectopic Hepatocellular Carcinoma: A Case Report and Literature Review

Challenge in Diagnosis and Treatment of Ectopic Hepatocellular Carcinoma: A Case Report and Literature Review

The regulatory pathways leading to stem-like cells underlie prostate cancer progression

Next generation in vitro liver model design: Combining a permeable polystyrene membrane with a transdifferentiated cell line

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