Transdifferentiation of mesenchymal stem cells into Schwann cell-like myelinating cells

Keilhoff, Gerburg; Goihl, Alexander; Langnäse, Kristina; Fansa, Hisham; Wolf, Gerald

doi:10.1016/j.ejcb.2005.09.021

Cited by 204 publications

(158 citation statements)

References 61 publications

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“…SCs, MSCs, embryonic stem cells, marrow stromal cells are the most studied support cells candidates. Schwann cell transplantation enhances axon outgrowth both in vitro ( Schlosshauer et al, 2003) and in vivo ( Keilhoff et al, 2006). Transplantation of viable SCs offers better results than the sheer release of growth factors, because SCs have the above mentioned regeneration promoting effect ( Bhatheja and Field, 2006;Fansa et al, 1999;Hall, 1978;Ide, 1996;Madduri and Gander, 2010;Muir, 2010;Schmitte et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

et al. 2012

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Cellular systems implanted into an injured nerve may produce growth factors or extracellular matrix molecules, modulate the inflammatory process and eventually improve nerve regeneration. In the present study, we evaluated the therapeutic value of human umbilical cord matrix MSCs (HMSCs) on rat sciatic nerve after axonotmesis injury associated to Vivosorbs membrane. During HMSCs expansion and differentiation in neuroglial-like cells, the culture medium was collected at 48, 72 and 96 h for nuclear magnetic resonance (NMR) analysis in order to evaluate the metabolic profile. To correlate the HMSCs ability to differentiate and survival capacity in the presence of the Vivosorbs membrane, the [Ca 2þ ]i of undifferentiated HMSCs or neuroglial-differentiated HMSCs was determined by the epifluorescence technique using the Fura-2AM probe. The Vivosorbs membrane proved to be adequate and used as scaffold associated with undiffer-entiated HMSCs or neuroglial-differentiated HMSCs. In vivo testing was carried out in adult rats where a sciatic nerve axonotmesis injury was treated with undifferentiated HMSCs or neuroglial differentiated HMSCs with or without the Vivosorbs membrane. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index (SFI), extensor postural thrust (EPT), and withdrawal reflex latency (WRL).Stereological analysis was carried out on regenerated nerve fibers. In vitro investigation showed the formation of typical neuroglial cells after differentiation, which were positively stained for the typical specific neuroglial markers such as the GFAP, the GAP-43 and NeuN.NMR showed clear evidence that HMSCs expansion is glycolysis-dependent but their differentiation requires the switch of the metabolic profile to oxidative metabolism. In vivo studies showed enhanced recovery of motor and sensory function in animals treated with transplanted undifferentiated and differentiated HMSCs that was accompanied by an increase in myelin sheath. Taken together, HMSC from the umbilical cord Wharton jelly might be useful for improving the clinical outcome after peripheral nerve lesion.

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Section: Introductionmentioning

confidence: 99%

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

et al. 2012

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“…SCs are crucially involved into the set-up of the pro-regeneration milieu within the distal nerve segments and the potential of nerve autotransplantation is mainly attributed to the presence of this cell type within the grafts. 5,9 BMSCs have been described as attributable to in vitro transdifferentiation into SC-like cells, 10,11 a feature that would allow to include SC properties with the benefit of avoiding autologous SC harvest. Additionally, despite their putative potential for transdifferentiation, mesenchymal stromal cells could serve as living mini-pumps for regeneration-promoting factors.…”

mentioning

confidence: 99%

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Wróbel

Serra

Ribeiro-Samy

et al. 2014

Tissue Engineering Part A

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Natural biomaterials have attracted an increasing interest in the field of tissue-engineered nerve grafts, representing a possible alternative to autologous nerve transplantation. With the prospect of developing a novel entubulation strategy for transected nerves with cell-seeded chitosan films, we examined the biocompatibility of such films in vitro. Different types of rat Schwann cells (SCs)-immortalized, neonatal, and adult-as well as rat bone-marrow-derived mesenchymal stromal cells (BMSCs) were analyzed with regard to their cell metabolic activity, proliferation profiles, and cell morphology after different time points of mono-and cocultures on the chitosan films. Overall the results demonstrate a good cytocompatibility of the chitosan substrate. Both cell types were viable on the biomaterial and showed different metabolic activities and proliferation behavior, indicating cell-type-specific cell-biomaterial interaction. Moreover, the cell types also displayed their typical morphology. In cocultures adult SCs used the BMSCs as a feeder layer and no negative interactions between both cell types were detected. Further, the chitosan films allow neurite outgrowth from dissociated sensory neurons, which is additionally supported on film preseeded with SC-BMSC cocultures. The presented chitosan films therefore demonstrate high potential for their use in tissue-engineered nerve grafts.

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“…They are able to differentiate into bone, tendon, cartilage and fat (Pittenger et al 1999) or under specific condition into neuronal, muscle, liver cells (Keilhoff et al 2006;Yu et al 2007;Greco & Rameshwar, 2008) as well as epithelial cells of lung, skin, kidney and gastrointestinal tract (Herzog et al 2003). The first evidence for the existence of non-hematopoietic stem cells derived from bone marrow has been available from Friedenstein's work in 1970s (Friedenstein et al 1976).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

“…For example, spinal ischemia-induced spastic paraplegia which is associated with a selective loss of small inhibitory interneurons, would necessarily involve implantation of neuronal progenitors. On the other hand, diseases or spinal cord trauma, with different pathological outcome, resulting in demyelination of axons followed by destruction of long descending tracts would rather require transplantation of myelin-producing cells such as oligodendroglial cells, Schwann cells or Olfactory ensheating cells Keilhoff et al 2006;Pearse et al 2007;Raisman, 2007). Since a well-documented repertoire of specific surface markers for cells of NSCs at different developmental stages have been identified, it may be possible to identify factors which affect their commitment to oligodendroglial cells or neurons and combine this with optimal sorting methods (Deng & Poretz, 2003;Pruszak et al 2007;Uchida et al 2000).…”

Section: Transplantation Strategies Utilizing Nscsmentioning

confidence: 99%

Mesenchymal Stromal Cells and Neural Stem Cells Potential for Neural Repair in Spinal Cord Injury and Human Neurodegenerative Disorders

Čı́žková¹,

Žilka²,

Kazmerova³

et al. 2012

Neural Stem Cells and Therapy

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Transdifferentiation of mesenchymal stem cells into Schwann cell-like myelinating cells

Cited by 204 publications

References 61 publications

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Mesenchymal Stromal Cells and Neural Stem Cells Potential for Neural Repair in Spinal Cord Injury and Human Neurodegenerative Disorders

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