Transdetermination: A New Trend in Cellular Reprogramming

Manohar, Rohan; Lagasse, Eric

doi:10.1038/mt.2009.93

Cited by 15 publications

(5 citation statements)

References 20 publications

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“…Some define it as a process without an intermediate cell type, namely a direct conversion of one differentiated cell type to another (e.g. [ 16 ]). Others are agnostic and call it transdifferentiation even if there is an intermediate state involved (e.g.…”

Section: What Is Transdifferentation?mentioning

confidence: 99%

Cellular plasticity, caspases and autophagy; that which does not kill us, well, makes us different

2018

Open Biol.

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The ability to regenerate is a fundamental requirement for tissue homeostasis. Regeneration draws on three sources of cells. First and best-studied are dedicated stem/progenitor cells. Second, existing cells may proliferate to compensate for the lost cells of the same type. Third, a different cell type may change fate to compensate for the lost cells. This review focuses on regeneration of the third type and will discuss the contributions by post-transcriptional mechanisms including the emerging evidence for cell-autonomous and non-lethal roles of cell death pathways.

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Section: What Is Transdifferentation?mentioning

confidence: 99%

Cellular plasticity, caspases and autophagy; that which does not kill us, well, makes us different

2018

Open Biol.

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“…New approaches may be developed in the near future that may be even more effective than our present technology of differentiating hepatocytes. For example, recent reports have shown the transdifferentiation of fibroblasts to neuronal cells and cardiomyocytes [ 108 – 111 ]. Thus one of the future approaches may be the similar direct differentiation of fibroblasts to hepatocytes or hepatic progenitor cells.…”

Section: Future Challengesmentioning

confidence: 99%

New Approaches in the Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Hepatocytes

Behbahan

Duan

Lam

et al. 2011

Stem Cell Rev and Rep

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Orthotropic liver transplantation is the only established treatment for end-stage liver diseases. Utilization of hepatocyte transplantation and bio-artificial liver devices as alternative therapeutic approaches requires an unlimited source of hepatocytes. Stem cells, especially embryonic stem cells, possessing the ability to produce functional hepatocytes for clinical applications and drug development, may provide the answer to this problem. New discoveries in the mechanisms of liver development and the emergence of induced pluripotent stem cells in 2006 have provided novel insights into hepatocyte differentiation and the use of stem cells for therapeutic applications. This review is aimed towards providing scientists and physicians with the latest advancements in this rapidly progressing field.

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“…Interestingly, somatic cells also can be converted into different specialized cells using direct lineage conversion or direct reprogramming and/or transdifferentiation in the absence of a pluripotent state. These methods utilize forced expression of lineage-instructive transcription factors as a strategy for the generation of various cell types [61,[169][170][171][172][173] by bypassing the multiple steps of lineage specification during development [174]. Direct reprogramming using lineage-instructive transcription factors was first done by the conversion of fibroblasts into myoblasts by overexpressing Myod [4], and subsequent studies showed the feasibility of this strategy [175][176][177][178].…”

Section: Cell Fate Conversionmentioning

confidence: 99%

Engineering Cell Fate: The Roles of iPSC Transcription Factors, Chemicals, Barriers and Enhancing Factors in Reprogramming and Transdifferentiation

Ebrahimi

2015

Preprint

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Direct reprogramming technology has emerged as an outstanding technique for the generation of induced pluripotent stem (iPS) cells and various specialized cells directly from somatic cells of different species. Recent studies dissecting the molecular mechanisms of reprogramming have methodologically improved the quality, ease and efficiency of reprogramming and eliminated the need for genome modifications with integrating viral vectors. With these advancements, direct reprogramming technology has moved closer to clinical application. Here, we provide a comprehensive overview of the cutting-edge findings regarding distinct barriers of reprogramming to pluripotency, strategies to enhance reprogramming efficiency, and chemical reprogramming as one of the non-integrating approaches in iPS cell generation. In addition to direct transdifferentiation, pluripotency factor-induced transdifferentiation or cell activation and signaling directed (CASD) lineage conversion is described as a robust strategy for the generation of both tissue-specific progenitors and clinically relevant cell types. Then, we consider the possibility that a combined method of inhibition of roadblocks (e.g. p53, p21, p57, Mbd3, etc.), and application of enhancing factors in a chemical reprogramming paradigm would be an almost safe, reliable and effective approach in pluripotent reprogramming and transdifferentiation. Furthermore, with respect to the state of native, aberrant, and target gene regulatory networks in reprogrammed cell populations, CellNet is reviewed as a computational platform capable of evaluating the fidelity of reprogramming methods and refining current engineering strategies. Ultimately, we conclude that a faithful, highly efficient and integration-free reprogramming paradigm would provide powerful tools for research studies, drug-based induced regeneration, cell transplantation therapies and other regenerative medicine purposes.

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Transdetermination: A New Trend in Cellular Reprogramming

Cited by 15 publications

References 20 publications

Cellular plasticity, caspases and autophagy; that which does not kill us, well, makes us different

Cellular plasticity, caspases and autophagy; that which does not kill us, well, makes us different

New Approaches in the Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Hepatocytes

Engineering Cell Fate: The Roles of iPSC Transcription Factors, Chemicals, Barriers and Enhancing Factors in Reprogramming and Transdifferentiation

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